Flow cytometric evaluation of sperm parameters in relation to fertility potential

Gillan, L.; Evans, G.; Maxwell, W.M.C.

doi:10.1016/j.theriogenology.2004.09.024

Cited by 229 publications

(173 citation statements)

References 64 publications

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“…The FC can advantageously be used instead of epifluorescent microscopy, 10 allowing determination of acrosome reaction and its temporal occurrence, and the examination of large sperm numbers. The most commonly used probes are plant lectins labeled with a fluorescent agent (usually FITC), most commonly PSA or peanut agglutinin (PNA).…”

Section: Sperm Intactnessmentioning

confidence: 99%

“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14] Most of these reviews and the literature cited therein consistently concur to a basic fact; routine spermiogram evaluations are suitable to identify clear-cut cases of infertility and, sometimes, to provide cues for potential subfertility, but are not able to prognose levels of fertility of the semen (or the male) in question. 7,13,15,16 The reason is quite obvious: such assessments simply evaluate basic variables of sperm appearance and viability, but cannot disclose subjacent structural details, biochemical and metabolic details, or the capacity of spermatozoa to interact with the environment, in terms of membrane reactivity, unless a battery of tests is used.…”

Section: Introductionmentioning

confidence: 99%

“…Markers that can determine how extensive the damages are, and that can monitor their occurrence can be used for a quick, detailed analyses of a large number of spermatozoa of a given sample. 10,18 Continuous innovation of new fluorescent stains and techniques has facilitated this development. Moreover, with the simplification of the instrumentation, flow cytometry (FC) is now being introduced into handling of semen for AI and even into routine andrological diagnostics.…”

Section: Introductionmentioning

confidence: 99%

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Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Hossain¹,

Johannisson²,

Wallgren³

et al. 2011

Asian J Androl

135

120

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Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The availability of 'bench-top' flow cytometers and of newer and versatile markers for cell structure and function had allowed the instrumentation to measure more sperm parameters, from viability to reactiveness when exposed to exogenous stimuli, and to increase our capabilities to sort spermatozoa for potential fertilizing capacity, or chromosomal sex. The present review summarizes the state of the art regarding flow cytometry applied to animal andrology, albeit keeping an open comparative intent. It critically evaluates the present and future capabilities of flow cytometry for the diagnostics of potential fertility and for the development of current reproductive technologies such as sperm freezing, sperm selection and sperm sorting. The flow cytometry methods will probably further revolutionize our understanding of the sperm physiology and their functionality, and will undoubtedly extend its application in isolating many uncharacterized features of spermatozoa. However, continuous follow-up of the methods is a necessity owing to technical developments and the complexity of mapping spermatozoa.

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Section: Sperm Intactnessmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Hossain¹,

Johannisson²,

Wallgren³

et al. 2011

Asian J Androl

135

120

View full text Add to dashboard Cite

show abstract

“…Lucifer Yellow CH is a membrane-impermeant compound (Peracchia, 1981), with a structure that enables it to be aldehyde-fixed into tissues with which it comes into contact (Heinrichs, 1985). Propidium iodide and Hoechst 33258 have been used as indicators of membrane integrity (Gillan et al 2005). For ease of expression, these molecules are referred to below as ' labelled compounds '.…”

Section: Fluorescent Chemicals Used In the Studymentioning

confidence: 99%

Schistosoma mansonicercariae experience influx of macromolecules during skin penetration

et al. 2009

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S U M M A R YWe have observed that when cercariae penetrate the skin of mice, there is influx into their tissues of Lucifer Yellow and certain labelled molecules of up to 20 kDa molecular weight. This observation was made using a variety of fluorescent membrane-impermeant compounds injected into the skin before the application of cercariae. This unexpected phenomenon was investigated further by transforming cercariae in vitro in the presence of the membrane-impermeant compounds and examining the distribution by microscopy. In schistosomula derived from this procedure, the nephridiopore and surface membrane were labelled while the pre-and post-acetabular glands were not labelled. The region associated with the oesophagus within the pharyngeal muscle clearly contained the fluorescent molecules, as did the region adjacent to the excretory tubules and the germinal mass. We used cercariae stained with carmine to aid identification of regions labelled with Lucifer Yellow. Although the mechanism of this influx is unclear, the observation is significant. From it, we can suggest an hypothesis that, during skin penetration, exposure of internal tissues of the parasite to external macromolecules represents a novel host-parasite interface.

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“…A third test, which is not targeted at detecting capacitational changes but is becoming increasingly common, is detection of changes in sperm mitochondrial transmembrane potential using such probes as MitoTracker TM (MITO), JC-1 (5,5 0 ,6,6 0 -tetrachloro-1,1 0 ,3,3 0 -tetraethylbenzimidazolyl-carbocyanine iodide), and Rhodamine 123 (91,92). The transmembrane potential is related to the mitochondrion's ability to produce ATP via oxidative phosphorylation.…”

Section: Capacitationmentioning

confidence: 99%

Cytometric solutions in veterinary andrology: Developments, advantages, and limitations

Petrunkina

Harrison²

2011

Cytometry Pt A

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Cytometric methodologies are becoming increasingly important in veterinary andrology as means of assessing sperm function. However, as yet, flow cytometric techniques in veterinary andrology have not kept up in sophistication with those in other areas of biology and medicine. In this brief review, we consider the present state of cytometry in andrological procedures for evaluating the fertility of domestic animal sires. We outline the aspects of sperm physiology, paying particular attention to the changes that take place during the process known as capacitation, which prepares the sperm for interaction with the egg. We then examine briefly but critically the cytometric techniques that are currently in commercial use or are being established in research laboratories for testing sperm characteristics. Current limitations and potential developments in semen assessment are discussed. Recent research knowledge offers possibilities for applying more subtle flow cytometric approaches to distinguish different levels of fertilizing potential in semen samples. For example, linking field fertility data to multiparametric kinetic studies of sperm capacitational changes rather than ''single parameter-single time point'' estimations may reveal that slower rather than rapid changes indicate high fertility. Moreover, the development of multicolor flow cytometric procedures as a means of evaluating multiple functional parameters in individual cells would reduce the uncertainties always inherent in predicting fertility from in vitro sperm evaluation tests. ' 2011 International Society for Advancement of Cytometry Key terms fertility; sperm evaluation; heterogeneity; subpopulations; multicolour cytometry THE main focus of an andrological assessment in veterinary medicine is to ascertain the fertility of a potential sire. Although the most obvious and indeed frequently used general veterinary test is to quantify the number of offspring born to the sire, this is a lengthy and very expensive process. For ''accuracy,'' many inseminations must be carried out, and results must await gestation and birth. Obviously, therefore, development of rapid tests that can be carried out in the laboratory has for long been important. However, as research into the reproductive process has advanced, it has become clear that the life of the fertilizing sperm between its release from the testis and its fusion with the egg is far from simple. Moreover, the population of sperm ejaculated into the female has been revealed as being heterogeneous in functional ability, even if morphologically normal [c.f. reviews (1,2)]. As a result of these findings, tests are being developed to examine functional ability within a heterogeneously responding population (3-5).Given that a normal male ejaculate contains many millions of individual sperm cells, it is natural that these tests are based on cytometry. In this short review, we will outline and comment upon a range of modern and still-developing cytometric methodologies in use or potentially useful for sperm...

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Flow cytometric evaluation of sperm parameters in relation to fertility potential

Cited by 229 publications

References 64 publications

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Schistosoma mansonicercariae experience influx of macromolecules during skin penetration

Cytometric solutions in veterinary andrology: Developments, advantages, and limitations

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