Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII 2020
DOI: 10.1117/12.2544759
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Flow cytometry visualization and real-time processing with a CMOS SPAD array and high-speed hardware implementation algorithm

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Cited by 3 publications
(3 citation statements)
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“…The data was read out and analysed after the measurement had finished. We note that it is in principle possible to obtain real-time FLIM image displays from average photon arrival times [ 32 , 50 57 ], but this method was not employed here.…”
Section: Resultsmentioning
confidence: 99%
“…The data was read out and analysed after the measurement had finished. We note that it is in principle possible to obtain real-time FLIM image displays from average photon arrival times [ 32 , 50 57 ], but this method was not employed here.…”
Section: Resultsmentioning
confidence: 99%
“…com/inatamara/NyxSense) and could be used by the community to test, further improve, or simply use the methodology we proposed. Spectrally resolved FLIM is readily available commercially, and several bespoke implementations aimed to make available cost-effective and user-friendly solutions have been also published (e.g., [6,42,43,51,56,58,61,63,76], promising increased availability of such sophisticated assays in the near future. We showed that the combination of spectrally resolved time phasors (sTP → ) with the spectral phasors (SP or TSP) permitted efficient demixing of three FRET pairs, presenting a low level of direct acceptor excitation using only six control signatures.…”
Section: Discussionmentioning
confidence: 99%
“…A new nonparametric empirical Bayesian framework [ 16 ] was adopted for lifetime analysis based on a statistical model, where the expectation–maximization algorithm was employed to solve the optimization problem. A hardware-friendly fitting-free center of mass (CMM) [ 17 , 18 , 19 ] algorithm was proposed to deliver fast analysis and has been applied to a flow-cytometry system [ 20 , 21 ]. Integral equation methods (IEM) [ 22 ] were also implemented in FPGA devices to provide real-time analysis.…”
Section: Introductionmentioning
confidence: 99%