Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells

Abstract

Adipose tissue dysfunction is typically seen in metabolic diseases, particularly obesity and diabetes. White adipocytes store fat while brown adipocyte dissipates it via thermogenesis. In addition, beige adipocytes develop in white fat depots in response to stimulation of β-adrenergic pathways. It appears that the three types of adipocytes-white, brown, and beige-can be formed de novo from stem/precursor cells or via transdifferentiation. Identifying the presumptive progenitors that harbor capacity to differentiate to these distinct adipocyte cell types will enable their functional characterization. Moreover, the presence or absence of white/brown/beige adipocytes is correlated with metabolic dysfunction making their study of medical relevance. Robust, reliable, and reproducible methods of identification and isolation of adipocyte progenitors will stimulate further detailed understanding of white, brown, and beige adipogenesis.

Single xenotransplant of rat brown adipose tissue prolonged the ovarian lifespan of aging mice by improving follicle survival

“…In the present study, we did not include ADSCs; we removed and xenotransplanted only the BAT and obtained satisfying results. This might be explained by the fact that BAT contains intrinsic ADSCs that could function to reduce acute rejection, the ROS levels, and the inflammatory response as well as, or much better than extrinsically cultured ADSCs (Di Franco et al, 2014; Tran & Kahn, 2010; Wankhade & Rane, 2017). Alternatively, BATs could secrete an array of known or unknown brown adipokines (the so‐called brown adipokines or batokines), such as IL‐6, adiponectin, HGF, BFGF, fibroblast growth factor 21, and neuregulin 4, to counteract these detrimental reactions (Ding et al, 2018; Villarroya et al, 2017, 2013; Wang et al, 2015).…”

Adipocyte-Based Cell Therapy in Oncology: The Role of Cancer-Associated Adipocytes and Their Reinterpretation as Delivery Platforms

“…Both of these tests offer substantial information for developing such cell-based delivery systems. Furthermore, immunophenotipation at this step can reveal some significant changes concerning the cluster of differentiation panel [182]. A different assessment that can be performed at this step is called LIVE/DEAD staining (microscopy technique) [183].…”