Flow cytometry analysis of anti-polyethylene glycol antibodies in human plasma

Fang, Jia‐Long; Beland, Frederick A.; Tang, Yangshun; Roffler, Steve R.

doi:10.1016/j.toxrep.2020.12.022

Cited by 25 publications

(14 citation statements)

References 30 publications

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“…While the FDA has approved the clinical use of nanoparticles with polymer coatings, such as poly(ethylene glycol) (PEG) and dextran, these coating agents have been reported in some cases to impact nanomedicine safety and efficacy adversely. − These reports have raised growing clinical concern about anti-PEG immunogenicity, which may be amplified by the widespread use of PEG in cosmetics, health care products, and over-the-counter medications. , Anti-PEG antibodies can bind to PEGylated nanoparticles, which may induce undesired immune responses, including premature clearance of nanomedicines, allergic reactions, and anaphylaxis. ,− There is a need to investigate alternative nanoparticle surface modifications that can address the shortcomings of PEGylated nanomedicines …”

Section: Introductionmentioning

confidence: 99%

Nanoparticle Surface Engineering with Heparosan Polysaccharide Reduces Serum Protein Adsorption and Enhances Cellular Uptake

et al. 2022

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Nanoparticle modification with poly(ethylene glycol) (PEG) is a widely used surface engineering strategy in nanomedicine. However, since the artificial PEG polymer may adversely impact nanomedicine safety and efficacy, alternative surface modifications are needed. Here, we explored the “self” polysaccharide heparosan (HEP) to prepare colloidally stable HEP-coated nanoparticles, including gold and silver nanoparticles and liposomes. We found that the HEP-coating reduced the nanoparticle protein corona formation as efficiently as PEG coatings upon serum incubation. Liquid chromatography–mass spectrometry revealed the protein corona profiles. Heparosan-coated nanoparticles exhibited up to 230-fold higher uptake in certain innate immune cells, but not in other tested cell types, than PEGylated nanoparticles. No noticeable cytotoxicity was observed. Serum proteins did not mediate the high cell uptake of HEP-coated nanoparticles. Our work suggests that HEP polymers may be an effective surface modification technology for nanomedicines to safely and efficiently target certain innate immune cells.

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Section: Introductionmentioning

confidence: 99%

Nanoparticle Surface Engineering with Heparosan Polysaccharide Reduces Serum Protein Adsorption and Enhances Cellular Uptake

et al. 2022

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“…The evidence that approximately one-third of those who experienced anaphylaxis after vaccination had previous history of allergic or anaphylactic reactions suggests that anti-PEG antibodies formed after exposure to PEGylated compounds may play a role 9 . Indeed, recent research on plasma samples from hospitals or service companies based in the United States found that 65-76% of the general population had anti-PEG antibodies 10,11 at an average concentration of 52 ng ml −1 (ref. 10 ).…”

mentioning

confidence: 99%

The role and impact of polyethylene glycol on anaphylactic reactions to COVID-19 nano-vaccines

et al. 2021

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“…On a technical level, it is important to note the possible interference of bovine serum albumin and Tween 20, often used in ELISA; skimmed milk and an alternative detergent would probably be more appropriate reagents (35). Flow cytometric methods have also been described to assay anti-PEG IgE (36). Interestingly, Zhou et al (21) found anti-PEG IgE and IgG in patients who had an anaphylactic reaction to products for colonoscopy preparation containing PEG 3350.…”

Section: Biological Evaluation Of Covid19 Vaccine-induced Allergy Ant...mentioning

confidence: 99%

Immediate hypersensitivity to COVID-19 vaccines: Focus on biological diagnosis

Nicaise-Roland

Granger²,

Soria³

et al. 2022

Front. Allergy

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Soon after the release of the new anti-COVID mRNA vaccines, reports came in from the US and the UK of anaphylactic reactions. Fueled by the necessary caution toward these new vaccine platforms, these reports had a great impact and were largely commented upon in the scientific literature and global media. The current estimated frequency is of 5 cases per million doses. Very little biological data are presented in the literature to support the anaphylaxis diagnosis in these patients in addition to skin tests. Allergic reactions to vaccines are rare and mostly due to vaccine excipient. Therefore, the poly-ethylene-glycol (PEG) present in both mRNA formulation, and already known to be immunogenic, was soon suspected to be the potential culprit. Several hypersensitivity mechanisms to PEG or to other vaccine components can be suspected, even if the classical IgE-dependent anaphylaxis seems to be one of the most plausible candidates. In the early 2022, the international guidelines recommended to perform skin prick tests and basophil activation tests (BAT) in people experiencing allergic reaction to the first dose of COVID-19 vaccine or with a history of PEG allergy. The aim of this review is to discuss the main potential mechanisms of immediate allergy to COVID19 vaccines based on published data, together with the various techniques used to confirm or not sensitization to one component.

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Flow cytometry analysis of anti-polyethylene glycol antibodies in human plasma

Cited by 25 publications

References 30 publications

Nanoparticle Surface Engineering with Heparosan Polysaccharide Reduces Serum Protein Adsorption and Enhances Cellular Uptake

Nanoparticle Surface Engineering with Heparosan Polysaccharide Reduces Serum Protein Adsorption and Enhances Cellular Uptake

The role and impact of polyethylene glycol on anaphylactic reactions to COVID-19 nano-vaccines

Immediate hypersensitivity to COVID-19 vaccines: Focus on biological diagnosis

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