Flow Cytometry: An Introduction

Abstract

A flow cytometer is an instrument that illuminates cells (or other particles) as they flow individually in front of a light source and then detects and correlates the signals from those cells that result from the illumination. In this chapter, each of the aspects of that definition will be described: the characteristics of cells suitable for flow cytometry, methods to illuminate cells, the use of fluidics to guide the cells individually past the illuminating beam, the types of signals emitted by the cells and the detection of those signals, the conversion of light signals to digital data, and the use of computers to correlate and analyze the data after they are stored in a data file. The final section of the chapter will discuss the use of a flow cytometer to sort cells. This chapter can be read as a brief, self-contained survey. It can also be read as a gateway with signposts into the field. Other chapters in this book will provide more details, more references, and even an intriguing view of the future of cytometry.

Flow cytometry in cancer stem cell analysis and separation

“…By passing a laser beam, the type of event is identified and the droplets containing the events of interest become charged electrostatically. The droplets are then separated by their electrostatic charges in a high voltage field and collected in separate tubes, 96-well plates or slides (144,146,147). However, the jet-in-air cell presentation provides less optical sensitivity than cuvette systems and may negatively influence the identification of extremely rare target cells (119).…”

Rapid Detection of Extended-Spectrum Beta-Lactamases by Flow Cytometry Method

“…Akım sitometrisi, özellikle standart duyarlılık test yöntemleri ile sonuç alınmasının uzun sürdüğü mikobakteri ve mantar gibi mikroorganizmaların antibiyotik duyarlılık sonuçlarının kısa sürede alınmasında da kullanılmakta-dır 23 . Bu yöntem ayrıca, antimikrobiyal ajanların bakteriyostatik ve bakterisidal etkilerini göstermekte de yararlıdır ve minimal bakterisidal konsantrasyon değerlerini de tespit edebilmektedir 6 . Faria-Ramos ve arkadaşları 10 , 41 GSBL pozitif ve 20 GSBL negatif olmak üzere toplam 61 örnekte akım sitometri yöntemini değerlendirmişler ve yöntemin GSBL tespitinde hızlı ve doğru sonuçlar verdiğini bildirmişlerdir.…”

“…Dünya Savaşı döneminde aerosoller içerisindeki bakterileri tespit etmek amacıyla kullanılmıştır. 1970'lerin ortasından itibaren mikrobiyoloji alanında akım sitometrisinin kullanımı başlamıştır 6 . Bu çalışmanın amacı, GSBL üreten bakterilerin daha kısa sürede tespit edilmesini sağlayan akım sitometrisi temelli test yönteminin değerlen-dirilmesidir.…”

Circulating endothelial cells and serum visfatin are indicators of cardiovascular disease risk in psoriasis patients

“…CECs were evaluated using gate for mononuclear cells and data were expressed as cells/mL of blood ( Figure 1). 16,17 The mean, median, maximal, minimal, and standard deviation of values was calculated. The significance of differences between categorical variables were determined by Pearson's Chi-square test or Chisquare with Fisher's exact test.…”