Flow cytometry profiling of cellular immune response in COVID-19 infected, recovered and vaccinated individuals

Kumari, Diksha; Singh, Sayar; Kumari, Manisha; Gupta, Harshita; Chauhan, Deepika; Singh, Karuna; Eslavath, Malleswara Rao; Bhushan, Brij; Dogra, Vikas; Bargotya, Mona; Bhattar, Sonali; Gupta, Utkarsh; Hussain, Javid; Jain, S. K.; Meena, R. C.; Varshney, Rajeev K.; Ganju, Lilly; Singh, Yamini

doi:10.1016/j.imbio.2023.152392

Cited by 3 publications

(3 citation statements)

References 42 publications

(45 reference statements)

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“…A study that profiled the cellular immune responses in COVID-19 infected, recovered, and vaccinated individuals showed a significantly lower ratio of activated T-cells to total lymphocytes among patients who died from COVID-19 compared to those who were hospitalized and recovered. 107 Moreover, vaccinated individuals who experienced breakthrough infection and recovered with mild to moderate symptoms demonstrated enhanced T-cell responses when compared to those who required hospitalization. Possessing a large repertoire of T-cells is understandably a key determinant of the response to a viral infection.…”

Section: Durable Protection Against Severe Covid-19 Diseasementioning

confidence: 98%

COVID-19 vaccines: Immune correlates and clinical outcomes

Mahrokhian,

Tostanoski,

Vidal

et al. 2024

Human Vaccines & Immunotherapeutics

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Severe disease due to COVID-19 has declined dramatically as a result of widespread vaccination and natural immunity in the population. With the emergence of SARS-CoV-2 variants that largely escape vaccine-elicited neutralizing antibody responses, the efficacy of the original vaccines has waned and has required vaccine updating and boosting. Nevertheless, hospitalizations and deaths due to COVID-19 have remained low. In this review, we summarize current knowledge of immune responses that contribute to population immunity and the mechanisms how vaccines attenuate COVID-19 disease severity.

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Section: Durable Protection Against Severe Covid-19 Diseasementioning

confidence: 98%

COVID-19 vaccines: Immune correlates and clinical outcomes

Mahrokhian,

Tostanoski,

Vidal

et al. 2024

Human Vaccines & Immunotherapeutics

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“…The collective work to characterize both the efficacy and mechanism of this vaccine underpinned several clinical trials, eventually supporting the approval of Skycovione in the Republic of Korea (e.g., NCT04742738, NCT04750343, and NCT05007951). The ability to bridge assays and mechanisms between preclinical species and clinical settings further enables flow cytometric approaches as a key tool for understanding the cellular response to vaccines [64,65], which is critical to gaining a systems-level understanding of this therapeutic class [66].…”

Section: Translational Research Informing the Path To The Clinicmentioning

confidence: 99%

Applications of Flow Cytometry in Drug Discovery and Translational Research

Ullas,

Sinclair

2024

IJMS

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Flow cytometry is a mainstay technique in cell biology research, where it is used for phenotypic analysis of mixed cell populations. Quantitative approaches have unlocked a deeper value of flow cytometry in drug discovery research. As the number of drug modalities and druggable mechanisms increases, there is an increasing drive to identify meaningful biomarkers, evaluate the relationship between pharmacokinetics and pharmacodynamics (PK/PD), and translate these insights into the evaluation of patients enrolled in early clinical trials. In this review, we discuss emerging roles for flow cytometry in the translational setting that supports the transition and evaluation of novel compounds in the clinic.

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“…Numerous studies that analyzed the immune response against SARS-CoV-2 in convalescents indicate the possibility of obtaining immune protection after natural infection [10][11][12]. However, little is known about the comprehensive impact of infection on the immune system after recovery.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Leukocyte Subpopulations by Flow Cytometry during Hospitalization Depending on the Severity of COVID-19 Course

Rutkowska,

Kwiecień,

Pietruszka-Wałęka

et al. 2023

Biomedicines

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The mechanisms underlying the immune response to coronavirus disease 2019 (COVID-19) and the recovery process have not been fully elucidated. The aim of the study was to analyze leukocyte subpopulations in patients at significant time points (at diagnosis, and 3 and 6 months after infection) selected according to the analysis of changes in the lungs by the CT classification system, considering the severity of the disease. The study groups consisted of severe and non-severe COVID-19 patients. There was a significant decrease in CD8+ T cells, NK and eosinophils, with an increasing percentage of neutrophils during hospitalization. We noticed lower levels of CD4 and CD8 T lymphocytes, eosinophils, basophils, and CD16+ monocytes and elevated neutrophil levels in severe patients relative to non-severe patients. Three months after infection, we observed higher levels of basophils, and after 6 months, higher CD4/CD8 ratios and T cell levels in the severe compared to non-severe group. Non-severe patients showed significant changes in the leukocyte populations studied at time of hospitalization and both within 3 months and 6 months of onset. The CT CSS classification with parameters of the flow cytometry method used for COVID-19 patients determined changes that proved useful in the initial evaluation of patients.

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Flow cytometry profiling of cellular immune response in COVID-19 infected, recovered and vaccinated individuals

Cited by 3 publications

References 42 publications

COVID-19 vaccines: Immune correlates and clinical outcomes

COVID-19 vaccines: Immune correlates and clinical outcomes

Applications of Flow Cytometry in Drug Discovery and Translational Research

Analysis of Leukocyte Subpopulations by Flow Cytometry during Hospitalization Depending on the Severity of COVID-19 Course

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