Flow cytometry evaluation of in vitro susceptibility of bovine isolates of Tritrichomonas foetus to metronidazole

Rivero, María Belén; Luque, Melchor Emilio; Abdala, M E; Luna, Bruno Elías; Lullo, David Di; Carranza, Pedro Gabriel; Rivero, Fernando

doi:10.1016/j.vetpar.2019.02.004

Cited by 4 publications

(3 citation statements)

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“…Research based on ethnomedicine results in a more effective method for the selection of medicinal plants by reducing empiricism and increasing the probability of obtaining extracts and active ingredients for the production of medicines (Laxminarayan et In contrast to what was observed in this work, studies of susceptibility of T. foetus to metronidazole have shown a higher resistance of the protozoa under aerobic conditions than under anaerobic conditions (two to three orders of magnitude) (Rivero et al, 2019), which coincides with the mechanism of aerobic resistance in which oxygen interferes with the antimicrobial effect of metronidazole (Kulda, 1999). These results allow us to think that the mechanism of action of L. camara extract is different from that of metronidazole.…”

Section: Discussionmentioning

confidence: 59%

“…All assays were performed in triplicate. Live cells were counted every 24 h using ow cytometry and growth curves were determined according to Rivero et al (2019). Cell counts performed by ow cytometry were carried out on an Attune® acoustic focusing cytometer (Applied Biosystems, Thermo Fisher Scienti c).…”

Section: Determination Of Initial Inoculummentioning

confidence: 99%

“…Rivero et al (2019).2.3.3. Determination of 50% of the minimum inhibitory concentration (IC 50 ).The Minimum Inhibitory Concentration (MIC), the minimum concentration of a substance necessary to prevent microbial growth(Madigan et al, 2015), is an important parameter to de ne the in vitro susceptibility of a microorganism to a drug.…”

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confidence: 99%

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Evaluation of the susceptibility of Tritrichomonas foetus to extracts of Lantana camara (Verbenaceae) by flow cytometry

López

Luque²,

Rivero³

et al. 2023

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Bovine Trichomonosis (BT), a sexually transmitted disease endemic in countries with extensive cattle farming and natural service, is one of the most common causes of reproductive failure. 5-nitroimidazoles and their derivatives are used for its treatment, mainly metronidazole. The emergence of drug resistance mechanisms and treatment failures raise the need to investigate the effectiveness of new active compounds that contribute to parasite control. In this regard, extracts of Lantana camara (Verbenacea) have shown high biocidal potential against isolates of Trypanosoma cruzi and Leishmania braziliensis in vitro assays, although their effect on Tritrichomonas foetus has not been demonstrated yet. The available information on in vitro susceptibility of trichomonicidal drugs comes from the use of a diversity of methodologies and criteria, especially the observation of parasite motility under the optical microscope to assess their viability. Recently, in our laboratory, the use of flow cytometry has been described for the first time as a rapid and efficient method to evaluate the viability of T. foetus against metronidazole. The present study aimed to evaluate the cytostatic effect of L. camara extracts against T. foetus isolates by flow cytometry. Under aerobic conditions, IC50 values of 22.60 µg/mL were obtained on average. Under anaerobic conditions, the IC50 oscilated around 29.04 µg/mL. The results obtained allowed describing the susceptibility exhibited by these protozoa, being a valuable information for the development of potential BT treatments.

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Section: Discussionmentioning

confidence: 59%

Section: Determination Of Initial Inoculummentioning

confidence: 99%