Flow Cytometric Characterization of Murine B Cell Development

Harris, Faith M.; Meagher, Karoline A; Zhong, Maggie; Daniel, Benjamin J.; Eckersdorff, Mark; Green, Jesse  A.; Voronina, Vera A.; Guo, Chunguang; Limnander, André; Macdonald, Lynn E.

doi:10.3791/61565-v

Cited by 2 publications

(1 citation statement)

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“…To begin surveying the developmental B cell populations to determine if there was a defect, we used the following flow cytometric gating strategies for various stages of B cell development on cells extracted from femurs of pups (Fig. 2B) (21)(22)(23). There was no change in whole bone marrow cellularity (Fig.…”

Section: B Cell Development During Maternal S Mansoni Infection Is Im...mentioning

confidence: 99%

Maternal infection causes dysfunctional BCR signaling in male offspring due to aberrant Xist expression

Gibbs

Oviedo

Ondigo

et al. 2023

Preprint

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Infections during pregnancy with pathogens such as helminths correlate with altered immune responses to common childhood immunizations. However, the molecular mechanisms that underlie this remain unknown. Using our murine model of maternal schistosomiasis, when immunized, males from infected mothers had a lower frequency of antigen-specific germinal center B cells and downregulation of transcripts downstream of BCR signaling compared to males from uninfected mothers. This is driven by a reduction in developing B cell populations within the bone marrow of pups from infected mothers. Males from infected mothers were impacted to a greater extent than their female littermate counterparts. We found this defect to be caused by aberrant expression of the long non-coding RNA Xist in males leading to dysregulated Igalpha expression on developing B cells. This, for the first time, links dysfunctional BCR signaling with Xist expression, while also proposing a detrimental function for Xist expression in males.

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Section: B Cell Development During Maternal S Mansoni Infection Is Im...mentioning

confidence: 99%