Flow-Cytometry in the Diagnosis of Diffuse Large B-Cell Lymphoma Based on Stomach Tissue Samples: A Case Report

Abstract

Morphology and immunohistochemistry on node, tissue, and bone marrow biopsies are frequently used in lymphoma diagnosis to characterize the stage and subtype of diseases. Multicolor flow cytometry technology is a novel technique for the analysis of immunological markers to identify lymphoma on fresh tissue when immunohistochemical staining is ambiguous. We report a case of a patient diagnosed with diffuse large B-cell lymphoma by flow cytometry on a stomach tissue biopsy.

An Overview of Advances in Rare Cancer Diagnosis and Treatment

“…Flow cytometry used to conduct early diagnosis of patients suspected with myeloid sarcoma (Armed Forces Medical College) [59], lung carcinoid (Precura Center) [60], and diffuse large B-cell lymphoma (Bach Mai Hospital) [61] • FISH technique for diagnosis of angiomatoid fibrous histiocytoma (Saitama Medical Center) [62] and renal cell carcinoma (Breach Candy Hospital Trust) [63], and other analysis that when combined can further improve the understanding of rare cancer etiology and diagnosis.…”

“…Cont. Myeloid sarcoma (Armed Forces Medical College)[59], lung carcinoid (Precura Center)[60], and diffuse large B-cell lymphoma (Bach Mai Hospital) were diagnosed by flow cytometry[61] • MRI, MR, and computed tomography were used for the visual examination of patients with undiagnosed basaloid carcinoma of the prostate (Royal Marsden Hospital)[69], malignant transformation of mature cystic teratoma (Nepal Cancer Hospital and Research Center)[58], squamous cell carcinoma of the prostate (Keio University Hospital and Qingdao Municipal Hospital)[52,57] • Genome sequencing analysis diagnosed and detected cancerous sequences in patients with ovarian immature teratomas (University of California, San Francisco Medical Center)[51] and squamous cell carcinoma of the prostate (Qingdao Municipal Hospital)[52] …”