Flow Injection Analysis System for Screening Organophosphorus Pesticides by their Inhibitory Effect on the Enzyme Acethylcholinesterase

Silva, Marcos P.; Tubino, Matthieu; Elsholz, Tereza C. R.; Elsholz, Olaf; Khan, Sabir; Vila, Marta Maria Duarte Carvalho

doi:10.5935/0103-5053.20150301

Cited by 4 publications

(6 citation statements)

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“…As summarized in Table 4, biosensing strategies, based on chromatographic (Bidmanova et al, 2016;Brack et al, 2002;Shimizu et al, 2013), electrochemical (Anu Prathap et al, 2012Besombes et al, 1995;Kaur et al, 2016;Kim et al, 2008;McArdle and Persaud, 1993;Miao et al, 2016;Nomngongo et al, 2012;Vidal et al, 2008;Wu, 2011;Xia et al, 2015;Xu et al, 2019), potentiometric (Hutter et al, 1995), conductimetric (Anh et al, 2004), spectrophotometric (de Souza et al, 2018Hipólito-Moreno et al, 1998;Q. Liu et al, 2019;Mazurenko et al, 2018;McArdle and Persaud, 1993;Shahar et al, 2019a;Silva et al, 2015) and fluorescence spectroscopic methods (Bidmanova et al, 2016(Bidmanova et al, , 2010Campbell et al, 2006;Mazurenko et al, 2018;Sok and Fragoso, 2018) can be found in literature. Wu (2011), for example, proposed a biosensor for determination of chlorinated pesticides, based on direct electrochemistry of house fly cytochrome 6A1 (CYP6A1), an isoenzyme from the CYP450 family.…”

Section: Enzymatic Biosensors For Hocs Determinationmentioning

confidence: 99%

“…Several examples of AChE inhibition-based biosensors can be found in literature for chlorpyrifos determination in food products including cabbage, lettuce or orange juice (Kim et al, 2018;Miao et al, 2016;Silva et al, 2015;Xia et al, 2015). The applicability of AChE-based biosensors for determination of chlorinated organophosphorus pesticides seems to be limited to single use devices, since these toxicants inhibit enzyme activity irreversibly.…”

Section: Enzymatic Biosensors For Hocs Determinationmentioning

confidence: 99%

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Enzymatic assays for the assessment of toxic effects of halogenated organic contaminants in water and food. A review

Artabe

Cunha-Silva

Barranco

2020

Food and Chemical Toxicology

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Halogenated organic compounds are a particular group of contaminants consisting of a large number of substances, and of great concern due to their persistence in the environment, potential for bioaccumulation and toxicity. Some of these compounds have been classified as persistent organic pollutants (POPs) under The Stockholm Convention and many toxicity assessments have been conducted on them previously. In this work we provide an overview of enzymatic assays used in these studies to establish toxic effects and dose-response relationships. Studies in vivo and in vitro have been considered with a particular emphasis on the impact of halogenated compounds on the activity of relevant enzymes to the humans and the environment. Most information available in the literature focuses on chlorinated compounds, but brominated and fluorinated molecules are also the target of increasing numbers of studies. The enzymes identified can be classified as enzymes: i) the activities of which are affected by the presence of halogenated organic compounds, and ii) those involved in their metabolisation/detoxification resulting in increased activities. In both cases the halogen substituent seems to have an important role in the effects observed. Finally, the use of these enzymes in biosensing tools for monitoring of halogenated compounds is described.

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Section: Enzymatic Biosensors For Hocs Determinationmentioning

confidence: 99%

Section: Enzymatic Biosensors For Hocs Determinationmentioning

confidence: 99%

Enzymatic assays for the assessment of toxic effects of halogenated organic contaminants in water and food. A review

Artabe

Cunha-Silva

Barranco

2020

Food and Chemical Toxicology

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“…1 Although the use of pesticides has significantly improved the quality as well as quantity of food to cater to the needs of the ever-growing world population, their excessive use has had undesirable effects on nontargeted organisms, including human beings. 2 It is estimated that less than 0.1% of the total pesticides applied to crops reach the targeted pests, while the rest permeate into the environment, contaminating the soil, 3 water, 4 and air. 5 Fipronil, 5-amino-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-4-[(trifluoromethyl)sulfinyl]-1H-pyrazole-3-carbonitrile, phenyl pyrazole, is considered the third most used pesticide in the world market due to its diverse scope and wide range of reactivity.…”

