Flow cytometry in diagnostic cytology

Analysis of Cerebrospinal Fluid Cells by Flow Cytometry and Immunocytochemistry in Inflammatory Central Nervous System Diseases: Comparison of Low‐ and High‐Density Cell Surface Antigen Expression

“…Similarly to a recent study, 23 we found that the examination of CSF cells by immunocytochemistry can give more reliable results as compared to flow cytometry, when analyzing antigens that are expressed at low density. This was most apparent when studying CSF with low cellularity, as from patients with chronic inflammatory diseases such as multiple sclerosis.…”

“…16 In a study of different body fluids using flow cytometry, it was concluded that this technique can be a useful adjunct to visual cytologic assessment of CSF. 23 Direct visualization of the cell morphology and staining pattern is helpful in order to avoid false-positive results due Fig. 1.…”

Evaluation of flow cytometric immunophenotyping and DNA analysis for detection of malignant cells in serosal cavity fluids

“…Adenocarcinomas were more likely to stain positively with B72.3, Ber-EP4, and CEA and negatively for vimentin. As FCM is a method that can evaluate a great number of cells in a short time with quantitative evaluation, and it could be complementary to the cytopathological examination, [33][34][35][36] we decided to study two of these markers (cytokeratin and Ber-EP4) in combination with CD45 and CD3 by multiparametric FCM which can detect and differentiate malignant cells. We found that Ber-EP4 immunophenotyping had the highest sensitivity and specificity in the detection of carcinoma cells in serous effusions and correlated with cytology in most of cases (73%).…”

“…37 One of FCM accepted applications is the measurement of DNA content, which allows the identification of cell populations with abnormal quantities of DNA (aneuploidy) and provides information on the cellular proliferate activity by analyzing the distribution of cells in their different phases of the cell cycle. 34,36 The analysis of the DNA aneuploidy through FCM has been used in an attempt to increase the accuracy of the analysis of effusions by cytology; it is able to identify abnormal cell populations not recognized by conventional cytopathological examination. 39,40 However, the greatest limitation to the widespread application of this method is the lack of concordance among the published studies.…”

Lymphoid and myeloid neoplasms involving cerebrospinal fluid: Comparison of morphologic examination and immunophenotyping by flow cytometry

“…Flow cytometry is a useful adjunct to cytologic examination because the quantitative phenotypic information it provides complements the morphologic information gained during morphologic examination. 20,30 It is also easily performed on aspirated lymphoid samples because the cells are already disaggregated. 21,22 Several comparative studies of cytospin preparations and flow cytometry have been reported, including lymph nodes, spleen, and extranodal sites.…”