Flow cytometric detection of tumor metastases during urologic surgery

Nusbaum, Neil J.; Kester, Robert R.; Mooppan, Unni M.M.; Kim, Hong

doi:10.1016/0090-4295(91)80213-q

Cited by 4 publications

(5 citation statements)

References 11 publications

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“…Nussbaum et al [15] were not able to detect any cytokeratin-positive cells in the postoperative blood samples of any of their 11 patients, which included four with advanced prostatic carcinomas. In our group of patients, 14 of 15 with prostatic carcinoma had cytokeratin-positive cells in their postoperative blood samples.…”

Section: Discussionmentioning

confidence: 98%

“…On account of the low detection rate of cytokeratinpositive cells isolation of these cells by cell sorting was not possible. Nussbaum et al [15] were able to isolate cytokeratin-positive cells by cell sorting in two of four cases of prostatic carcinoma. The tissue of origin of the tumors is not mentioned by the authors.…”

Section: Discussionmentioning

confidence: 98%

“…In our group of patients, 14 of 15 with prostatic carcinoma had cytokeratin-positive cells in their postoperative blood samples. Nussbaum et al [15] used the lysis method for separation of the circulating tumor cells. In our preliminary experiments with mesothelial cells we were able to detect a higher rate of cytokeratin-positive cells by the Ficoll method than by the lysis method.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years increasing attention has been directed towards the use of monoclonal antibodies against cytokeratins to detect micrometastatic carcinoma cells [8,15]. Cytokeratins belong to the group of intermediate filaments, which are one of the main components making up the cytoskeleton of epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

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Detection of circulating prostatic cells during radical prostatectomy

Planz¹,

Szyska²,

Valdor³

et al. 1997

Urol. Res.

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The detection of micrometastasis of prostate cancer could help to decide more appropriate therapeutic strategies in an individual patient. We have developed a flow cytometric method for detecting cytokeratin-positive cells in the peripheral blood before, during and after radical prostatectomy in patients with prostatic carcinoma. By means of this technique we were able to detect a higher number of cytokeratin-positive cells in the intraoperative blood sample than in the pre- and postoperative blood sample in 15 patients with prostate cancer (P < 0.05). Our results show an increase in the number of cytokeratin-positive cells with increasing tumor stage and grade, as well a good correlation of prostate-specific antigen (PSA) value with the number of cytokeratin-positive cells (r > 0.6). Our results underline the importance of no-touch techniques at prostatectomy to minimize release of tumor cells into the circulation during surgery. In the light of our results we consider that the indication for cell savers during radical prostatectomy should be reevaluated. The possibility of detecting single metastatic cells in peripheral blood will enable better individual patient management, and open up new modalities for diagnosing early prostate cancer and enhancing patient monitoring in relapse and tumor progression.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of circulating prostatic cells during radical prostatectomy

Planz¹,

Szyska²,

Valdor³

et al. 1997

Urol. Res.

View full text Add to dashboard Cite

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“…The injection of tumor cells may still increase the intrarenal pressure, forcing tumor cells into the systemic and/or lymphatic circulation. Flow cytometric studies of peripheral blood in humans during urologic surgery have demonstrated an increased number of circulating nonhematologic cells during surgical manipulation [43]. In rabbits, surgical manipulation of VX-2 carcinoma implanted in the liver increased the number of lung metastases and reduced survival, compared to a not manipulated group of rabbits [44].…”

Section: Discussionmentioning

confidence: 99%

Comparison of nephrectomy and/or doxorubicin treatment in rabbit renal VX‐2 carcinoma

Gadeholt-Göthlin¹,

Göthlin²

1995

Journal of Surgical Oncology

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To compare survival after local intra-arterial and intravenous administration of doxorubicin, VX-2 carcinoma was implanted in one kidney of 48 rabbits. Treatments were given 9-14 days after tumor implantation. Survival after doxorubicin was significantly longer than the controls, whereas no difference was established between intra-arterial or intravenous treatment. Overall only 9 of 32 doxorubicin-treated rabbits were cured at autopsy after 18 months. It was assumed that circulating tumor cells from tumor implantation resulted in "primary" lung metastases. To kill circulating tumor cells intravenous doxorubicin was given immediately before implantation in 40 rabbits, followed by combinations of nephrectomy and doxorubicin. Doxorubicin without nephrectomy cured 8 of 16 rabbits, whereas doxorubicin combined with nephrectomy cured 4 of 16. Doxorubicin improved survival in responders, but likelihood of response was limited. Nephrectomy did not improve survival, most likely due to metastatic seeding at implantation. Intravenous doxorubicin immediately before did not prevent metastatic spread in connection with tumor implantation.

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Inhibition of pulmonary tumor seeding by antiplatelet and fibrinolytic therapy in an animal experimental model

Brown¹,

Purushotham²,

George³

1994

Journal of Surgical Oncology

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Fibrin and platelets contribute to the development of blood borne metastases by facilitating the arrest of tumor cell emboli in the microcirculation. We have previously demonstrated that the fibrinolytic agent streptokinase inhibits pulmonary tumor cell seeding in an animal model. To determine whether this effect was potentiated by antiplatelet therapy, a further study was performed to assess the effect of streptokinase in combination with aspirin in a similar model. The results demonstrated that aspirin did not significantly enhance the antimetastatic effect of streptokinase (median: streptokinase = 60, streptokinase + aspirin = 60.5, P = 0.79, Mann Whitney). In a second series of experiments, the antimetastatic effect of streptokinase was compared with another fibrinolytic agent, human recombinant tissue plasminogen activator (rt-PA). Fibrinolytic doses of streptokinase (30,000 units/kg) and rt-PA (5 mg/kg) significantly reduced pulmonary tumor seeding when compared with controls (median: control = 48, streptokinase = 23, P < 0.01, rt-PA = 29, P < 0.001). There was no significant difference in pulmonary tumor seeding between the groups treated with streptokinase and rt-PA (P = 0.56). The clinical implications of these findings are discussed.

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Flow cytometric detection of tumor metastases during urologic surgery

Cited by 4 publications

References 11 publications

Detection of circulating prostatic cells during radical prostatectomy

Detection of circulating prostatic cells during radical prostatectomy

Comparison of nephrectomy and/or doxorubicin treatment in rabbit renal VX‐2 carcinoma

Inhibition of pulmonary tumor seeding by antiplatelet and fibrinolytic therapy in an animal experimental model

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