Flow Cytometry Analysis of Marine Picoplankton

Marie, Dominique; Simon, Nathalie; Guillou, Laure; Partensky, Frédéric; Vaulot, Daniel

doi:10.1007/978-3-642-57049-0_34

Cited by 120 publications

(89 citation statements)

References 15 publications

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“…Counts were performed on a FACS-Calibur flow cytometer (Becton Dickinson, San Jose, CA, USA), equipped with a 488 nm wavelength, 15 mW Argon laser. Separation of different autotrophic populations was based on their scattering and fluorescence signals according to Marie et al (2000). Synechococcus spp.…”

Section: Flow Cytometrymentioning

confidence: 99%

Assessing the role of dust deposition on phytoplankton ecophysiology and succession in a low-nutrient low-chlorophyll ecosystem: a mesocosm experiment in the Mediterranean Sea

Giovagnetti

Brunet²,

Conversano³

et al. 2013

Biogeosciences

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In this study, we investigate the response of the phytoplankton community, with emphasis on ecophysiology and succession, after two experimental additions of Saharan dust in the surface water layer of a low-nutrient low-chlorophyll ecosystem in the Mediterranean Sea. Three mesocosms were amended with evapocondensed dust to simulate realistic Saharan dust events, while three additional mesocosms were kept unamended and served as controls. The experiment consisted in two consecutive dust additions and samples were daily collected at different depths (−0.1, −5 and −10 m) during one week, starting before each addition occurred. Data concerning HPLC pigment analysis on two size classes (< 3 and > 3 μm), electron transport rate (ETR) vs. irradiance curves, non-photochemical fluorescence quenching (NPQ) and phytoplankton cell abundance (measured by flow cytometry), are presented and discussed in this paper. Results show that picophytoplankton mainly respond to the first dust addition, while the second addition leads to an increase of both pico- and nano-/microphytoplankton. Ecophysiological changes in the phytoplankton community occur, with NPQ and pigment concentration per cell increasing after dust additions. While biomass increases after pulses of new nutrients, ETR does not greatly vary between dust-amended and control conditions, in relation with ecophysiological changes within the phytoplankton community, such as the increase in NPQ and pigment cellular concentration. A quantitative assessment and parameterisation of the onset of a phytoplankton bloom in a nutrient-limited ecosystem is attempted on the basis of the increase in phytoplankton biomass observed during the experiment. The results of this study are discussed focusing on the adaptation of picophytoplankton to nutrient limitation in the surface water layer, as well as on size-dependent competition ability in phytoplankton

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Section: Flow Cytometrymentioning

confidence: 99%

Assessing the role of dust deposition on phytoplankton ecophysiology and succession in a low-nutrient low-chlorophyll ecosystem: a mesocosm experiment in the Mediterranean Sea

Giovagnetti

Brunet²,

Conversano³

et al. 2013

Biogeosciences

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“…The abundances of total heterotrophic prokaryotes (including AAP bacteria) and autotrophic prokaryotes (Synechococcus and Prochlorococcus) were measured by flow cytometry using a FACSCalibur flow cytometer (Becton Dickinson, San Jose, CA) according to Marie et al (2002). The bacterial production was determined by [ 3 H]-leucine incorporation applying the centrifugation method (Smith and Azam, 1992) and is detailed in Van Wambeke et al (2010).…”

Section: Bacterioplankton Abundance and Heterotrophic Prokaryotic Promentioning

confidence: 99%

Ecology of aerobic anoxygenic phototrophic bacteria along an oligotrophic gradient in the Mediterranean Sea

et al. 2011

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Abstract. Aerobic anoxygenic phototrophic (AAP) bacteria are photoheterotrophic prokaryotes able to use both light and organic substrates for energy production. They are widely distributed in coastal and oceanic environments and may contribute significantly to the carbon cycle in the upper ocean. To better understand questions regarding links between the ecology of these photoheterotrophic bacteria and the trophic status of water masses, we examined their horizontal and vertical distribution and the effects of nutrient additions on their growth along an oligotrophic gradient in the Mediterranean Sea. Concentrations of bacteriochlorophyll-a (BChl-a) and AAP bacterial abundance decreased from the western to the eastern basin of the Mediterranean Sea and were linked with concentrations of chlorophyll-a, nutrient and dissolved organic carbon. Inorganic nutrient and glucose additions to surface seawater samples along the oligotrophic gradient revealed that AAP bacteria were nitrogen-and carbon-limited in the ultraoligotrophic eastern basin. The intensity of the Correspondence to: D. Lamy (lamyd2@univie.ac.at) AAP bacterial growth response generally differed from that of the total bacterial growth response. BChl-a quota of AAP bacterial communities was significantly higher in the eastern basin than in the western basin, suggesting that reliance on phototrophy varied along the oligotrophic gradient and that nutrient and/or carbon limitation favors BChl-a synthesis.

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“…Based on a plot of green versus red fluorescence we distinguished photosynthetic from non-photosynthetic prokayotes. Enumeration of autotrophic cells was performed according to Marie et al (2000). Synechococcus spp.…”

Section: Pigment Analysismentioning

confidence: 99%

Biochemical characteristics and bacterial community structure of the sea surface microlayer in the South Pacific Ocean

et al. 2008

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Abstract. The chemical and biological characteristics of the surface microlayer were determined during a transect across the South Pacific Ocean in October-December 2004. Concentrations of particulate organic carbon (1.3 to 7.6-fold) and nitrogen (1.4 to 7-fold), and POC:PON ratios were consistently higher in the surface microlayer as compared to surface waters (5 m). The large variability in particulate organic matter enrichment was negatively correlated to wind speed. No enhanced concentrations of dissolved organic carbon were detectable in the surface microlayer as compared to 5 m, but chromophoric dissolved organic matter was markedly enriched (by 2 to 4-fold) at all sites. Based on pigment analysis and cell counts, no consistent enrichment of any of the major components of the autotrophic and heterotrophic microbial community was detectable. CE-SSCP fingerprints and CARD FISH revealed that the bacterial communities present in the surface microlayer had close similarity (>76%) to those in surface waters. By contrast, bacterial heterotrophic production ( 3 H-leucine incorporation) was consistently lower in the surface microlayer than in surface waters. By applying CARD-FISH and microautoradiography, we observed that Bacteroidetes and Gammaproteobacteria dominated leucine uptake in the surface microlayer, while in surface waters Bacteroidetes and Alphaproteobacteria were the major groups accounting for leucine incorporation. Our results demonstrate that the microbial community in the surface microlayer closely resembles that of the surface Correspondence to: I. Obernosterer (ingrid.obernosterer@obs-banyuls.fr)waters of the open ocean. Even a short residence in the surface microlayer influences leucine incorporation by different bacterial groups, probably as a response to the differences in the physical and chemical nature of the two layers.

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Flow Cytometry Analysis of Marine Picoplankton

Cited by 120 publications

References 15 publications

Assessing the role of dust deposition on phytoplankton ecophysiology and succession in a low-nutrient low-chlorophyll ecosystem: a mesocosm experiment in the Mediterranean Sea

Assessing the role of dust deposition on phytoplankton ecophysiology and succession in a low-nutrient low-chlorophyll ecosystem: a mesocosm experiment in the Mediterranean Sea

Ecology of aerobic anoxygenic phototrophic bacteria along an oligotrophic gradient in the Mediterranean Sea

Biochemical characteristics and bacterial community structure of the sea surface microlayer in the South Pacific Ocean

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