Flow cytometry in diagnosis and management of large fetomaternal haemorrhage.

Johnson, Peter; Tait, Robert C.; Austin, E. B.; Shwe, K. H.; Lee, D.

doi:10.1136/jcp.48.11.1005

Cited by 47 publications

(45 citation statements)

References 9 publications

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“…A few clinical studies [3,12] have reported a good correlation between KBT and FC. In the present study, it was found that the volume of FMH estimated by KBT and FC methods in the post-delivery samples correlated well (r = 0.75; ICC ␣ = 0.73), whereas in the pre-delivery samples the correlation was poor (r = 0.08; ICC ␣ = 0.12).…”

Section: Discussionmentioning

confidence: 99%

“…However, it is often difficult to standardize. Further, it is laborious, difficult to reproduce and suffers from subjectivity and imprecision [3,4] . Flow cytometry (FC) for fetal cell detection is an evolving technology [3,5] .…”

Section: Introductionmentioning

confidence: 99%

“…Further, it is laborious, difficult to reproduce and suffers from subjectivity and imprecision [3,4] . Flow cytometry (FC) for fetal cell detection is an evolving technology [3,5] . The purpose of this work is to compare the accuracy of FC and KBT in the detection and quantification of FMH following delivery in Rhnegative non-immunized pregnancies.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Fetomaternal Hemorrhage by Flow Cytometry and Kleihauer-Betke Test in Rh-Negative Pregnancies

Savithrisowmya¹,

Singh

Kriplani³

et al. 2007

Gynecol Obstet Invest

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Objectives: To assess the efficacy of flow cytometry (FC) in the detection and quantification of fetomaternal hemorrhage (FMH) in comparison to the Kleihauer-Betke test (KBT). Methods: 25 unsensitized Rh-negative mothers who had delivered Rh-positive infants were included. Presence of FMH was determined by KBT and FC using FITC-labeled BRAD-3 antibodies. Results: FMH was detected in 19 (76%) patients by FC and 23 (92%) patients by KBT prior to delivery, and in 21 (84%) patients by FC and 23 (92%) patients by KBT after delivery. The mean volume of FMH in the post-delivery samples by KBT and FC were 0.34 ± 0.26 ml (range 0.05–1.2 ml) and 0.37 ± 0.57 ml (range 0.02–2.6 ml) respectively. The volume of post-delivery FMH estimated by KBT and FC correlated well (r = 0.75; ICC α = 0.73). A higher agreement between KBT and FC was seen in the 0.1–0.5 ml range (ĸ = 0.65; p < 0.01). Conclusions: Both manual KBT and FC using FITC-BRAD-3 antibodies show good sensitivity in detecting and quantifying fetal red cells. There is a good correlation between the methods in the 0.1- to 0.5-ml range of FMH.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of Fetomaternal Hemorrhage by Flow Cytometry and Kleihauer-Betke Test in Rh-Negative Pregnancies

Savithrisowmya¹,

Singh

Kriplani³

et al. 2007

Gynecol Obstet Invest

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“…Restricted tubular reabsorption may occur as a result of tubulus ischemic damage or an overload of the tubular reabsorption capacity. A decrease in the glomerular filtration rate to Ͻ70 mL/min will cause an overload of the tubular reabsorption capacity for ␣1M (10 ), and the ␣1M concentration in the plasma will increase with increasingly restricted filtration.…”

mentioning

confidence: 99%

Cell-free Fetal DNA in Maternal Circulation after Amniocentesis

et al. 2003

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“…in a patient with hereditary persistence of HbF [70]. Other investigators have reported the use of an indirect anti-D method for FMH quantitation [70][71][72][73][74]. In 1996, Lloyd-Evans et al [75] introduced an FC method using a directly labeled anti-D (i.e.…”

Section: Detection and Quantitation Of Red Blood Cell Antigensmentioning

confidence: 99%

Flow Cytofluorometric Analysis in Red Blood Cell Immunology

Arndt¹,

Garratty²

2004

Transfus Med Hemother

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Flow cytofluorometry (FC) is primarily used in the clinical laboratory for the analysis of white blood cells but also has applications in the study of red blood cell (RBC) immunology. When studying RBCs by FC it is important to avoid agglutination of the RBCs, which ironically is the endpoint for most immunohematologic assays. When studying low levels of RBC-bound antibody or RBC antigens with low copy number, background noise due to autofluorescence and nonspecific binding need to be taken into account. It is also important to ensure that reagents (e.g. antisera) used by FC have been standardized for use by FC. Applications of FC in RBC immunology include i) detection and quantitation of globulin (RBC-bound or free in serum), ii) detection and quantitation of RBC antigens, and iii) detection and quantitation of mixed cell populations. FC can be used to differentiate the amount of IgG on strongly IgG-coated RBCs, as well as to detect small amounts of RBC-bound IgG. FC has been used to quantitate antibody that is free in serum, e.g. anti-D in a pregnant woman or in immune globulin preparations. Many RBC antigens have been studied by FC to determine the number of antigen sites, the density distribution, and phenotype-related differences (e.g. zygosity). FC has been found to be very useful for studying mixed cell populations; applications include fetomaternal hemorrhage quantitation, determination of survival/ clearance of transfused RBCs, phenotyping of transfused patients’ RBCs, evaluation of bone marrow transplant engraftment, and study of RBC chimerism and mosaicism.

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Flow cytometry in diagnosis and management of large fetomaternal haemorrhage.

Cited by 47 publications

References 9 publications

Assessment of Fetomaternal Hemorrhage by Flow Cytometry and Kleihauer-Betke Test in Rh-Negative Pregnancies

Assessment of Fetomaternal Hemorrhage by Flow Cytometry and Kleihauer-Betke Test in Rh-Negative Pregnancies

Cell-free Fetal DNA in Maternal Circulation after Amniocentesis

Flow Cytofluorometric Analysis in Red Blood Cell Immunology

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