Flow Cytometry-Based Quantification of Cellular Au Nanoparticles

Park, Jonghoon; Ha, My Kieu; Yang, Nuri; Yoon, Tae Hyun

doi:10.1021/acs.analchem.6b04418

Cited by 64 publications

(58 citation statements)

References 32 publications

(60 reference statements)

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“…Apart from the differences in SSC and PI intensities, the FSC intensity of each population also displayed distinct features ( Figure 1b). FSC intensity is proportional to the size of cells, and changes in FSC intensity of treated cells in comparison to control cells reflect the swelling or shrinking of cells and may also provide us some hints about the toxicity of the treatment [16,24]. In the Ag 40 -treated sample, while the average FSC intensity of Type I cells was approximately 6 × 10 6 a.u.…”

Section: Single-cell Dose-response Analysis By Flow Cytometrymentioning

confidence: 97%

“…on average for all three populations. This is understandable because the scattering intensity is proportional to the particle size [5,16], so cells treated with Ag 80 NPs would have higher SSC intensity than those treated with Ag 40 NPs. Our previous study also reported that the agglomeration state of NPs can affect the amount of cellular NPs [5].…”

Section: Single-cell Dose-response Analysis By Flow Cytometrymentioning

confidence: 99%

“…For NPs that do not have fluorescence but have high scattering efficiency instead, the detection of their cellular association using the side-scattered (SSC) intensity is more appropriate, as the SSC intensity is related to the inner granularity of cells [13,14]. Our recent studies have introduced a quantitative approach to estimate the cellular content of non-fluorescent NPs by calibrating the SSC intensity in FCM with parallel quantification by ICP-MS [15,16].…”

Section: Introductionmentioning

confidence: 99%

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Heterogeneity in Biodistribution and Cytotoxicity of Silver Nanoparticles in Pulmonary Adenocarcinoma Human Cells

Chung

Yoon

2019

Nanomaterials

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Cellular association of nanoparticles (NPs) and their resultant cytotoxicity are heterogeneous in nature and can be influenced by the variances in NPs’ properties, cell types, and status. However, conventional in vitro assays typically consider the administered NP dose and the averaged cellular responses based on the assumption of a uniform distribution of monodisperse NPs in homogeneous cells, which might be insufficient to describe the complex nature of cell–NP interactions. Here, using flow cytometry, we report observations of the heterogeneity in the cellular association of silver nanoparticles (AgNPs) in A549 cells, which resulted in distinct dose-response relationships and cytotoxicity. Type I and Type II cells were moderately associated with AgNPs but as the cellular AgNP dose increased, Type I cells remained viable while Type II cells became less viable. Type III cells did not have high affinity with AgNPs but were, however, the least viable. Transmission electron microscopic images revealed that the biodistribution and the released Ag+ ions contributed to the distinct toxic effects of AgNPs in different populations. This single-cell dose-response analysis approach enabled the examination of how differently individual cells responded to different cellular NP doses and provided insights into nanotoxicity pathways at a single-cell level.

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Section: Single-cell Dose-response Analysis By Flow Cytometrymentioning

confidence: 97%

Section: Single-cell Dose-response Analysis By Flow Cytometrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Heterogeneity in Biodistribution and Cytotoxicity of Silver Nanoparticles in Pulmonary Adenocarcinoma Human Cells

Chung

Yoon

2019

Nanomaterials

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“…Recent reports showed that the measured side scattering (SSC) intensities while using the flow cytometry method are linearly related to the cellular association of the GNPs as well as the core sizes of the GNPs. 41 In comparisons between inductively coupled plasma mass spectrometry and flow cytometry results on human cells, researchers found linear correlations between the cellular GNPs and the SSC intensities as measured by flow cytometry and used them to estimate the amounts of GNPs associated with cells. Using this validated technique, comparisons with the SSC intensities of glioma cells exposed to four different types of GNPs ( Figure S4) were performed.…”

Section: Figurementioning

confidence: 99%

<p>Gold quantum dots impair the tumorigenic potential of glioma stem-like cells via β-catenin downregulation in vitro</p>

