Flow-Cytometric Deoxyribonucleic Acid Analysis of the Human Bladder Cancers with Reference to Histopathological Findings

Hamano, Katsuhiko; Kinjo, Mitsuru; Kumazawa, Joichi

doi:10.1159/000474744

Cited by 4 publications

(1 citation statement)

References 10 publications

(18 reference statements)

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“…Taken together, the data suggest that multiple biopsies can be important in some diploid tumours, since if only one sample is measured, there may be an undetected population exhibiting DNA aneuploidy. Hamano et al [29] reported that approximately 10% of histologically normal mucosa taken from patients with bladder cancer showed DNA aneuploidy suggesting that DNA-FCM of random biopsies of cystoscopically normal bladder mucosa of patients without tumours, may provide valuable data in predicting precancerous lesions.…”

Section: Discussionmentioning

confidence: 99%

DNA-Flow Cytometric Analysis of Bladder TCC Using Paraffin-Embedded Tissues

Wu¹,

Lochhead

Yang³

et al. 1998

Urol Int

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A retrospective study of DNA flow cytometry (FCM) in paraffin-embedded tissues of urinary bladder transitional cell carcinoma (TCC) was performed on 239 biopsy samples taken from 81 patients in the period from 1984 to 1994. 210 (87%) were analysable. Of these samples 21 patients had multiple biopsies taken from large tumours and/or bladder mucosa showing an endoscopically normal appearance. DNA-FCM results have been evaluated comparing ploidy and histopathological grade, clinical stage and different clinical status, i.e., first diagnosis, recurrence and patients who died from bladder cancer. Our results indicate that ‘diploid’ FCM correlated with a better prognosis, whilst DNA aneuploid correlated with malignancy and a poorer prognosis. There was a trend to an increasing incidence of DNA aneuploidy as the grade of the tumour rose and the proportion of biopsies with aneuploidy was significantly higher in malignant tissue samples, recurrences and in biopsies from patients who died from TCC than in other groups. In 12 patients from whom several biopsies were obtained, samples from recurrences had significantly higher DNA aneuploidy than those from the first diagnosis.

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Section: Discussionmentioning

confidence: 99%