Flow Cytometric Analysis of the Platelet Surface Area and Surface Density of Glycoprotein IIb-IIIa of Unactivated Human Platelets of Various Sizes

Leytin, Valery; Shapiro, Hava; Новиков, Ю В; Radnay, Judith

doi:10.1006/bbrc.1996.1316

Cited by 16 publications

(16 citation statements)

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“…Overall, our theoretical and experimental data have proved that CD3 expression variability has functional implications. Opposite conclusions were raised regarding GPIIb-IIIa molecular density that was not correlated to aggregability of L platelets (41). In vitro activation of T cells was repeatedly more efficient on clonotypes with high CD3 levels compared with clonotypes with low CD3 levels.…”

Section: Variability Of Cd3 Membrane Expression and T Cell Activationmentioning

confidence: 96%

Variability of CD3 membrane expression and T cell activation capacity

Hentati

Gruy

Iobagiu

et al. 2009

Cytometry Part B Clinical

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Background: abT cells have a wide distribution of CD3 membrane density. The aim of this article was to evaluate the significance of the CD3 differential expression on T cell subsets. Analysis was performed on healthy donors and renal transplant patients by flow cytometry. The results obtained are: (1) CD3 expression was widely distributed (CV 5 38.3 6 3.1 to 43 6 2.3%). (2) The CD4, CD8, CD45 and forward scatter were similarly distributed. (3) The diversity of CD3 expression was directly related to the clonotypes: c9, non c9 from cdT cells and Vb clonotype from abT cells (e.g., Vb3FITC 7,980 6 1,628 Vb8PE: Vb20-FITC 11,768 6 1,510). (4) Using a computer simulation, we could confirm differential kinetics of T cell activation according to the initial parameters. Finally, in vitro activation was significantly higher on Vb8 and Vb9 (high CD3) compared with Vb2 and Vb3 (low CD3, P 5 0.040-0.0003).

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Section: Variability Of Cd3 Membrane Expression and T Cell Activationmentioning

confidence: 96%

Variability of CD3 membrane expression and T cell activation capacity

Hentati

Gruy

Iobagiu

et al. 2009

Cytometry Part B Clinical

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“…However, it is not fully understood which factors regulate expression of certain receptors. Flow-cytometric studies have shown that within one blood sample there is a correlation between GPIIb-IIIa and GPIb contents and platelet size [19][20][21][22]. Leytin et al [22] calculated that large platelets contained about twice more GPIIb-IIIa than small ones, whereas the density of GPIIb-IIIa per surface area unit was the same in both fractions.…”

Section: Introductionmentioning

confidence: 99%

Relationships of glycoproteins IIb-IIIa and Ib content with mean platelet volume and their genetic polymorphisms

Хаспекова

Zyuryaev

Yakushkin

et al. 2014

Blood Coagulation &Amp; Fibrinolysis

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Quantity of platelet adhesion molecules significantly varies in normal donors and cardiovascular patients and might be affected by platelet size and genetic variations. In this study, we assessed relationships of the content of glycoprotein (GP) IIb-IIIa and GPIb with mean platelet volume (MPV) and their genetic polymorphisms. MPV and GPIIb-IIIa and GPIb numbers were measured in 116 patients with acute coronary syndrome (ACS) at days 1, 3-5 and 8-12 after disease onset and in 32 healthy volunteers. GPIIb-IIIa and GPIb allelic variants were determined in ACS patients. Strong interactions of GPIIb-IIIa and GPIb numbers and MPV were observed in ACS patients and healthy volunteers. In patients, coefficients of correlation (r) were 0.642 and 0.510 (analysis of individual mean values) and in volunteers - 0.594 and 0.508 for GPIIb-IIIa and GPIb, respectively (everywhere P < 0.005). In ACS patients, correlations were highly significant at each tested time point. GPIIb-IIIa and GPIb genetic polymorphisms [GPIIIa Leu33Pro, GPIbα Thr145Met and GPIbα (-5)T/C (Kozak)] determined in ACS patients had no significant impact on their expression. Modest correlation was revealed between MPV and plasma thrombopoietin (TPO) measured at the first day of ACS (r = 0.279, P = 0.005). The data obtained indicated that GPIIb-IIIa and GPIb levels are mainly affected by platelet size (MPV) but not by their genetic variations. In some ACS patients, production of large platelets with high GPIIb-IIIa and GPIb contents might be stimulated by elevated TPO.

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“…Despite the large difference in cell size, the number of ␣ 2b ␤ 3 receptors on the platelet surface was approximately double that on the thrombocyte surface (MFI 653 on platelets vs 322 on thrombocytes; P ϭ .018; Figure 3C-D). When conservative estimates of platelet and thrombocyte diameters of 2 m and 6-7 m were used, respectively, 10 the thrombocyte was predicted to have a surface area at least 9-12 times that of a platelet, a value consistent with the difference in forward scatter observed between the 2 cell types when flow cytometry was used ( Figure 3C, Leytin et al 31 ). Thus, the density of ␣ 2b ␤ 3 receptors on platelets was at least 18-25 times greater than that on thrombocytes.…”

Section: Occlusive Thrombi In Mammals But Not Birds 3665mentioning

confidence: 73%