Flow electrochemical biosensors based on enzymatic porous reactor and tubular detector of silver solid amalgam

Electrochemical Biosensors Based on Enzymatic Reactors Filled by Various Types of Silica and Amalgam Powders for Measurements in Flow Systems

“…The reactor with the immobilized enzyme was stored in microtube with mobile phase at 4 °C when it was not used. The results of this monitoring indicated that 77 % of the current response of glucose was retained after 35 days .…”

“…A comparison of electrochemical analysis of glucose in honey with optical methods was described in our previous paper . Determination of glucose in natural sample of honey was performed by help of the biosensor with the SBA15‐GOx reactor.…”

“…Apart of glucose the most often represented carbohydrates in honey are fructose, maltose and sucrose . Experiment with three repeated injections of 1.0×10 −3 mol L−1 solutions of these compounds were carried out in our previous publication . No changes of the baseline were observed which confirms the high selectivity of GOx.…”

“…Two main constructions of the electrochemical biosensors were used: i) biorecognition element (enzyme, avidin, oligonucleotide, etc.) is anchored at a working electrode surface either directly, or through an additional thin layer, for example of chitosan hydrogel ; ii) biorecognition element is adsorbed or covalently bound to a surface that is spatially separated from the working electrode . In the last case we talk about the use of some type of a reactor, and hence the enzymatic biosensor consists of two separate parts: the enzymatic reaction takes place in the reactor, and the detector (working electrode) then registers either some product of enzymatic reaction or, e.g., decrease/increase of oxygen concentration in the solution.…”

Behavior of metal ions in bioelectrochemical systems: A review

“…A biosensor for determination of glucose consisted of enzymatic porous reactor based on silver solid amalgam (AgSA) was constructed by Josyp cuk et al, of which high hydrogen overvoltage of the AgSA detector enabled to apply detection potential of more negative than À1.0 V. It increased the sensitivity of oxygen measurement and the covalent bonds amalgam-thiol-enzyme glucose oxidase (GOx) effectively and contributed to long-term stability [192]. Yang et al developed a bienzyme amperometric glucose biosensor with both GOx and horseradish peroxidase enzymes coimmobilized in chitosan film coated on silver nanocubes (AgNCs) modified Au electrode.…”