Flow cytometric DNA analysis of wheat‐rye addition lines

Bashir, Aftab; Auger, Julie; Rayburn, A. Lane

doi:10.1002/cyto.990140802

Cited by 22 publications

(12 citation statements)

References 19 publications

(10 reference statements)

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“…The gain or loss of a single chromosome can be detected by this method, as shown in Musa (Roux et al 2003), ryegrass (Barker et al 2001), and wheat-rye addition lines (Bashir et al 1993, Pfosser et al 1995. Moreover, Yamaguchi et al (2008) reported that nuclear DNA content of chrysanthemums decreased with increasing dose and dose rate of gamma rays, indicating that the nuclear DNA content could be used as an index of radiation damage.…”

Section: Introductionmentioning

confidence: 98%

Effects of ion beam irradiation on mutation induction and nuclear DNA content in chrysanthemum

et al. 2010

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We investigated the effects of ion beams, in comparison to gamma rays, on mutation induction and nuclear DNA content as an index of radiation damage in Chrysanthemum morifolium. Leaf segments were irradiated with 220 MeV carbon ions (mean linear energy transfer = 107 keV/µm), 320 MeV carbon ions (76 keV/µm), 100 MeV helium ions (9 keV/µm), and gamma rays. The nuclear DNA content was measured and frequency of flower color mutations was investigated in regenerated plants. The number of plants with reduced nuclear DNA content increased with increasing irradiation doses of 320 MeV carbon ions, 100 MeV helium ions, and gamma rays. In contrast, they did not increase with 220 MeV carbon ions, even when the dose was increased. Irradiation treatment with 220 and 320 MeV carbon ions and gamma rays had a similar effect on mutation induction, while the effect of 100 MeV helium ions was not as great. Thus, the effects of irradiation treatments on mutation induction and nuclear DNA content differed according to the type of ion beams. 220 MeV carbon ion beam seemed to be the most appropriate among the three types of ion beams because it gave a high mutation frequency with low damage to chromosomes.Key Words: chrysanthemum, flower color mutation spectrum, gamma rays, ion beams, mutation frequency, nuclear DNA content. IntroductionChrysanthemum is a widely cultivated ornamental plant worldwide. Mutation is an important tool in chrysanthemum breeding. Many mutant cultivars have been produced by spontaneous mutations (sports) and induced artificially by gamma or X-rays. In Japan, ion beams that consist of ion particles, accelerated by a cyclotron, have been used to attempt to induce mutations since the 1990s. Ion beams have high linear energy transfer (LET), and thus, greater biological effects compared to low LET radiation such as gamma and X-rays. Thus, ion beams are expected to produce higher mutation frequencies and novel mutants. It has been demonstrated that ion beams induce mutations at high frequency and produce broad mutation spectrum, including novel mutants, in Arabidopsis Hase et al. 2000, Tanaka et al. 1997. Recently, mutation induction by ion beam irradiation has been carried out in various plants, particularly ornamental plants such as carnation (Okamura et al. The use of ion beams for inducing mutations in chrysanthemum breeding has also been attempted (Furutani et al. 2008, Nagatomi et al. 1998, Shirao et al. 2007, Suzuki et al. 2005, Ueno et al. 2005, Wakita et al. 2008, Watanabe et al. 2008. Thus, ion beam-specific flower color mutants that could not be obtained using gamma rays were obtained by the abovementioned programs (Nagatomi et al. 1998 Although radiation treatment induces mutations, it causes radiation damage. Konzak et al. (1965) suggested that not only mutation induction effect but also the plant damage caused by irradiation treatment, such as chromosomal aberrations, should be considered for the use of any mutagen in plant breeding. Reduction in chromosome number was reported in chrysanthemums irrad...

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Section: Introductionmentioning

confidence: 98%

Effects of ion beam irradiation on mutation induction and nuclear DNA content in chrysanthemum

et al. 2010

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“…For example, flow cytometry has been used to determine plant ploidy levels and to detect aneuploidy (reviewed in Dolezel 1991;Bashir et al 1993;Pfosser et al 1995;Lee et al 1998). Flow cytometry can be used for the classification of chromosomes based on DNA content and the identification of chromosomal variation within species, or the detection of abnormal chromosomes (Gary et al 1988;Dolezel and Lucretti 1995;Lee et al 1996Lee et al , 2000Gill et al 1999;Lysak et al 1999;Vrana et al 2000).…”

Section: Introductionmentioning

confidence: 98%

Variability of chromosomal DNA contents in maize ( Zea mays L.) inbred and hybrid lines

Lee

Arumuganathan

Kaeppler

et al. 2002

Planta

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The flow karyotypes of different maize (Zea mays L.) inbred and hybrid lines were analyzed. The accumulation and isolation of large quantities of high-quality metaphase chromosomes from root tips was achieved from many kinds of maize lines. The chromosome suspensions were prepared by a simple slicing method from synchronized maize root tips and analyzed by flow cytometry. Variations of experimental flow karyotypes were detected among inbred and hybrid lines in terms of the positions and/or the numbers of chromosome peaks. The 2C DNA amount among eight inbred lines ranged from 5.09 to 5.52 pg. The selection of appropriate maize lines is critical for sorting specific single chromosome types. At least five different chromosome types can be discriminated and sorted from five maize lines. The variability of DNA content in maize chromosome 1 was 9.1%, ranging from 0.685 to 0.747 pg. Differences were detected in the DNA content of homologous chromosome 1 of hybrid lines.

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“…(Dolezel et al 1994. Bashir et al (1993) showed that flow cytometry is quite sensitive in detecting the presence of a pair of rye chromosomes in wheatrye addition lines. Pfosser et al (1995) demonstrated that this method might detect the presence of a pair of rye chromosome arms added to the wheat-rye addition lines.…”

Section: Introductionmentioning

confidence: 98%

Rapid detection of aneuploidy in Musa using flow cytometry

et al. 2002

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We report a procedure for the rapid and convenient detection of aneuploidy in triploid Musa using DNA flow cytometry. From a population of plants derived from gamma-irradiated shoot tips, plants were selected based on aberrant morphology and their chromosome numbers were counted. Aneuploids plants with chromosome numbers 2n=31 or 32 were found as well as the expected triploid plants (2n=3x=33). At the same time, the nuclear DNA content of all plants was measured using flow cytometry. The flow cytometric assay involved the use of nuclei isolated from chicken red blood cells (CRBC), which served as an internal reference standard. The relative DNA content of individual plants was expressed as a ratio of DNA content of CRBC and Musa (DNA index). In order to estimate the chromosome number using flow cytometry, the relative DNA content of plants with unknown ploidy was expressed as a percentage of the DNA content of triploid plants. The classification based on flow cytometry fully agreed with the results obtained by chromosome counting. The results indicated that flow cytometry is a convenient and rapid method for the detection of aneuploidy in Musa.

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Flow cytometric DNA analysis of wheat‐rye addition lines

Cited by 22 publications

References 19 publications

Effects of ion beam irradiation on mutation induction and nuclear DNA content in chrysanthemum

Effects of ion beam irradiation on mutation induction and nuclear DNA content in chrysanthemum

Variability of chromosomal DNA contents in maize ( Zea mays L.) inbred and hybrid lines

Rapid detection of aneuploidy in Musa using flow cytometry

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