Wahab¹,

Kaushik²,

Khan³

et al. 2019

IJN

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Background: Over the past several decades, the incidence of solid cancers has rapidly increased worldwide. Successful removal of tumor-initiating cells within tumors is essential in the field of cancer therapeutics to improve patient disease-free survival rates. The biocompatible multivarient-sized gold nanoparticles (MVS-GNPs) from quantum dots (QDs, ,10 nm) to nanosized (up to 50 nm) particles have vast applications in various biomedical areas including cancer treatment. The role of MVS-GNPs for inhibition of tumorigenic potential and stemness of glioma was investigated in this study. Methods: Herein, MVS-GNPs synthesized and characterized by means of X-ray diffraction pattern (XRD) and transmission electron microscopy (TEM) techniques. Afterwards, interaction of these GNPs with glioma stem-cell like cells along with cancer cells were evaluated by MTT, cell motility, self-renewal assays and biostatistics was also applied. Results: Among these GNPs, G-QDs contributed to reduce metastatic events and spheroid cell growth, potentially blocking the self-renewal ability of these cells. This study also uncovers the previously unknown role of the inhibition of CTNNB1 signaling as a novel candidate to decrease the tumorigenesis of glioma spheroids and subsequent spheroid growth. The accurate and precise biostatistics results were obtained at quantify level. Conclusion: In summary, G-QDs may exhibit possible contribution on suppressing the growth of tumor-initiating cells. These data reveal a unique therapeutic approach for the elimination of residual resistant stem-like cells during cancer treatment.

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“…Moreover, due to the cross-talk and autofluorescence issues of the fluorophores, it fails to estimate the number of cell-associated NPs quantitatively (Orecchioni et al 2017). Flow cytometry (FCM) is another technique that can simultaneously monitor cellular NP uptake and cellular responses by measuring the light-scattering and fluorescence-labeling intensities, respectively (Park et al 2017, Ha et al 2018). However, for those cells that have wide heterogeneity (e.g., immune cells), FCM is inadequate in providing simultaneous analyses of cell type, activation status, and elevation/depletion of secreted molecules due to its limited number of fluorescence detection channels.…”

Section: Introductionmentioning

confidence: 99%

Mass Cytometry Study on the Heterogeneity in Cellular Association and Cytotoxicity of Silver Nanoparticles in Human Immune Cells

Choi

Gerelkhuu

et al. 2019

Preprint

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There have been many reports about the adverse effects of nanoparticles (NPs) on the environment and human health. Conventional toxicity assessments of NPs frequently assume uniform distribution of monodisperse NPs in homogeneous cell populations, and provide information on the relationships between the administered dose of NPs and cellular responses averaged for a large number of cells. They may have limitations in describing the wide heterogeneity of cell-NP interactions, caused by cell-to-cell and NPto-NP variances. To achieve more detailed insight into the heterogeneity of cell-NP interactions, it is essential to understand the cellular association and adverse effects of NPs at single-cell level. In this study, we applied mass cytometry to investigate the interactions between silver nanoparticles (AgNPs) and primary human immune cells.High dimensionality of mass cytometry allowed us to identify various immune cell types 2 and observe the cellular association and toxicity of AgNPs in each population. Our findings showed that AgNPs had higher affinity with phagocytic cells like monocytes and dendritic cells and caused more severe toxic effects than with T cells, B cells and NK cells. Multi-element detection capability of mass cytometry also enabled us to simultaneously monitor cellular AgNP dose and intracellular signaling of individual cells, and subsequently investigate the dose-response relationships of each immune population at single-cell level, which are often hidden in conventional toxicity assays at bulk-cell level. Our study will assist future development of single-cell dose-response models for various NPs and will provide key information for the safe use of nanomaterials for biomedical applications.

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Flow Cytometry-Based Quantification of Cellular Au Nanoparticles

Cited by 64 publications

References 32 publications

Heterogeneity in Biodistribution and Cytotoxicity of Silver Nanoparticles in Pulmonary Adenocarcinoma Human Cells

Heterogeneity in Biodistribution and Cytotoxicity of Silver Nanoparticles in Pulmonary Adenocarcinoma Human Cells

<p>Gold quantum dots impair the tumorigenic potential of glioma stem-like cells via β-catenin downregulation in vitro</p>

Mass Cytometry Study on the Heterogeneity in Cellular Association and Cytotoxicity of Silver Nanoparticles in Human Immune Cells

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Flow Cytometry-Based Quantification of Cellular Au Nanoparticles

Cited by 64 publications

References 32 publications

Heterogeneity in Biodistribution and Cytotoxicity of Silver Nanoparticles in Pulmonary Adenocarcinoma Human Cells

Heterogeneity in Biodistribution and Cytotoxicity of Silver Nanoparticles in Pulmonary Adenocarcinoma Human Cells

<p>Gold quantum dots impair the tumorigenic potential of glioma stem-like cells via &beta;-catenin downregulation in vitro</p>

Mass Cytometry Study on the Heterogeneity in Cellular Association and Cytotoxicity of Silver Nanoparticles in Human Immune Cells

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<p>Gold quantum dots impair the tumorigenic potential of glioma stem-like cells via β-catenin downregulation in vitro</p>