Flow cytometric evaluation of sperm apoptosis in semen of silver foxes in the breeding period

Kostro, K.; Krakowski, Leszek; Lisiecka, Urszula; Jakubczak, Andrzej; Żmuda, Andrzej; Wojtaszczyk, P.; Wąchocka, A.

doi:10.1016/j.anireprosci.2013.10.006

Cited by 5 publications

(3 citation statements)

References 9 publications

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“…The next events observed during the intrinsic apoptotic cascade are the activation of caspases in the sperm cytosol and the surface expression of phosphatidylserine, as detected by annexin V binding. Both of these features have been repeatedly observed in spermatozoa undergoing apoptosis within several species including man, 38 rabbit, 39 buffalo, 40 bull, 41 dog, 42 boar, 43 silver fox, 44 stallion 45 and ram. 46 In light of observations in other cell types in seems reasonable to propose that the phagocytosis of moribund spermatozoa is triggered by the surface expression of phosphatidylserine in weakly motile, apoptotic spermatozoa.…”

Section: Life and Death In The Germ Linementioning

confidence: 74%

Are sperm capacitation and apoptosis the opposite ends of a continuum driven by oxidative stress?

2015

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This chapter explores the possibility that capacitation and apoptosis are linked processes joined by their common dependence on the continued generation of reactive oxygen species (ROS). According to this model capacitation is initiated in spematozoa following their release into the female reproductive tract as a consequence of intracellular ROS generation, which stimulates intracellular cAMP generation, inhibits tyrosine phosphatase activity and enhances the formation of oxysterols prior to their removal from the sperm surface by albumin. The continued generation of ROS by capacitating populations of spermatozoa eventually overwhelms the limited capacity of these cells to protect themselves from oxidative stress. As a result the over-capacitation of spermatozoa leads to a state of senescence and the activation of a truncated intrinsic apoptotic cascade characterized by enhanced mitochondrial ROS generation, lipid peroxidation, motility loss, caspase activation and phosphatidylserine externalization. The latter may be particularly important in instructing phagocytic leukocytes that the removal of senescent, moribund spermatozoa should be a silent process unaccompanied by the generation of proinflammatory cytokines. These observations reveal the central role played by redox chemistry in defining the life and death of spermatozoa. A knowledge of these mechanisms may help us to engineer novel solutions to both support and preserve the functionality of these highly specialized cells.

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Section: Life and Death In The Germ Linementioning

confidence: 74%

Are sperm capacitation and apoptosis the opposite ends of a continuum driven by oxidative stress?

2015

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“…One of the earliest events observed during the intrinsic apoptotic cascade in spermatozoa is a sudden increase in the generation of mitochondrial ROS and a concomitant loss of sperm motility (Koppers et al., ). The next events observed during the intrinsic apoptotic cascade are the activation of caspases in the sperm cytosol and the expression of phosphatidylserine in the external layer of the plasmalemma (Kostro et al., ; Mohan & Atreja, ; Zeng et al., ; Zhao et al., ). Our results showed that OSS caused an increase in the proportion of spermatozoa showing events that could be considered as harbingers of apoptosis (YO‐PRO‐1 + ).…”

Section: Discussionmentioning

confidence: 99%

Oestrous sheep serum balances ROS levels to supply in vitro capacitation of ram spermatozoa

