Flow cytometric DNA ploidy pattern in dysplastic mucosa, and in primary and metastatic carcinomas in patients with longstanding ulcerative colitis

Meling, Gunn Iren; Clausen, O. P. F.; Bergan, A.; Schjølberg, Aasa R.; Rognum, Torleiv O.

doi:10.1038/bjc.1991.302

Cited by 29 publications

(18 citation statements)

References 33 publications

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“…It was thus assumed that the preoper ative analysis of DNA ploidy pattern was useful in pre dicting the therapeutic schedule for patients with gastric cancer. 482 Kimura/Kanno/Takamura/Arakawa/ Maeda/Uogishi/Sodani/Kawashima Ancuploidy has also been associated with noncancerous diseases or lesions, such as ulcerative colitis [16] or colonic epithelium adjacent to colorectal cancer [12], Nine of our patients with gastric remnant had aneuploidy without microscopic confirmation of carcinoma. All the D1 belonged to the near-diploid area (0.8 £ DI ;£ 1.2, DI 4= 1.0).…”

Section: Discussionmentioning

confidence: 99%

Implications of Flow Cytometry in Preoperative Detection of Biologic Variables of Gastric Cancer and Malignant Condition of Gastric Remnant Cells Obtained by Endoscopic Biopsy

Kimura

Kanno²,

Takamura³

et al. 1994

Oncology

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Flow cytometric (FCM) DNA studies were performed on cell suspensions obtained by endoscopic biopsy in 73 patients with gastric cancer and in 61 patients with gastric remnant. In cancer, DNA aneuploidies were detected in 49 tumors (67.1 %), including 51.7% (15/29) of early gastric cancers and 77.3% (34/44) of advanced gastric cancers. Nuclear DNA content of biopsy materials had a significant correlation with that of surgically obtained ones, providing the estimation of the original ploidy pattern and DNA index (p < 0.01). The serosal invasion, lymph node metastasis, lymphatic invasion, and vascular invasion of the DNA aneuploidy were significantly higher than those of the DNA diploidy (p < 0.05). In patients with gastric remnant, 9 biopsy specimens in the anastomotic site showed DNA aneuploidy (13.9%). The DNA measurement of the endoscopically obtained specimens may provide additional preoperative information and help to identify biologic variables.

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Section: Discussionmentioning

confidence: 99%

Implications of Flow Cytometry in Preoperative Detection of Biologic Variables of Gastric Cancer and Malignant Condition of Gastric Remnant Cells Obtained by Endoscopic Biopsy

Kimura

Kanno²,

Takamura³

et al. 1994

Oncology

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“…The mean coefficient of variation (CV) of the diploid peak was 3.2 (range 1.1-5.5). A tumour was defined as aneuploid ( Figure 2b) if a second distinct population of G, cells was present, and had a DNA index> 1.10 (Kirkhus et al, 1988;Meling et al, 1991). Otherwise the tumour was defined as near diploid (Figure 2a).…”

Section: Definition Of Aneuploidymentioning

confidence: 99%

“…Demonstration of an aneuploid DNA pattern reflects gross chromosomal changes, and may indicate genetic instability in the tumour cells (Rognum et al, 1983;Meling et al, 1991). Furthermore, the presence of aneuploidy is an important prognostic variable in colorectal carcinomas (Wolley et al, 1982;Armitage et al, 1985;Rognum et al, 1987.…”

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confidence: 99%

Genetic alterations within the retinoblastoma locus in colorectal carcinomas. Relation to DNA ploidy pattern studied by flow cytometric analysis

Meling

Lothe²,

Børresen³

et al. 1991

Br J Cancer

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Alterations within the retinoblastoma (Rb) gene, as detected by the VNTR probe p68RS2.0, and flow cytometric DNA pattern have been analysed in 255 colorectal carcinomas. A total of 35.3% of the tumours had alterations within the Rb gene. Amplification of one allele was demonstrated in 29.5% of the tumours, and loss of heterozygosity was found in 11.5%. No association was found between amplification within the Rb gene and clinicopathological characteristics of the patients. The high frequency of alterations demonstrated within the Rb gene, suggests that this gene is involved in colorectal carcinogenesis with amplification as by far the most abundant genetic alteration. This may imply that the Rb gene has an oncogene-like function in colorectal carcinomas, rather than acting as a tumour suppressor gene. Sixty-three per cent of the carcinomas were DNA aneuploid, and a significant association was demonstrated between amplification within the Rb gene and DNA aneuploidy (P less than 0.01). Two other chromosome loci were analysed, on chromosome 1p (probe pYNZ2) and on chromosome 2p (probe pYNH24), respectively. On chromosome 1p, heterozygous loss was found in 22.2% of the tumours, indicating an involvement of this chromosome in a subset of colorectal carcinomas. Images Figure 1

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“…Tumor development in UC is associated with dysplastic changes that mostly precede invasive carcinoma and thus constitute an early marker of malignant transformation. It has been previously shown that DNA aneuploidy is present in nondysplastic and dysplastic and carcinomatous mucosa in UC (2,3), and that DNA aneuploidy in colonic biopsies may predict future development of dysplasia (4). More than one half of the tumors developing in UC harbor DNA aneuploid populations as measured by DNA flow cytometry (FCM; 2).…”

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confidence: 99%

A strategy combining flow sorting and comparative genomic hybridization for studying genetic aberrations at different stages of colorectal tumorigenesis in ulcerative colitis

Clausen¹,

Andersen²,

Strømkjær³

et al. 2000

Cytometry

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Flow cytometric DNA ploidy pattern in dysplastic mucosa, and in primary and metastatic carcinomas in patients with longstanding ulcerative colitis

Cited by 29 publications

References 33 publications

Implications of Flow Cytometry in Preoperative Detection of Biologic Variables of Gastric Cancer and Malignant Condition of Gastric Remnant Cells Obtained by Endoscopic Biopsy

Implications of Flow Cytometry in Preoperative Detection of Biologic Variables of Gastric Cancer and Malignant Condition of Gastric Remnant Cells Obtained by Endoscopic Biopsy

Genetic alterations within the retinoblastoma locus in colorectal carcinomas. Relation to DNA ploidy pattern studied by flow cytometric analysis

A strategy combining flow sorting and comparative genomic hybridization for studying genetic aberrations at different stages of colorectal tumorigenesis in ulcerative colitis

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