Flow cytometric methodology for the detection of de novo human T-cell leukemia virus -1 infection in vitro: A tool to study novel infection inhibitors

Abstract

HighlightsMT-2 cells express more Tax, gp46 and p19 than HUT102′s.HUT78 cells express higher levels of the HTLV-1 permissive receptors neuropilin and GLUT-1 than CEM or JURKAT.Irradiation does not eliminate all MT-2 donor cells in HTLV-1 co-culture protocols.Flow cytometry and Lt-4 anti-tax antibody can detect de novo HTLV-1 infection at early time points.Cytochalasin B and sodium valproate inhibit HTLV-1 infection at early time points.

Acoustofluidic cell micro-dispenser for single cell trajectory control

“…1,2 Knowing the number of input cells is important to reliably monitor cell health, proliferation, transfection, and for standardising experiments. 2 Current available technologies including fluorescence-activated cell sorting (FACS), 3,4 flow cytometry, 5,6 and Coulter counters 7,8 are high-throughput, flow through systems, meaning that cells are counted as they pass through a measurement point. Although these platforms provide accurate counting and transferring of large cell volumes, their capabilities diminish when low cell concentrations are involved.…”