Flow cytometry in the exploration of the physiopathology of occupational lung disease.

Abstract

Flow cytometry is a simple analytical technique used for the investigation of cells taken from various sources. Cells are identified by both their physical characteristics and the presence or absence of specific molecules on the cell surface. These molecules may be either phenotypic, or induced by a specific stimulus. Flow cytometry has been used to identify the nature and extent of the immune response in several occupational respiratory conditions including occupational asthma, irritant induced respiratory problems, and asbestos related lung disease. Also, it may be of value in monitoring workplace exposure to some hazardous materials. Although of limited diagnostic value at present, the technique has provided an insight into the modulation of immune cells, and their function, in people exposed to hazardous materials in the workplace. In this review, the principals of flow cytometry will be explored and the use of flow cytometry to investigate occupational respiratory disease will be discussed. (Occup Environ Med 1999;56:742-746)

Flow cytometry enables identification of sporophytic eliciting stress treatments in gametic cells

“…Flow cytometry has been widely applied for the analysis of animal cells (Curran, 1999) and more recently, plants cells (Doležel et al, 2007a,b) following the advent of simple and reproducible methods for tissue analysis (Galbraith et al, 1983;Doležel et al, 2007a,b). Applications to plant cell analysis include, but are not limited to, differentiation between cells within a mixed cell population, analysis of chemical composition of different tissues, measurement of secondary metabolite accumulation and ploidy determination following haplo-diploidization (Yanpaisan et al, 1999;Doležel et al, 2007a,b;Ochatt, 2008).…”