Flow cytometric analysis of peroxidase negative acute leukemias by monoclonal antibodies—II. Acute megakaryoblastic and acute promegakaryocytic leukemias

Imamura, Nobutaka; Kajihara, Hiroki; Kuramoto, Atsushi

doi:10.1016/0145-2126(88)90042-2

Cited by 29 publications

(3 citation statements)

References 41 publications

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“…However, our data clearly demonstrate that AML and AMMoL were included among unclassifiable leukemias, and were categorized as ALL by FAB classification, in spite of expressing OKM1-(9) or Mol (17) (CD 11)-reactive antigens on cell surfaces. In addition, we identified 3 cases of acute megakaryoblastic leukemia by means of several monoclonal antibodies (21)(22)(23) for megakaryocyte-platelet-related surface antigens and ultrastructural cytochemistry for PPO activity (24). The specific monoclonal antibodies used were 5F1, AN51 (GPIb) (21), J15, P2 (GP IIb/IIIa) (22) and Plt-1 (23).…”

Section: Discussionmentioning

confidence: 99%

Analysis of peroxidase‐negative acute unclassifiable leukemias by monoclonal antibodies. 1. Acute myelogenous leukemia and acute myelomonocytic leukemia

Imamura

Tanaka

Kajihara

et al. 1988

European J of Haematology

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In this study, pretreatment peripheral and/or bone marrow blasts from 12 patients with acute unclassifiable leukemia (AUL) expressing the myeloid‐related cell‐surface antigen (CD 11) were isolated for further analysis. Despite a lack of myeloperoxidase (MPO) activity, 1 patient's blasts contained cytoplasmic Auer rods. The circulating blasts from another patient expressed MPO while maintaining the same surface phenotype during 20 months of clinical follow‐up. In addition, the blasts from 3 cases demonstrated both myelomonocytic and monocyte‐specific surface antigens, whereas the remaining 9 cases completely lacked any monocyte‐specific antigen detectable by monoclonal antibodies, Mo2, My4 and Leu M3 (CD 14). The first case eventually was diagnosed as acute myelomonocytic leukemia and the second as acute myelogenous leukemia by means of immunophenotypic analysis using flow cytometry (FACS IV). In addition, the presence of MPO protein was identified in the cytoplasm of blast cells from 5 patients with AUL by means of a cytoplasmic immunofluorescence test using a monoclonal antibody (MA1). Our study indicates that non‐T, non‐B AUL expressing OKM1 (CD 11) antigens include acute leukemias which are unequivocally identifiable as being of either myeloid or myelomonocytic origin. However, further investigations, including immunophenotypic and cytoplasmic analysis, ultrastructural cytochemistry and gene analysis with molecular probes (tests applicable to normal myeloid cells), are necessary in order to determine the actual origin of blasts and to recognize the differentiation stages of the various types of leukemic cells from patients with undifferentiated forms of leukemia.

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Section: Discussionmentioning

confidence: 99%

Analysis of peroxidase‐negative acute unclassifiable leukemias by monoclonal antibodies. 1. Acute myelogenous leukemia and acute myelomonocytic leukemia

Imamura

Tanaka

Kajihara

et al. 1988

European J of Haematology

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“…(Tokyo) and Japan Scientific Instruments (Tokyo). Lymphocyte cell-surface antigens were analysed by means of a fluorescence-activated cell sorter (FACS IV), utilizing a panel of monoclonal antibodies as described previously (Imamura et al 1985(Imamura et al . 1988.…”

Section: T-cell Lymphocytic Leukaemia With the Cd4+ Cd45rf Cd29-surmentioning

confidence: 99%

T‐CELL LYMPHOCYTIC LEUKAEMIA WITH THE CD4⁺, CD45R⁺, CD29‐ SURFACE PHENOTYPE FUNCTIONED AS T SUPPRESSOR INDUCER FOR B‐CELL IMMUNOGLOBULIN SYNTHESIS

1989

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“…Cell surface antigens were analyzed by a fluorescent-activated cell sorter (FACS IV; Becton Dickinson, Mountain View, CA) using a panel of MAbs as previously described [6]. Immunophenotypically , the differentiation stage for the MEG-01 cell line was considered to belong the megakaryoblast, which was demonstrated by both the presence of the megakaryocyte-platelet-related cell surface antigens GP IIb/IIIa (J15, P2, HPL2, and HPLS), Plt-1, OKM6, and Ia (HLA-DR), and the lack of GP Ib (5F1 and AN51) [6]. The antigen expression detected by OKM5 MAb was also clearly found on the surface of this cells.…”

