Flow cytometric assessment of the antimicrobial activity of essential oils against Listeria monocytogenes

Paparella, Antonello; Taccogna, Lorenzo; Aguzzi, Irene; Chaves‐López, Clemencia; Serio, Annalisa; Marsilio, Fulvio; Suzzi, Giovanna

doi:10.1016/j.foodcont.2008.01.002

Cited by 129 publications

(85 citation statements)

References 50 publications

(54 reference statements)

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“…Oregano plant parts and biochemical extracts (herb, leaf, essential oil, etc.) are commonly used as spices in the food industry and are well known for their pharmacological properties, including as antimicrobials (12,22) and antioxidants (11,18). The antimicrobial activity of oregano essential oils, known since antiquity, is probably due to its high content of phenolic derivatives, such as carvacrol and thymol (5).…”

mentioning

confidence: 99%

Origanum vulgare subsp. hirtum Essential Oil Prevented Biofilm Formation and Showed Antibacterial Activity against Planktonic and Sessile Bacterial Cells

Schillaci

Napoli

Cusimano

et al. 2013

Journal of Food Protection

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Essential oils from six different populations of Origanum vulgare subsp. hirtum were compared for their antibiofilm properties. The six essential oils (A to F) were characterized by a combination of gas chromatography with flame ionization detector and gas chromatography with mass spectrometer detector analyses. All oils showed weak activity against the planktonic form of a group of Staphylococcus aureus strains and against a Pseudomonas aeruginosa ATCC 15442 reference strain. The ability to inhibit biofilm formation was investigated at sub-MIC levels of 200, 100, and 50 mg/ml by staining sessile cells with safranin. Sample E showed the highest average effectiveness against all tested strains at 50 mg/ml and had inhibition percentages ranging from 30 to 52%. In the screening that used preformed biofilm from the reference strain P. aeruginosa, essential oils A through E were inactive at 200 mg/ml; F was active with a percentage of inhibition equal to 53.2%. Oregano essential oil can inhibit the formation of biofilms of various food pathogens and food spoilage organisms.

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mentioning

confidence: 99%

Origanum vulgare subsp. hirtum Essential Oil Prevented Biofilm Formation and Showed Antibacterial Activity against Planktonic and Sessile Bacterial Cells

Schillaci

Napoli

Cusimano

et al. 2013

Journal of Food Protection

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“…The presence of free hydroxyl group in carvacrol and thymol caused depolarization of membrane and loss of potential. Differences in mode of action between esential oils (thyme, oregano and cinnamon) were demonstrated against listeria monocytogenes [22]. Thyme and oregano, rich in thymol and carvacrol, caused death of bacteria cells by membrane damage whereas cinnamon oil acted without any severe damage of membrane which suggests a different mode of action.…”

Section: Composition and Mechanism Of Antimicrobial Action Of Essentimentioning

confidence: 96%

Application of essential oils as natural cosmetic preservatives

Dreger

Wielgus

2013

Herba Polonica

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S u m m a r yNowadays, safety of chemical preservatives has been questioned by a big number of consumers. Traditionally used preservatives often cause skin irritation and lead to allergenic reactions. Growing demands for more natural and preservative-free cosmetics promoted an idea of the replacement of synthetic preservatives with essential oils (EOs) of antimicrobial properties. The antimicrobial effect of essential oil depends on content, concentration and interactions between the main active compounds. Effective preservatives should be characterized by a broad spectrum of antimicrobial activity at a minimum concentration. Formulations containing both types of preservatives: essential oil and a synthetic one have been tested and proposed as a compromise that allows for reducing concentration of both components due to their synergistic activity. Although most essential oils are regarded as safe, some of them may cause risk of contact allergy or phototoxic reaction. A well balanced risk-benefit assessment of essential oils is one of the great challenges for scientists or health policy authorities. This paper presents current state of knowledge on essential oils focused on their antimicrobial properties, the assessment of their efficacy and safety as cosmetic preservatives.

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“…The subpopulations may be distinguished based on different fluorescence characteristics or by light scattering, which is related to cell volume and structure and surface properties. Many fluorescent dyes have been used to analyze diverse physiological parameters, such as membrane integrity [8], activity of intracellular enzymes [9], cell vitality [10], membrane potential [11] and intracellular pH [12]. The LIVE/DEAD kit (BacLight TM , Invitrogen), which measures cell viability, has been used in combination with flow cytometry to estimate the number of viable bacteria and also the total count of bacteria [13].…”

Section: Introductionmentioning

confidence: 99%

Flow Cytometric Assessment of Lactococcus Lactis Isolates Viability after Lyophilization

Leandro¹

2014

IJNFS

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Abstract:Lactococcus lactis strains tolerance to the lyophilization process is one of the criteria used during the selection of starter cultures in cheese production. The viability of L. lactis strains isolated from silage and milk after the lyophilization process was evaluated in the present study by flow cytometry and cell plate counting. The LIVE/DEAD BacLight TM kit, which contains the dyes SYTO 9 and propidium iodide, was used to stain the cells for flow cytometry analysis. The dried cells were also plated on M17 agar and incubated at 30 °C for 24 hours. The strain L. lactis LBU.1, isolated from buffalo milk, showed the highest tolerance to the lyophilization process, according to flow cytometry and cell plate counting. Although the strains of L. lactis isolated from silage were not the most resistant to the lyophilization process, it is noteworthy that, compared to strains isolated from conventional sources such as cow and goat milk, L. lactis strains from silage showed higher tolerance to the lyophilization process.

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Flow cytometric assessment of the antimicrobial activity of essential oils against Listeria monocytogenes

Cited by 129 publications

References 50 publications

Origanum vulgare subsp. hirtum Essential Oil Prevented Biofilm Formation and Showed Antibacterial Activity against Planktonic and Sessile Bacterial Cells

Origanum vulgare subsp. hirtum Essential Oil Prevented Biofilm Formation and Showed Antibacterial Activity against Planktonic and Sessile Bacterial Cells

Application of essential oils as natural cosmetic preservatives

Flow Cytometric Assessment of Lactococcus Lactis Isolates Viability after Lyophilization

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