Flow cytometric DNA analysis of ducks accumulating 137Cs on a reactor reservoir

George, LeighAnn; Dallas, Cham E.; Brisbin, I. Lehr; Evans, Donald L.

doi:10.1016/0147-6513(91)90073-x

Cited by 40 publications

(33 citation statements)

References 15 publications

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“…Two mallard ducks from the same site also showed DNA aneuploidy. After removal from exposure and later FCM analysis, DNA aneuploidy was seen only in one animal (George et al 1991).…”

Section: Discussionmentioning

confidence: 96%

“…As seen in work on T. scripta and A. platyrhynchos, repeated, non-lethal sampling provides valuable and possibly more ecologically relevant information than short-term studies George et al 1991;Lamb et al 1991Lamb et al , 1995. Lamb et al (1995) reported that CVs may undergo temporary fluctuations due to changes in population structure or metabolic responses to contaminants in the organisms of interest.…”

Section: Discussionmentioning

confidence: 97%

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Flow cytometric analysis of red-eared slider turtles (Trachemys scripta) from Tar Creek Superfund Site

Hays

McBee

2007

Ecotoxicology

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Tar Creek Superfund Site (TCSFS) was heavily mined from the 1890s to 1970 and currently is contaminated with lead, zinc, and cadmium. Flow cytometry (FCM) was used to measure variation in nuclear DNA content of red blood cells collected from Trachemys scripta living within TCSFS and reference sites, Lake Carl Blackwell (LCB) and Sequoyah National Wildlife Refuge (SNWR). We also used atomic absorption spectrometry to measure Pb in blood and carapace and Cd in blood samples of turtles from TCSFS and SNWR. Mean coefficients of variation around the G(1) peak ranged from 5.33 to 5.48 and showed no significant difference between contaminated and reference populations; however, there was a significantly higher frequency of aneuploidy at TCSFS when compared with both reference populations. Blood Pb levels were not significantly different between TCSFS and SNWR populations. Pb levels in carapace samples did not differ significantly between sites; however, Pb levels were higher in carapace than blood for both populations. Blood Cd was significantly higher in animals at TCSFS than SNWR.

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“…Two mallard ducks from the same site also showed DNA aneuploidy. After removal from exposure and later FCM analysis, DNA aneuploidy was seen only in one animal (George et al 1991).…”

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 97%

Flow cytometric analysis of red-eared slider turtles (Trachemys scripta) from Tar Creek Superfund Site

Hays

McBee

2007

Ecotoxicology

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“…FCM was used to estimate DNA content variation within red blood cells (RBC) of both European pond turtles (Emys orbicularis) and Caspian turtles (Mauremys caspica). This method has been shown to be an effective biomarker of chromosomal damage, caused by a variety of contaminants including PAHs, radionuclides, and some pesticides, in many wildlife studies McBee and Bickham, 1988;Dallas and Evans, 1990;George et al, 1991;Lamb et al, 1991Lamb et al, , 1995Bickham et al, 1992Bickham et al, , 1994Custer et al, 1994;Lingenfelser et al, 1997aLingenfelser et al, , 1997bLowcock et al, 1997;Theodorakis et al, 2001;Matson et al, in press). Of more importance to this study is the fact that flow cytometry data tend to correlate well with petroleum products and PAHs in particular (Bickham et al, 1998a;Custer et al, 2000).…”

Section: Introductionmentioning

confidence: 93%

Chromosomal Damage in Two Species of Aquatic Turtles (Emys orbicularis and Mauremys caspica) Inhabiting Contaminated Sites in Azerbaijan

et al. 2005

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The Caspian region and specifically the Apsheron peninsula of Azerbaijan are known to be polluted with a variety of environmental contaminants. These complex mixtures of contaminants make risk assessment difficult. We used the flow cytometry method (FCM) and the micronucleus assay (MN) to assess chromosomal damage in aquatic turtles (Emys orbicularis, the European pond turtle; and Mauremys caspica, the Caspian turtle) inhabiting contaminated wetlands in Azerbaijan. Evidence of genetic damage was found for two sites, Neftchala and Sumgayit, relative to a reference site, Ali Bairamly. Sediment samples from each site were analyzed for PAHs and mercury to evaluate potential contaminant associations with genetic damage. A significant positive correlation was documented between three-ring PAH sediment concentrations and FCM estimates of chromosomal damage in E. orbicularis. These data combine to show that the contaminated wetlands in Sumgayit and Neftchala are genotoxic and that three-ring PAHs are likely a significant influence on observed genotoxicity.