Section: Introductionmentioning

confidence: 99%

“…There are more than 500 different pesticides that are commonly used in our surroundings . Although the use of pesticides has significantly improved the quality as well as quantity of food to cater to the needs of the ever-growing world population, their excessive use has had undesirable effects on nontargeted organisms, including human beings . It is estimated that less than 0.1% of the total pesticides applied to crops reach the targeted pests, while the rest permeate into the environment, contaminating the soil, water, and air …”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Polymeric Sorbent for Targeted Dispersive Solid-Phase Microextraction of Fipronil from Milk Samples

Hayat

Manzoor

Raza³

et al. 2022

ACS Omega

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Fipronil, a phenyl pyrazole insecticide, is extensively used in agriculture to control insect infestation. It has the potential to assimilate into the food chain, leading to serious health concerns. We report molecularly imprinted polymer (MIP)-based dispersive solidphase microextraction for the targeted determination of fipronil in milk samples. Designing such a sorbent is of paramount importance for measuring the accurate amount of fipronil for monitoring its permissible limit. Response surface methodology based on a central composite design following a face-centered approach was used to optimize experimental conditions. The maximum binding capacity of 47 mg g −1 was achieved at optimal parameters of time (18 min), temperature (42 °C), pH (7), and analyte concentration (120 mg L −1 ). Under these conditions, a high percentage recovery of 94.6 ± 1.90% (n = 9) and a low limit of detection (LOD) and limit of quantitation (LOQ) (5.64 × 10 −6 and 1.71 × 10 −5 μg mL −1 , respectively) were obtained. The MIP was well characterized through a scanning electron microscope (SEM) as well as Brunauer−Emmett−Teller (BET), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA) methods. Adsorption kinetics of the MIP followed the pseudo-first-order model (R 2 0.99 and χ 2 0.96), suggesting the MIP−analyte interaction to be a physiosorptive process, while adsorption isotherms followed the Freundlich model (R 2 0.99). The real sample analysis through high-performance liquid chromatography (HPLC) confirmed the selective determination of fipronil from milk samples.

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“…The exponential population growth and the resulting increased demand for food in the world have led to a model of agricultural production based on the intensive use of insecticides for improving crop yields (Andreu & Picó, 2004; Kiljanek et al, 2016; Silva et al, 2015). The use of those agrochemicals is often a major concern because of the potential harm to the environment and suspected toxic effects to the health of bees, which are very sensitive to insecticides (Kiljanek et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Modified QuEChERS Method for Extracting Thiamethoxam and Imidacloprid from Stingless Bees: Development, Application, and Green Metrics

Rahman

Ali

Xavier

et al. 2022

Enviro Toxic and Chemistry

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In the present study, a method for the determination of residues of the neonicotinoid insecticides thiamethoxam and imidacloprid in the stingless bee Melipona scutellaris Latreille (1811) was optimized through a factorial design, tested using green metrics, and then applied to exposed bees. It combines the extraction with a modified quick, easy, cheap, effective, rugged, and safe method and the determination by liquid chromatography–tandem mass spectrometry analysis. Different parameters such as the mass of the sample, dispersive sorbents, and elution solvents were assessed. Method validation parameters were checked and include sensitivity, specificity, and linearity. The limit of quantification of 0.0025 μg g–1 was obtained for both insecticides, where accuracy was 94%–100% with satisfactory intraday and interday precisions (relative standard deviation <10%). The qualified method was applied to orally and topically exposed bee samples, and the results indicated that it is suitable for the determination and quantification of neonicotinoid pesticide residues in this species. Moreover, green analytical metrics like the National Environmental Methods Index, Eco Scale score, high‐performance liquid chromatography with an environmental assessment tool (HPLC‐EAT), waste generation, and amount of sample were compared with methods described in the literature involving neonicotinoid analysis in honeybees. As a result, the present study displayed the highest Eco Scale score and HPLC‐EAT score and the second smallest amount of sample and waste generated. Thus, the method meets green analytical metrics more than other methods. In this sense, besides the application, the multicriteria decision analysis tool employed suggests that this is a good option as a green analytical method. Environ Toxicol Chem 2022;41:2365–2374. © 2022 SETAC

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Flow Injection Analysis System for Screening Organophosphorus Pesticides by their Inhibitory Effect on the Enzyme Acethylcholinesterase

Cited by 4 publications

References 1 publication

Enzymatic assays for the assessment of toxic effects of halogenated organic contaminants in water and food. A review

Enzymatic assays for the assessment of toxic effects of halogenated organic contaminants in water and food. A review

Molecularly Imprinted Polymeric Sorbent for Targeted Dispersive Solid-Phase Microextraction of Fipronil from Milk Samples

Modified QuEChERS Method for Extracting Thiamethoxam and Imidacloprid from Stingless Bees: Development, Application, and Green Metrics

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