FLOW CYTOMETRIC ANALYSIS OF NUCLEAR DNA BETWEEN OKRA LANDRACES (<i>ABELMOSCHUS ESCULENTUS</i> L.)

Abstract

Okra Abelmoschus esculentus L. (Moench), is an economically important vegetable crop grown in tropical and sub-tropical as well as Mediterranean countries. The genome size of the Abelmoschus genus species is still rare. Ploidy determinations have traditionally been done by counting chromosomes of stained root tips, but this method is laborious and often difficult with species which have small chromosomes and high ploidy levels and can lead to misclassified. Flow cytometry has been widely used in various aspects of plant research, such as the determination of the DNA nuclear content. Nuclear DNA size of fifteen okra genotype was measured using flow cytometry. The data showed variation between Turkish genotype and Jordanian genotype. Genome size of okra ranged from 3.98 pg 2C −1 in Jordanian landrace to 17.67pg 2C −1 in Turkish landrace. The 2C genome size in Mbp ranged from 3897-17321 among Abelmoschus esculentus genotypes. Further studies is recommended to accurate chromosome count to be linked with the genome size.

Genome and transcriptome architecture of allopolyploid okra (Abelmoschus esculentus)

“…Furthermore, correct de novo assembly is presumed to be complex because of the expected large genome and transcriptome size and the highly polyploid nature of the genome. Salameh (2014) reported flow-cytometric estimates of nuclear DNA size estimations with 2C values ranging from 3.98 to 17.67 pg, equaling to genome sizes between 3.8 to 17.3 Gbp. In addition, chromosome counts demonstrated a huge variation, ranging from 2n=62 to 2n=144, with 2n=130 as the most frequently observed chromosome number (Benchasri, 2012; Merita et al ., 2012).…”

GENETIC DIVERSITY OF OKRA (<i>ABELMOSCHUS ESCULENTUS</i> L.) GENOTYPES FROM DIFFERENT AGRO-ECOLOGICAL REGIONS REVEALED BY AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS

“…Phenotypic variation has been studied by several studies (Salameh and Kasrawi, 2011;Bello et al, 2006;Düzyaman, 2006;Ghai et al, 2005;Rawashdeh, 1999;Ariyo, 1987). Nuclear DNA content of okra using Flow Cytometry has been measured to be ranged between 3897-17321 Mpb (Salameh, 2014).…”

Characterization of Okra Species, Their Hybrids and Crossability Relationships among Abelmoschus Species of the Western Ghats Region

“…The species included in the crossability studies affects the success of interspecific hybridization between domesticated okra and their wild counterparts. Differences in the ploidy level among the okra species used in this study might have contributed to the success or failure of hybridization [36,37]. Colchicine treatment of the seedlings was effective in overcoming the sterility in the interspecific F 1 hybrid plants, resulting in partially fertile amphidiploids.…”