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“…However, SEM is unlikely to substitute for the rapid methods presently available to screen large numbers of fish for routine determination of ploidy. Additionally, some pollutants increase variation in DNA content of blood cells McBee and Bickham 1988;Lamb et al 1991 ;George et al 1991), and we speculate that changes in DNA content associated with genotoxicity may be directly observable by SEM as alterations in nuclear size, shape, or structure.…”

Section: Discussionmentioning

confidence: 96%

Quantitative comparisons of the morphology and ultrastructure of erythrocyte nuclei from seven freshwater fish species

Jagoe¹,

Welter²

1995

Can. J. Zool.

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Chromosome number and genomic DNA content vary widely among fish species, and ploidy can vary within species. This suggests that the size, shape, and morphological features of cell nuclei may also vary. Nucleated erythrocytes of fish are an easily sampled homogeneous population of differentiated cells ideal for inter-and intra-species comparisons. We collected blood samples from largemouth bass (Micropterus salmoides) , bluegill (Lepomis macrochirus) , chain pickerel (Esox niger) , yellow perch (Perca flavescens) , mosquitofish (Gambusia holbrooki) , redeye bass (Micropterus coosae), and rainbow trout (Oncorhynchus mykiss) and removed cytoplasm and nuclear membranes from blood cells. Individual nuclei were examined and measured using scanning electron microscopy and a computerized image analysis system, and inter-and intra-species differences evaluated by nested analysis of variance. Nuclear size and shape varied significantly among species. Isolated nuclei had conspicuous apertures or holes, and the number and size of these holes also varied significantly among species. Variations in nuclear size and structure within species were small compared with interspecies differences. Little is known of the ultrastructure of erythrocyte nuclei in lower vertebrates, but their structure differs considerably from that of other vertebrate non-erythroid cells, suggesting that the organization of their DNA and associated proteins may be different. RCsumC: Le nombre chromosomique et le contenu en ADN du gCnome varient considCrablement d'une espkce de poisson A une autre et la ploi'die peut varier au sein d'une espkce. Par consCquent, la taille, la forme et les caractkristiques morphologiques des noyaux cellulaires risquent de varier aussi. Les Crythrocytes nuclCCs de poissons constituent une population homogkne, facile A Cchantillonner, de cellules diffCrenciCes propices aux comparaisons inter-et intra-spCcifiques. Nous avons recueilli des Cchantillons de sang chez des Achigans A grande bouche (Micropterus salmoides), des Crapets arlequins (Lepomis macrochirus), des Brochets maillCs (Esox niger), des Perchaudes (Perca flavescens), des Truites arc-en-ciel (Oncorhynchus mykiss), des gambusies Gambusia holbrooki, et des achigans Micropterus coosae et avons prClevC le cytoplasme et les membranes nuclCaires dans les cellules sanguines. Les noyaux ont Ct C examinks et mesurCs chacun au moyen d'un microscope Clectronique A balayage et d'un systkme informatis6 d'analyse d'images; les differences inter-et intra-specifiques ont Ct C CvaluCes par une analyse de variance hikrarchique. La taille et la forme du noyau varient significativement d'une espkce A l'autre. Les noyaux isolCs comportent des orifices ou cavitCs bien visibles dont le nombre et la taille varient aussi de f a~o n significative selon l'espkce. Les variations de la taille et de la structure du noyau entre individus d'une meme espkce sont beaucoup moins importantes qu'entre des espkces diffkrentes. L'ultrastructure des noyaux des Crythrocytes est peu connue chez les vertCbrC...

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Flow cytometric DNA analysis of ducks accumulating 137Cs on a reactor reservoir

Cited by 40 publications

References 15 publications

Flow cytometric analysis of red-eared slider turtles (Trachemys scripta) from Tar Creek Superfund Site

Flow cytometric analysis of red-eared slider turtles (Trachemys scripta) from Tar Creek Superfund Site

Chromosomal Damage in Two Species of Aquatic Turtles (Emys orbicularis and Mauremys caspica) Inhabiting Contaminated Sites in Azerbaijan

Quantitative comparisons of the morphology and ultrastructure of erythrocyte nuclei from seven freshwater fish species

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