Flow Infusion ESI High Resolution Mass Spectrometry Metabolomics Analytical Platforms

Wood, Paul L.

doi:10.1007/978-1-0716-0864-7_1

Cited by 2 publications

(1 citation statement)

References 3 publications

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“…With regard to brain function, free N-acetylmethionine, Nacetylglutamine, N-acetylglutamate, N-acetylasparagine, and Nacetylalanine have all been monitored in the human brain [23,[36][37][38][39][40]. N-acetylglutamate is a critical modulator of the urea cycle, while Nacetylleucine modulates the neuronal activity of vestibulocerebellar and posterolateral thalamic circuits involved in vestibular function, while Nacetylglutamine appears to be involved in the sleep-wake cycle [40][41][42].…”

Section: Discussionmentioning

confidence: 99%

Metabolomics of Rat Brain After Treatment with Phenelzine: High-Resolution Mass Spectrometric Demonstration of Increased Brain Levels of N-Acetyl Amino Acids

Wood¹,

Cebak²,

Baker³

2020

NNB

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Background: Phenelzine (PLZ) is a non-specific monoamine oxidase inhibitor that has demonstrated clinical efficacy in patients with treatment resistant depression. The mechanism of action with regard to this efficacy is complicated in that its metabolite, β-phenylethylidenehydrazine (PEH), is an inhibitor of amino acid transaminases resulting in dramatic brain elevations of GABA, alanine, ornithine and tyrosine. The full neurochemical profile of PLZ and PEH remain to be explored. Objective: To undertake a non-targeted metabolomics study of phenelzine on rat brain neurochemistry. Methods: We undertook a high-resolution mass spectrometric metabolomics analysis of rat cortical brain 1 and 12 hours after intraperitoneal dosing with PLZ or PEH. Tandem mass spectrometry was utilized to obtain relative quantitation data. Results: N-acetyl amino acids were found to be elevated in cortical brain tissue following either PLZ or PEH treatments. Conclusions: Our data indicate PLZ treatment significantly augments brain levels of N-acetyl amino acids and that this may involve inhibition of deacylases by PEH and/or induction of N-amino acid acetyltransferases.

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Section: Discussionmentioning

confidence: 99%

Metabolomics of Rat Brain After Treatment with Phenelzine: High-Resolution Mass Spectrometric Demonstration of Increased Brain Levels of N-Acetyl Amino Acids

Wood¹,

Cebak²,

Baker³

2020

NNB

Self Cite

View full text Add to dashboard Cite

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Metabolic and Lipid Biomarkers for Pathogenic Algae, Fungi, Cyanobacteria, Mycobacteria, Gram-Positive Bacteria, and Gram-Negative Bacteria

Wood

2024

Metabolites

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The utilization of metabolomics and lipidomics analytical platforms in the study of pathogenic microbes is slowly expanding. These research approaches will significantly contribute to the establishment of microbial metabolite and lipid databases of significant value to all researchers in microbiology. In this review, we present a high-level overview of some examples of biomarkers that can be used to detect the presence of microbes, monitor the expansion/decline of a microbe population, and add to our understanding of microbe biofilms and pathogenicity. In addition, increased knowledge of the metabolic functions of pathogenic microbes can contribute to our understanding of microbes that are utilized in diverse industrial applications. Our review focuses on lipids, secondary metabolites, and non-ribosomal peptides that can be monitored using electrospray ionization high-resolution mass spectrometry (ESI-HRMS).

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Flow Infusion ESI High Resolution Mass Spectrometry Metabolomics Analytical Platforms

Cited by 2 publications

References 3 publications

Metabolomics of Rat Brain After Treatment with Phenelzine: High-Resolution Mass Spectrometric Demonstration of Increased Brain Levels of N-Acetyl Amino Acids

Metabolomics of Rat Brain After Treatment with Phenelzine: High-Resolution Mass Spectrometric Demonstration of Increased Brain Levels of N-Acetyl Amino Acids

Metabolic and Lipid Biomarkers for Pathogenic Algae, Fungi, Cyanobacteria, Mycobacteria, Gram-Positive Bacteria, and Gram-Negative Bacteria

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