Flow-cytometry applications in plant breeding

Ciprian-Salcedo, Gean Carlo; Jimenez-Davalos, Jorge; Zolla, Gastón

doi:10.15381/rpb.v27i1.17583

Cited by 4 publications

(5 citation statements)

References 37 publications

(44 reference statements)

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“…Nowadays, the main method used for this purpose is flow cytometry [8,29], which increases the efficiency of DNA ploidy level determination and nuclear genome size measurement. With flow cytometry large numbers of individuals are quickly evaluated in only one day using a quantitative, rapid, reliable and reproducible method [11]. This powerful screening tool for ploidy level has been very useful in forage breeding programs.…”

Section: Production Of Polyploid Plantsmentioning

confidence: 99%

“…Natural and induced polyploid have been widely investigated in crops, trees, ornamentals, forages and medicinal plants [10]. This literature search and synthesis were pursued to address 21st century challenges related to continuous population growth, as well as the global climate change [11]. Forages are widely exploited for their vegetative biomass that may be used as animal feed or for biofuel [12].…”

Section: Introductionmentioning

confidence: 99%

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Induced Polyploidy: A Tool for Forage Species Improvement

et al. 2021

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Polyploidy means having more than two basic sets of chromosomes. Polyploid plants may be artificially obtained through chemical, physical and biological (2n gametes) methods. This approach allows an increased gene scope and expression, thus resulting in phenotypic changes such as yield and product quality. Nonetheless, breeding new cultivars through induced polyploidy should overcome deleterious effects that are partly contributed by genome and epigenome instability after polyploidization. Furthermore, shortening the time required from early chromosome set doubling to the final selection of high yielding superior polyploids is a must. Despite these hurdles, plant breeders have successfully obtained polyploid bred-germplasm in broad range of forages after optimizing methods, concentration and time, particularly when using colchicine. These experimental polyploids are a valuable tool for understanding gene expression, which seems to be driven by dosage dependent gene expression, altered gene regulation and epigenetic changes. Isozymes and DNA-based markers facilitated the identification of rare alleles for particular loci when compared with diploids, and also explained their heterozygosity, phenotypic plasticity and adaptability to diverse environments. Experimentally induced polyploid germplasm could enhance fresh herbage yield and quality, e.g., leaf protein content, leaf total soluble solids, water soluble carbohydrates and sucrose content. Offspring of experimentally obtained hybrids should undergo selection for several generations to improve their performance and stability.

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Section: Production Of Polyploid Plantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Induced Polyploidy: A Tool for Forage Species Improvement

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Nowadays, the main used method for these purposes is the flow cytometry [8,29] that increases the efficiency of the DNA ploidy level determination and nuclear genome size measurement. From flow cytometry, large numbers of individuals are quickly evaluated in only one day in a quantitative, thus being rapid, reliable and reproducible [11]. This powerful screening tool for ploidy level has been very useful in forage breeding programs.…”

Section: Production Of Polyploid Plantsmentioning

confidence: 99%

“…Natural and induced polyploid have been widely investigated in crops, fruits, trees, ornamentals, forages and medicinal plants [10]. This research was pursued to address 21 st century challenges related to continuous population growth, as well as the global climate change [11].…”

Section: Introductionmentioning

confidence: 99%

Scope of Whole Genome Duplication in Forages: A Plant Breeder Perspective

Rauf¹,

Ortíz²,

Malinowski³

et al. 2021

Preprint

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Polyploidy is a condition of having more than two sets of chromosomes. Polyploid plants may be artificially obtained through chemical, physical and biological methods (2n gametes). It is considered an interesting approach due to increased genescope and expression, thus resulting in phenotypic changes, such as yield and product quality. Nonetheless, breeding new cultivars through induced polyploidy should overcome deleterious effects partly contributed by genome and epigenome instability after polyploidization. Furthermore, shortening the time required from early chromosome set doubling to the final selection of high yielding superior polyploids is a must. Despite these hurdles, plant breeders have successfully obtained polyploid bred-germplasm in broad range of forages from optimized application methods, concentration and time mainly using colchicine. These experimental polyploids proved to be a valuable tool for understanding gene expression that is driven by dosage dependent gene expression, altered gene regulation and epigenetic changes. Isozymes and DNA-based markers aided on the identification of rare alleles for particular loci when compared with diploids, which may explain their heterozygosity, phenotypic plasticity and adaptability to diverse environments. It has also been observed that experimentally induced polyploid germplasm could enhance fresh herbage yield and quality, e.g. leaf protein content, leaf total soluble solids, water soluble carbohydrates and sucrose content. Offspring of experimentally obtained hybrids underwent selection for several generations to improve their performance and stability.

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“…It is also useful for determining the genetic characterization of germplasm and plant identification because of these characteristics (Jedrzejczyk and Rewers 2020). Flow cytometry is a quick and easy way to estimate nuclear DNA content, making it a powerful tool for accelerating selection processes in plant breeding and providing critical data for sequencing projects (Ciprian-Salcedo et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Nuclear DNA content and comparative FISH mapping of the 5s and 45s rDNA in wild and cultivated populations of Physalis peruviana L.

et al. 2023

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Physalis peruviana L. often known as goldenberry, has increased its commercial growth in the international market in recent years due to its nutritional value and antioxidant potential. This situation has enabled countries such as Peru to increase their production in order to meet the global demand. However, investigations about the genetic diversity of cultivated and wild populations of goldenberry are still in their early stages. FISH mapping of 5s and 45s rDNA loci and flow cytometry estimation of nuclear DNA content were used to assess genetic differences between wild and cultivated goldenberry populations from Ayacucho and Cajamarca. The majority of metaphases had six 5s rDNA sites for all populations and two and four 45s rDNA sites for the cultivated and wild populations, respectively. We were able to characterize nine different types of chromosomes based on their morphology, fluorescence, rDNA location, and conservation across populations by analyzing the chromosomes that contained rDNA. Furthermore, cultivated populations had more nuclear DNA (13.262±0.087 pg) than wild populations (12.955±0.086 pg). The results show genetic differences between wild and cultivated populations of goldenberry at molecular cytogenetic level as well as in genome size. These findings establish a precedent for future cytogenetic and genomic studies in goldenberry populations, enabling future breeding programs.

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Flow-cytometry applications in plant breeding

Cited by 4 publications

References 37 publications

Induced Polyploidy: A Tool for Forage Species Improvement

Induced Polyploidy: A Tool for Forage Species Improvement

Scope of Whole Genome Duplication in Forages: A Plant Breeder Perspective

Nuclear DNA content and comparative FISH mapping of the 5s and 45s rDNA in wild and cultivated populations of Physalis peruviana L.

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