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confidence: 99%

Letter to the editor: Demonstration of tsp receptor both on the cell surface and in the cytoplasm of the megakaryoblastic leukemia cell line (MEG‐01)

et al. 1989

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Thrombospondin (TSP), is an adhesive 450-kD multifunctional glycoprotein composed of three identical disulphide-linked chains [l]. TSP has a broad tissue distribution, is synthesized by endothelial cells, fibroblasts, smooth muscle cells, alveolar pneumocytes, monocytes, and neuroglial cells, and is known to bind heparin, fibrinogen, fibronectin, histidine-rich glycoprotein, collagen, and plasminogen.OKM5 monoclonal antibody (CD36) is reactive with platelets, endothelial cells, and monocytes [2]. It identifies an 88-kD glycoprotein and has recently been found to inhibit the specific binding of TSP to C32 melanoma cells and HT1080 fibrosarcoma cells and to react with the cellular receptor for TSP [ 3 ] . The expression of OKM5 antigen (TSP receptor) on the cell surface of megakaryoblastic leukemia has been reported previously [4]. We describe here the existence of TSP receptor on the cell surface and in the cytoplasm of the megakaryoblastic cell line MEG-01 [5]. Analysis of the culture cells (MEG-01) by means of indirect immunofluorescence technique is described. Monoclonal antibodies (MAbs) were obtained through the courtesy of Drs. A.J. McMichael, H. Shiku, and G.Goldstein, and Ortho Diagnostic Systems K.K. and the Japan Scientific Instrument Company. Cell surface antigens were analyzed by a fluorescent-activated cell sorter (FACS IV; Becton Dickinson, Mountain View, CA) using a panel of MAbs as previously described [6]. Immunophenotypically , the differentiation stage for the MEG-01 cell line was considered to belong the megakaryoblast, which was demonstrated by both the presence of the megakaryocyte-platelet-related cell surface antigens GP IIb/IIIa (J15, P2, HPL2, and HPLS), Plt-1, OKM6, and Ia (HLA-DR), and the lack of GP Ib (5F1 and AN51) [6]. The antigen expression detected by OKM5 MAb was also clearly found on the surface of this cells. In addition, OKM5 antigen increased while culturing the MEG-01 cells with phorbol diesters and was also found in the cytoplasm by means of the indirect cytoplasmic immunofluorescence test [ 7 ] . (Fig. 1). On the basis of these findings, we conclude that TSP receptor certainly exists both on the cell surface and in the cytoplasm of the megakaryoblastic cell line (MEG-01). REFERENCESI . Lawler J, Denck LH, Connolly JE, Chen JH, Chao FC: The stnicture of human platelet thrombospondin. J Biol Chem 260:3762, 19x5. 2. Talk MA, Rao PE, Westberg E, Allegan N, Makowski M, Mittler RS, Goldstein G: Patterns of antigenic expression on human monocytcs as defined by monoclonal antibodies. Cell lmmunol 78:83, 1983. 3. Asch AS, Barnwell J , Silverstein RL, Nachman RL: Isolation of the thrombospondin membrane receptor. J Clin lnvest 79: 1054, 1987. 4. Imamura N, lnada T, Mtasiwa DM, Kuramoto, A: Demonstration of TSP receptor on the cell surface of acute megakaryoblastic leukemia Am J

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Flow cytometric analysis of peroxidase negative acute leukemias by monoclonal antibodies—II. Acute megakaryoblastic and acute promegakaryocytic leukemias

Cited by 29 publications

References 41 publications

Analysis of peroxidase‐negative acute unclassifiable leukemias by monoclonal antibodies. 1. Acute myelogenous leukemia and acute myelomonocytic leukemia

Analysis of peroxidase‐negative acute unclassifiable leukemias by monoclonal antibodies. 1. Acute myelogenous leukemia and acute myelomonocytic leukemia

T‐CELL LYMPHOCYTIC LEUKAEMIA WITH THE CD4⁺, CD45R⁺, CD29‐ SURFACE PHENOTYPE FUNCTIONED AS T SUPPRESSOR INDUCER FOR B‐CELL IMMUNOGLOBULIN SYNTHESIS

Letter to the editor: Demonstration of tsp receptor both on the cell surface and in the cytoplasm of the megakaryoblastic leukemia cell line (MEG‐01)

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Flow cytometric analysis of peroxidase negative acute leukemias by monoclonal antibodies—II. Acute megakaryoblastic and acute promegakaryocytic leukemias

Cited by 29 publications

References 41 publications

Analysis of peroxidase‐negative acute unclassifiable leukemias by monoclonal antibodies. 1. Acute myelogenous leukemia and acute myelomonocytic leukemia

Analysis of peroxidase‐negative acute unclassifiable leukemias by monoclonal antibodies. 1. Acute myelogenous leukemia and acute myelomonocytic leukemia

T‐CELL LYMPHOCYTIC LEUKAEMIA WITH THE CD4+, CD45R+, CD29‐ SURFACE PHENOTYPE FUNCTIONED AS T SUPPRESSOR INDUCER FOR B‐CELL IMMUNOGLOBULIN SYNTHESIS

Letter to the editor: Demonstration of tsp receptor both on the cell surface and in the cytoplasm of the megakaryoblastic leukemia cell line (MEG‐01)

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T‐CELL LYMPHOCYTIC LEUKAEMIA WITH THE CD4⁺, CD45R⁺, CD29‐ SURFACE PHENOTYPE FUNCTIONED AS T SUPPRESSOR INDUCER FOR B‐CELL IMMUNOGLOBULIN SYNTHESIS