Flow cytometry for assessment of the tumor microenvironment in pediatric Hodgkin lymphoma

Henry, Meret; Buck, Steven; Savaşan, Süreyya

doi:10.1002/pbc.27307

Cited by 14 publications

(18 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, EBV+ cases with Th1 reaction did not show a better prognosis. In contrast, EBV+ pediatric patients exhibited a cytotoxic profile characterized by increased infiltration of CD8 + T cells and showed a better therapeutic response 8,73‐75 . However, a subset of EBV+ patients showed worse outcome with a high number of FOXP3+ cells, suggesting EBV modulates the immune escape strategies by enrichment of the TME with T reg cells and associated, immunosuppressive cytokines 76 .…”

Section: Tme Modulatorsmentioning

confidence: 99%

“…In contrast, EBV+ pediatric patients exhibited a cytotoxic profile characterized by increased infiltration of CD8 + T cells and showed a better therapeutic response. 8 , 73 , 74 , 75 However, a subset of EBV+ patients showed worse outcome with a high number of FOXP3+ cells, suggesting EBV modulates the immune escape strategies by enrichment of the TME with T reg cells and associated, immunosuppressive cytokines. 76 This outcome could be attributed to an insufficient intratumoral immune response against EBV in adult HL that fails to remove tumor cells.…”

Section: Tme Modulatorsmentioning

confidence: 99%

See 1 more Smart Citation

The circuitry of the tumor microenvironment in adult and pediatric Hodgkin lymphoma: cellular composition, cytokine profile, EBV, and exosomes

2020

View full text Add to dashboard Cite

Background: Classical Hodgkin lymphoma (cHL) is a unique lymphoid malignancy with a tumor microenvironment (TME) consisting of a small number of neoplastic—Hodgkin and Reed‐Sternberg (H‐RS) cells (<1%), surrounded by a large number of nonneoplastic infiltrating immune cells (>90%). The TME of cHL critically depends on immune cells to support tumor growth as H‐RS cells cannot survive and proliferate in isolation. Recent Findings: Programmed cell death protein 1 (PD‐1) ligand expressed on H‐RS cells inhibits the clearance of tumor by causing T‐cell exhaustion. Nivolumab and pembrolizumab, PD‐1 inhibitors, have been proven to be effective in treating adult and pediatric patients with R/R cHL. Tumor‐associated macrophages (TAMs) are a central component of TME and are known to cause poor prognosis in adult HL. However, the prognostic impact of CD68+ TAMs in pediatric HL remains ambiguous. EBV modulates the tumor milieu of HL and plays a strategic role in immune escape by enrichment of the TME with Treg cells and associated immunosuppressive cytokines in adult HL. In contrast, EBV+ pediatric patients have increased infiltration of CD8+ T‐cells and show a better therapeutic response suggesting viral‐related TME is distinct in childhood HL. The role of CASP3 in apoptosis of H‐RS cells and its correlation with response prediction in adult and pediatric HL suggest it may serve as a potential biomarker. In cHL, CD30, EBV, and NF‐κB signaling employ exosomes for cell–cell communication that triggers the migration capacity of fibroblasts, stimulate to produce proinflammatory cytokines, and help to create a tumor‐supportive microenvironment. Conclusion: The cHL microenvironment is distinct in adult and pediatric HL. Future studies are required to understand the role of interplay between H‐RS cells and EBV‐associated microenvironment and their clinical outcome. They may present novel therapeutic targets for the development of antilymphoma therapy.

show abstract

Section: Tme Modulatorsmentioning

confidence: 99%

Section: Tme Modulatorsmentioning

confidence: 99%

The circuitry of the tumor microenvironment in adult and pediatric Hodgkin lymphoma: cellular composition, cytokine profile, EBV, and exosomes

2020

View full text Add to dashboard Cite

show abstract

“…There was no correlation between the proportion of BM CD38/HLA-DR co-positive lymphocytes and stage or the presence of B symptoms. CD38/HLA-DR co-positive lymphocyte proportion inversely correlated with CD4/CD8 ratio in the BM as it was observed in the LN tissue 2. There were no significant differences between PB and BM flow cytometric profiles of lymphocyte, monocyte and myeloid cells overall (table 3).…”

Section: Resultsmentioning

confidence: 56%

“…An independent significant positive prognostic lymphocyte population, characterised by CD38/HLA-DR co-positive cells was lower in the BM compared with the LN tissue 2. Since LN tissue has much higher CD4-positive T cell presence, CD38/HLA-DR co-positive activated CD4-positive cells might play a different role than the same cells present in the BM.…”

Section: Discussionmentioning

confidence: 87%

See 1 more Smart Citation

Differing reflections of paediatric classical Hodgkin’s lymphoma on local and distant immunological microenvironments: a flow cytometric study

et al. 2019

Self Cite

View full text Add to dashboard Cite

AimsTo compare immunological microenvironments in local and distant lymphoid tissues in Hodgkin’s lymphoma (HL) in children.MethodsWe have analysed diagnostic bone marrow (BM) samples in 22 and corresponding involved lymph node (LN) in eight and peripheral blood (PB) in eight cases of HL by flow cytometry and sought correlations with clinical features retrospectively.ResultsWhile there were significant differences in lymphocyte compositions of BM and LN tissues, the distribution of lymphocyte subsets mimicked each other in BM and PB. CD8-positive cytotoxic T cells predominate the bone marrow in contrast to CD4-positive helper T cells in LN tissue with corresponding CD4/CD8 ratios (0.85 and 5.3, respectively; p=0.002). Additionally, T-large granular lymphocytes population was much higher in BM in comparison to LN tissue (10.5% vs 4.5%; p=0.036).ConclusionsLocal immunological microenvironment appears to be highly influenced by HL tumour cells and distant site lymphocyte composition reflects immune response to control the neoplastic process.

show abstract

References

2021

Flow Cytometry of Hematological Malignancies

View full text Add to dashboard Cite

Flow cytometry for assessment of the tumor microenvironment in pediatric Hodgkin lymphoma

Abstract: This study indicates that flow cytometry can be used to examine the tumor microenvironment in HL and that percentage of HLA-DR/CD38 copositive lymphocytes may be a biomarker for relapse and refractoriness in pediatric HL.

Cited by 14 publications

References 33 publications

The circuitry of the tumor microenvironment in adult and pediatric Hodgkin lymphoma: cellular composition, cytokine profile, EBV, and exosomes

The circuitry of the tumor microenvironment in adult and pediatric Hodgkin lymphoma: cellular composition, cytokine profile, EBV, and exosomes

Differing reflections of paediatric classical Hodgkin’s lymphoma on local and distant immunological microenvironments: a flow cytometric study

References

Contact Info

Product

Resources

About