Olmo

García-Álvarez

Maroto-Morales

et al. 2016

Reprod Domestic Animals

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Reactive oxygen species (ROS) are fundamental for intracellular signalling. In spermatozoa, they are involved both to apoptosis and to capacitation, and changes in ROS levels can alter the balance between these two processes. Oestrous sheep serum (OSS) is considered an efficient agent for in vitro capacitation of ram spermatozoa. We have explored the effects of OSS on ram sperm physiology, especially on ROS production, during in vitro capacitation. Semen samples from 15 rams were cryopreserved. After thawing, samples were submitted to four treatments: control (CTL), 10% OSS supplementation for in vitro sperm capacitation, caspase inhibitor (INH, Z-VAD-FMK 100 μM) and OSS (10%) plus caspase inhibitor (I + E). Sperm samples were incubated for 30 min at 38.5°C and 5% CO2 and evaluated motility and kinetic parameters by computer-assisted semen analysis (CASA) and viability (propidium iodide), apoptotic-like membrane changes (YO-PRO-1), acrosomal status (PNA-FITC), intracellular calcium (FLUO-3), membrane fluidity (M540) and ROS production (CM-H2 DCFDA) by flow cytometry. OSS induced changes in kinetic parameters compatible with capacitation, with a decrease in the percentage of progressive motility and linearity, and an increase in the amplitude of the lateral displacement of the sperm head (p < .05). Moreover, OSS increased the proportion of M540+ viable spermatozoa, YO-PRO-1+ and acrosome-reacted spermatozoa (p < .05). After incubation, OSS and I+E achieved lower ROS levels (p < .05). Ca(2+) levels did not change with the incubation, but were slightly higher (p < .05) when both OSS and the inhibitor were present. We suggest that OSS may modulate ROS levels, allowing intracellular signalling for capacitation to occur while preventing higher levels that could trigger apoptosis.

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“…Unstained samples were used as the control groups, samples stained with only Annexin V-FITC were used Annexin V-FITC controls, and samples stained with only PI were used PI controls. Sperms were divided into four groups (Kostro et al, 2014), namely viable sperm (Annexin V-FITC − /PI − ), early apoptotic sperm (Annexin V-FITC + /PI − ), early necrotic apoptotic sperm (Annexin V-FITC + /PI + ) and necrotic sperm (Annexin V-FITC − /PI + ).…”

Section: Phosphatidylserine (Ps) Translocation Assaymentioning

confidence: 99%

Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex‐sorted bull sperm by inhibiting the oxidative stress

Zhang

Wang

et al. 2020

Reprod Domestic Animals

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The fertilization capacity of sex‐sorted sperms is seriously decreased, which inhibits its wide application. However, little information is still available about the effect of vitamin C (VC) and lycopene (Lyc) on the fertilization capacity of sex‐sorted bull sperm. In this study, the washing medium and fertilization medium of sex‐sorted sperm from three bull individuals were supplemented with different concentrations of VC (0, 1 × 10–3, 1 × 10–4, 1 × 10–5, 1 × 10–6 M) or Lyc (0, 1 × 10–4, 1 × 10–5, 1 × 10–6, 1 × 10–7). After washing twice and incubation for 1.5 hr, the malondialdehyde (MDA) level, phosphatidylserine (PS) translocation, membrane potential (Δψm) and IVF (in vitro fertilization) ability of sex‐sorted sperm were investigated. For the sex‐sorted sperm of bulls A, B and C, 1 × 10–3 M VC or 1 × 10–4 M Lyc treatment significantly decreased their MDA levels and PS translocation and increased their Δψm levels and cleavage rates after IVF. When blastocysts were concerned, 1 × 10–4 M Lyc significantly improved the blastocyst rates and their IFN‐tau expression of bulls A and C. In conclusion, supplementation of 1 × 10–3 M VC or 1 × 10–4 M Lyc in washing and fertilization medium contributed greatly to improving the fertilization capacity of sex‐sorted bull sperm during IVF procedure.

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Flow cytometric evaluation of sperm apoptosis in semen of silver foxes in the breeding period

Cited by 5 publications

References 9 publications

Are sperm capacitation and apoptosis the opposite ends of a continuum driven by oxidative stress?

Are sperm capacitation and apoptosis the opposite ends of a continuum driven by oxidative stress?

Oestrous sheep serum balances ROS levels to supply in vitro capacitation of ram spermatozoa

Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex‐sorted bull sperm by inhibiting the oxidative stress

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