Flow cytometric analysis of T-lymphocyte subpopulations in B-cell chronic lymphocytic leukemia: Correlation with clinical stage

Hautekeete, M. L.; Bock, Rudolf; Bockstaele, Dirk R. Van; Colpin, G. C.; Berneman, Zwi; Peetermans, M.

doi:10.1007/bf00367462

Cited by 9 publications

(2 citation statements)

References 17 publications

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“…2% for WBC and WBC differentials and 1.3% for Ht. Flow cytometric evaluation of the different lymphocyte subsets was carried out on 50 Ìl of fresh, total blood after NH 4 Cl lysis of the RBC, and using the same batches of directly conjugated antisera througout the study span [20]. All antisera were purchased from Beckton Dickinson (Erenbodegem, Belgium) except for the CD26 antibody [21].…”

Section: Methodsmentioning

confidence: 99%

The Effects of Psychological Stress on Leukocyte Subset Distribution in Humans: Evidence of Immune Activation

et al. 1999

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The aim of the present study was to examine the effects of academic examination stress on leukocyte subset distribution in university students. Thirty-eight university students had repeated blood collections for white blood cell differentiation and flow cytometric assay of lymphocytic subsets a few weeks before and after (i.e. two baseline conditions) as well as the day before a difficult academic examination (i.e. stress condition). Flow cytometry was used to determine the number of peripheral blood mononuclear cells (PBMC). In students, who were reactors to psychological stress (criterion based on changes in the Perceived Stress Scale, PSS), but not in stress non-reactors, a significant increase in the number of neutrophils, monocytes, CD8⁺, CD2⁺CD26⁺, and CD2⁺HLA-DR⁺ T cells and CD19⁺ B cells, and significant reductions in the CD4⁺/CD8⁺ T cell ratio were observed in the stress condition. There were significant and positive relationships between the stress-induced changes in perceived stress (PSS scale) and number of leukocytes, neutrophils, CD2⁺, CD2⁺CD26⁺ and CD2⁺HLADR⁺ T cells, and CD19⁺ B cells. There were significant and negative relationships between the stress-induced changes in the CD4⁺/CD8⁺ ratio and the stress-induced changes in the PSS scale. Female students taking oral contraceptives showed significantly higher stress-induced responses in number of leukocytes, neutrophils and CD19⁺ B cells than male and female students without use of oral contraceptives. The results suggest that academic examination stress induces changes in the distribution of PBMC, which indicate immune activation and which are probably orchestrated by a stress-induced production of cytokines.

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Section: Methodsmentioning

confidence: 99%

The Effects of Psychological Stress on Leukocyte Subset Distribution in Humans: Evidence of Immune Activation

et al. 1999

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“…Both CD4+ and CD8+ subpopulations decrease substantially, but CD4+ reduction is more substantial, resulting in a reversal of the CD4+:CD8+ ratio. This phenomenon is also seen in advanced stages of chronic lymphocytic leukemia and in HIV-infected patients (6). The reduction of CD4+ lymphocytes and the reversal of the CD4+:CD8+ ratio is thought to result in increased susceptibility to opportunistic infections.…”

Section: Cryptococcal Meningitis Following Fludarabine Treatment For mentioning

confidence: 88%

Cryptococcal meningitis following fludarabine treatment for chronic lymphocytic leukemia

Leenders

Sonneveld

Marie

1995

Eur. J. Clin. Microbiol. Infect. Dis.

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Clinical and immunological evaluation of primary splenic irradiation in chronic lymphocytic leukemia: a study of 24 cases

Mook

Fickers

Verschueren³

2001

Ann Hematol

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Little is known about the effects of primary splenic irradiation (SI) in B-cell chronic lymphocytic leukemia (B-CLL) on subpopulations of lymphocytes, immunoglobulins, and the induction of autoantibodies against erythrocytes and platelets. Twenty-four untreated patients with B-CLL were studied prospectively. One patient was excluded from analysis because of intercurrent death. Of the remaining 23 patients, 7 were female and 16 were male, with an average age of 68.4 and 61.2 years, respectively. Treatment consisted of a weekly dose of I Gy up to a total dose of 10 Gy to the spleen. Standardized evaluation was done 2 weeks before and 6 weeks after SI. Twenty patients completed the course of SI. In 14 patients there was a partial response and in 9 patients the disease remained stable. The number of leukocytes decreased rapidly and significantly, with a decrease in the fraction of lymphocytes and an increase in the neutrophil count (all P<0.0001). There was a significant increase in platelet count, but not in hemoglobin level. Nausea, slight diarrhea, pleuropneumonia, granulopenia with fever (all n=1), urinary tract infection and high fever (n=1), and thrombocytopenia occurred (n=2). One patient received transfusion of packed cells because of upper digestive tract bleeding for which cessation of SI was not necessary. The number of malignant cells showed a significant decrease (P<0.01). No significant changes in CD4/CD8 ratio were found. The number of CD4+ and CD8+-cells, however, decreased significantly (P<0.01 and 0.03, respectively). DAT remained unchanged in most (93%) and tests for antibodies to platelets in 83% of the patients. No significant changes in immunoglobin levels were observed.

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Flow cytometric analysis of T-lymphocyte subpopulations in B-cell chronic lymphocytic leukemia: Correlation with clinical stage

Cited by 9 publications

References 17 publications

The Effects of Psychological Stress on Leukocyte Subset Distribution in Humans: Evidence of Immune Activation

The Effects of Psychological Stress on Leukocyte Subset Distribution in Humans: Evidence of Immune Activation

Cryptococcal meningitis following fludarabine treatment for chronic lymphocytic leukemia

Clinical and immunological evaluation of primary splenic irradiation in chronic lymphocytic leukemia: a study of 24 cases

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