Flow cytometry assessment of bacterioplankton in tropical marine environments

Andrade, Luciana Segura de; Gonzalez, Alessandra M.; Araújo, Fábio Vieira de; Paranhos, Rodolfo

doi:10.1016/j.mimet.2003.08.002

Cited by 62 publications

(49 citation statements)

References 34 publications

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“…The occurence of different bacterial sub-groups was also described in others aquatic environments (1,17). LNA bacteria were characterized by low size cells with low nucleic acid content.…”

Section: Resultsmentioning

confidence: 61%

Heterotrophic bacteria abundances in Rodrigo de Freitas Lagoon (Rio de Janeiro, Brazil)

Gonzalez¹,

Paranhos²,

Lutterbach³

2006

Braz. J. Microbiol.

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The Rodrigo de Freitas Lagoon (RJ, Brazil) is an important coastal ecosystem that has been submitted to an accelerated degradation process. The aim of this work was to determine the abundance and the spatial distribution of total heterotrophic (by flow cytometry) and cultivated bacteria ("pour plate" method on R2A agar). Another objective was to evaluate the lagoon's influence on water quality of Ipanema and Leblon beaches. Physical and chemical data were acquired too. Sub-superficial water samples were taken monthly,

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“…The occurence of different bacterial sub-groups was also described in others aquatic environments (1,17). LNA bacteria were characterized by low size cells with low nucleic acid content.…”

Section: Resultsmentioning

confidence: 61%

Heterotrophic bacteria abundances in Rodrigo de Freitas Lagoon (Rio de Janeiro, Brazil)

Gonzalez¹,

Paranhos²,

Lutterbach³

2006

Braz. J. Microbiol.

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“…Areas of severe sewage contamination were identified by higher concentrations of coprostanol (Carreira et al 2002). Bacterial activity reaches up to 7.35 μg C L -1 h -1 in the inner part of the bay where tidal circulation is restricted (Andrade et al 2003). However, Guanabara Bay is considered one of the most productive marine ecosystems in the world, with carbon assimilation values ranging between 800 and 3,600 mg.C.day -1 and a mean net primary production of 0.17 mol.C.m -2 .…”

Section: Study Areamentioning

confidence: 99%

The relationship between sandy beach nematodes and environmental characteristics in two Brazilian sandy beaches (Guanabara Bay, Rio de Janeiro)

Maria

Paiva

Vanreusel

et al. 2013

An. Acad. Bras. Ciênc.

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We investigated if the differences in density and nematode communities of intertidal sediments from two Brazilian sheltered sandy beaches were related to environmental characteristics. The upper tide level (UTL) and the low tide level (LTL) of both beaches were surveyed in January (austral summer) and June 2001 (austral winter) during low-spring tides, by collecting samples of nematodes and sediments. Differences in density between beaches, tidal level and seasons, and nematode community structure were investigated. Sediments from both beaches were composed of medium to very coarse sand. The highest nematode densities were found at the UTL, and significant differences between beaches, tidal levels and months were found. A total of 54 genera were found and the genera composition on both sheltered beaches was similar to other exposed worldwide sandy beaches. The density and structure of the nematode community at both beaches clearly varied along the spatial and temporal scales. Gravel percentage was the most important variable explaining the spatial distribution of the nematodes, determining the four sub-communities; this suggests that the sediment characteristics influence the nematode community, rather than physical hydrodynamic forces. Temperature and salinity were suggested to be important variables affecting the temporal variation.

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“…The introduction of flow cytometry made bacteria counts faster, easier, and more precise, providing feasibility for experiments involving a high number of counting samples to be processed (22). Also cytometric counts revealed the heterogeneity and differences in bacterioplankton populations allowing the distinction of two general groups: bacteria with high apparent nucleic acid content (HNA) and bacteria with low apparent nucleic acid content (LNA) (1,14,22,26,30,45). The HNA bacteria were considered the most active members of a given community while LNA were regarded as inactive, dead or dormant cells (17,26,27).…”

Section: Introductionmentioning

confidence: 99%

“…HNA and LNA were also considered to be members of the same phylotypes (11,38). However some have demonstrated that LNA are different phylotypes from HNA cells (12,31,45) and that indeed LNA can be responsible for a significant portion of total heterotrophic metabolism (1,22,31,34,45), all observations from oligotrophic systems as open oceans.…”

Section: Introductionmentioning

confidence: 99%

Distribution of HNA and LNA bacterial groups in the Southwest Atlantic Ocean

Andrade¹,

Gonzalez²,

Rezende³

et al. 2007

Braz. J. Microbiol.

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Bacterioplankton was studied in a large area of Southwest Atlantic Ocean between 13 and 25ºS and 28 and 42ºW. Samples were collected in 108 stations at 20 m depth. Bacteria were enumerated by flow cytometry after nucleic acid staining with syto13 and two subgroups were differentiated: low nucleic acid content (LNA) and high nucleic acid content (HNA) bacteria. Total bacterial numbers varied from 0.37 to 5.53 10 5 cells mL -1. HNA cells represented 15 to 70% of the total number while LNA cells represented 30 to 85%. Heterotrophic bacterial production was determined by incorporation of tritiated leucine and ranged from 2.7 to 171.07 ng C L -1 h -1. No significant correlation was found between abundance and production. Nevertheless with support of multivariate analysis between bacterial abundance, bacterial production, chlorophyll a and other oceanographic data the distribution of the groups in two different oceanic provinces could be explained by nutrient availability. HNA bacteria accounted for the high percentage of cells found in the area north of 19ºS, linked to higher temperature waters and riverine nutrients inputs. LNA bacteria were the dominant cells south of this latitude and were correlated to the higher values of nitrate found for the same area.

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Flow cytometry assessment of bacterioplankton in tropical marine environments

Cited by 62 publications

References 34 publications

Heterotrophic bacteria abundances in Rodrigo de Freitas Lagoon (Rio de Janeiro, Brazil)

Heterotrophic bacteria abundances in Rodrigo de Freitas Lagoon (Rio de Janeiro, Brazil)

The relationship between sandy beach nematodes and environmental characteristics in two Brazilian sandy beaches (Guanabara Bay, Rio de Janeiro)

Distribution of HNA and LNA bacterial groups in the Southwest Atlantic Ocean

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