Flow cytometry-evaluated platelet CD36 expression, reticulated platelets and platelet microparticles in essential thrombocythaemia and secondary thrombocytosis

Flow cytometry demonstrates differences in platelet reactivity and microparticle formation in subjects with thrombocytopenia or thrombocytosis due to primary haematological disorders

“…Increased microparticle counts in these subjects are therefore most likely to reflect the increased platelet count and not a hyperactivity of these platelets. This finding is in contrast to a previous study by MolesMoreau et al, whose work found that in comparison to normal controls, the microparticle/platelet ratio were significantly higher [17]. Trappenburg et al also found significantly increased platelet-derived microparticle counts in subjects with ET when compared to normal controls, but did not present their results in terms of the original platelet count [18].…”

Erythrocyte-derived microvesicles induce arterial spasms in JAK2V617F myeloproliferative neoplasm

“…The normalization of vascular reactivity induced by NAC could be explained by the glutathione inducer activity of NAC, but could also just be due to the potent antioxidant activity of this drug. microvesicles for both groups, which suggests that microvesicle composition, and not concentration, accounts for the observed vascular effect (27,28,(32)(33)(34)(35). We cannot rule out the possibility that JAK2 V617F erythrocytes themselves, in addition to microvesicles, also directly increase arterial contraction associated with MPNs.…”

Microparticle counts in platelet-rich and platelet-free plasma, effect of centrifugation and sample-processing protocols

“…Whole blood is the sample with the least manipulation for measuring microparticles, but has the disadvantage of large numbers of red cells that must be screened out of the data to collect a relatively small number of microparticle events [8,9,23]. Only a few studies have measured microparticles in whole blood; they reported about 6000/ml platelet-derived microparticles [8,9]. We evaluated PRP, not whole blood, as the best estimate of normal in-vivo microparticle numbers as the centrifugation needed to remove red cells (100g à 10 min ¼ 1000g min) was 30-fold less than that required to prepare platelet-poor plasma (30 000g min), and thus was unlikely to remove microparticles.…”