Flow cytometry protocol for GLUT4-myc detection on cell surfaces
Emilia Zanni-Ruiz,
Luis Segundo Mayorga,
Martin Alejandro Pavarotti
Abstract:Insulin and muscle contraction trigger GLUT4 translocation to the plasma membrane, which increases glucose uptake by muscle cells. Insulin resistance and type 2 diabetes are the result of impaired GLUT4 translocation. Quantifying GLUT4 translocation is essential for comprehending the intricacies of both physiological and pathophysiological processes involved in glucose metabolism. The most commonly used methods for measuring GLUT4 translocation are the ELISA-type assay and the immunofluorescence assay. While s… Show more
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