Flow cytometry analyses of phagocytic and respiratory burst activities and cytochemical characterization of leucocytes isolated from wrasse (Labrus bergylta A.)

Haugland, Gyri Teien; Rønneseth, Anita; Wergeland, Heidrun I.

doi:10.1016/j.fsi.2014.04.023

Cited by 27 publications

(6 citation statements)

References 44 publications

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“…The nature of these cells is currently not known, but similar cells have been reported from trout (Li et al, 2006), Atlantic salmon (Haugland et al, 2012b) and wrasse (Haugland et al, 2014). We have earlier suggested for salmon and wrasse that these cells may be precursor cells or immature cells (Haugland et al, 2012a(Haugland et al, , 2014. They stain strongly positive for AcP and they may possibly belong to the myeloid linage, be a DC or B cell precursor.…”

Section: Discussionsupporting

confidence: 60%

“…These small mononuclear cells, which had ingested numerous beads, were easily observed in flow cytometry analysis and colorrapid staining of phagocytic cells from PBL. The nature of these cells is currently not known, but similar cells have been reported from trout (Li et al, 2006), Atlantic salmon (Haugland et al, 2012b) and wrasse (Haugland et al, 2014). We have earlier suggested for salmon and wrasse that these cells may be precursor cells or immature cells (Haugland et al, 2012a(Haugland et al, , 2014.…”

Section: Discussionsupporting

confidence: 56%

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Functional characterization of IgM+ B cells and adaptive immunity in lumpfish (Cyclopterus lumpus L.)

Rønneseth

Ghebretnsae

Wergeland

et al. 2015

Developmental & Comparative Immunology

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Section: Discussionsupporting

confidence: 60%

Section: Discussionsupporting

confidence: 56%

Functional characterization of IgM+ B cells and adaptive immunity in lumpfish (Cyclopterus lumpus L.)

Rønneseth

Ghebretnsae

Wergeland

et al. 2015

Developmental & Comparative Immunology

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“…The observation that wrasse with no gross gill lesions may be PCR-positive could be due to differences in host response for the 2 fish species. For instance, ballan wrasse have a large number of characteristic eosinophilic cells (Reite 2005, Haugland et al 2014. In addition, large numbers of rodlet cells, which are suggested to be important for fighting parasitic infestations, have been found in Labrids (Reite 2005, Reite & Evensen 2006.…”

Section: Discussionmentioning

confidence: 99%

Atlantic salmon Salmo salar and ballan wrasse Labrus bergylta display different susceptibility to clonal strains of Paramoeba perurans

et al. 2020

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Amoebic gill disease (AGD), caused by the marine amoeba Paramoeba perurans, is an important disease of farmed Atlantic salmon Salmo salar L. in Norway. The use of wrasse as cleaner fish in salmon net pens raises questions about interspecies transmission of pathogens such as P. perurans. In this study, cohabitant transmission of clonal isolates of P. perurans between Atlantic salmon and ballan wrasse Labrus bergylta Ascanius was examined, using isolates originating from both salmon and wrasse. The challenges resulted in AGD in both species, although less severely in wrasse. The amoeba isolate originating from ballan wrasse was more virulent than that originating from salmon, suggesting P. perurans strain-related virulence differences. The isolate originating from salmon showed limited proliferation in bath-challenged wrasse and salmon, and limited transfer to cohabitants. Our results support previous observations suggesting that salmon may be more susceptible to P. perurans and AGD than ballan wrasse. Treatment of P. perurans infection in wrasse is challenging, as it is a strictly marine fish species. In this study, brackish water (<15‰ seawater) treatment of AGD affected salmon and wrasse was examined. Both salmon and wrasse were treated for short periods (3 h and 24 h), and treatment of wrasse over longer periods (3-5 d) was also examined. Short exposure to brackish water was not enough to remove P. perurans, although the 24 h treatment reduced amoeba levels. It was not possible to culture or detect P. perurans from wrasse exposed to brackish water for 3 d, suggesting that this treatment would be effective in controlling the parasite.

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“…Manual counts are costeffective but labor-intensive. Flow cytometry costs more but provides higher throughput and allows for simultaneous measurements of phagocytosis and respiratory burst (160,161). An advantage of both types of cell counts is that they are applicable to many species.…”

Section: Tools and Areas Of Consideration For Ecoimmunological Studiesmentioning

confidence: 99%

The Costs of Living Together: Immune Responses to the Microbiota and Chronic Gut Inflammation

Kirschman

Milligan-Myhre

2019

Appl Environ Microbiol

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While the vertebrate microbiota is critical to the normal function of many host traits, hosts may expend a large amount of energy to constrain and interface with their microbiota via their immune system to avoid the high fitness costs associated with gut dysbiosis, pathobionts, and opportunistic pathogens. All jawed vertebrates share mucosal immunity dedicated to isolating the microbiota, and a breakdown of this system can result in chronic gut inflammation. In humans, chronic gut inflammation negatively affects growth and development. There is little information available on the prevalence of chronic gut inflammation in wild animals, but given that animals with different life histories emphasize different immune responses, it follows that wild animals may vary in their susceptibility to chronic gut inflammation, and most animals will experience signaling that can lead to this state. These can be top-down signals originating from sources like the central nervous system or bottom-up signals originating from changes in the gut microbiota. The sources of these signals might include stress, developmental transitions, food restriction, and dietary shifts. Here, we briefly discuss host-microbiota interactions from the perspective of life history theory and ecoimmunology, focusing on the mucosal immune system and chronic gut inflammation. We also include future directions for research and the tools necessary to investigate them.

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Flow cytometry analyses of phagocytic and respiratory burst activities and cytochemical characterization of leucocytes isolated from wrasse (Labrus bergylta A.)

Cited by 27 publications

References 44 publications

Functional characterization of IgM+ B cells and adaptive immunity in lumpfish (Cyclopterus lumpus L.)

Functional characterization of IgM+ B cells and adaptive immunity in lumpfish (Cyclopterus lumpus L.)

Atlantic salmon Salmo salar and ballan wrasse Labrus bergylta display different susceptibility to clonal strains of Paramoeba perurans

The Costs of Living Together: Immune Responses to the Microbiota and Chronic Gut Inflammation

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