Flow cytometric analysis for detection of tumor-initiating cells in feline mammary carcinoma cell lines

Michishita, Masaki; Otsuka, Aya; Nakahira, Rei; Nakagawa, Takayuki; Sasaki, Nobuo; Arai, Toshiro; Takahashi, Koji

doi:10.1016/j.vetimm.2013.08.006

Cited by 7 publications

(10 citation statements)

References 33 publications

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“…Feline mammary CSC formed tumorspheres that were serially passaged, demonstrating self‐renewal capability . Xenotransplantation of feline mammary CSC into NOD/SCID mice resulted in tumor development, demonstrating their ability to drive tumor formation and growth . The notice that CSC in canine and feline are comparable to CSC described in human breast cancer re‐emphasizes the translational value of feline and canine mammary cancer as a model for human breast cancer.…”

Section: Characterization Of Mammary Stem Cellsmentioning

confidence: 81%

“…The expression profile of this CMT‐U229 Sca‐1 + SP was reported as being CD10 lo /CD34 + /CD44 + /CD24 lo /CD49f + . Like their canine and human counterparts, feline mammary CSC expressed CD24 – CD44 + ALDH + , and CD44 expression decreased after the cells differentiated in vitro . Feline mammary CSC formed tumorspheres that were serially passaged, demonstrating self‐renewal capability .…”

Section: Characterization Of Mammary Stem Cellsmentioning

confidence: 93%

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Conserved and variable: Understanding mammary stem cells across species

2017

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Postnatal mammary gland development requires the presence of mammary stem and progenitor cells (MaSC), which give rise to functional milk-secreting cells and regenerate the mammary epithelium with each cycle of lactation. These long-lived, tissue-resident MaSC are also targets for malignant transformation and may be cancer cells-oforigin. Consequently, MaSC are extensively researched in relation to their role and function in development, tissue regeneration, lactation, and breast cancer. The basic structure and function of the mammary gland are conserved among all mammalian species, from the most primitive to the most evolved. However, species vary greatly in their lactation strategies and mammary cancer incidence, making MaSC an interesting focus for comparative research. MaSC have been characterized in mice, to a lesser degree in humans, and to an even lesser degree in few additional mammals. They remain uncharacterized in most mammalian species, including "ancient" monotremes, marsupials, wild, and rare species, as well as in common and domestic species such as cats. Identification and comparison of MaSC across a large variety of species, particularly those with extreme lactational adaptations or low mammary cancer incidence, is expected to deepen our understanding of development and malignancy in the mammary gland. Here, we review the current status of MaSC characterization across species, and underline species variations in lactation and mammary cancer through which we may learn about the role of MaSC in these processes. V C 2017 International Society for Advancement of Cytometry

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Section: Characterization Of Mammary Stem Cellsmentioning

confidence: 81%

Section: Characterization Of Mammary Stem Cellsmentioning

confidence: 93%

Conserved and variable: Understanding mammary stem cells across species

2017

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“…Many previous studies that had been performed on companion animals have reported the identification and isolation of BCSCs. In felines and canines, TICs were identified and isolated by mammosphere formation, decrease ALDH1 enzyme activity, or cell surface markers (CD44, CD24, and CD133) either from spontaneous mammary carcinoma (Barbieri et al, 2012(Barbieri et al, , 2015 or mammary carcinoma cell lines (Michishita et al, 2013;Pang et al, 2013).…”

mentioning

confidence: 99%

“…In veterinary oncology, M. Michishita and coworkers (Michishita et al, 2013) were the first to use the FCA to recognize TICs in feline mammary carcinoma cell lines. Also, the diagnostic precision of FCA to discriminate neoplastic from non-neoplastic lymphoproliferative anarchies in cats was reported in a study by Martini and colleagues (Martini et al, 2018).…”

mentioning

confidence: 99%

Quantitative Flow Cytometry Assessment of Feline Circulatory Breast Cancer Stem Cells

Elsabagh¹,

Farghali²,

Emam³

et al. 2021

AAVS

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S pontaneous mammary tumors are one of the most common malignancies in cats. Almost 90% of the feline mammary carcinomas (FMC) are malignant, and they tend to metastasized and spread to the lungs and lymph nodes (Karabolovski et al., 2015). The WHO proposed female cats with naturally developed mammary tumors as an excellent model for studying breast carcinoma in human neoplasia. As feline shares many epidemiological, clinical, and morphological similarities with human breast cancer besides their shorter lifespan and the faster progression that provides a quicker gathering of data and trial accomplishment (De Las Mulas et al., 2000; Cannon, 2015).The diagnosis, prognosis, and monitoring of therapeutic antitumor activity are mainly dependent on immunological examination or pathological features with histopathological investigations (Zappulli et al., 2015). The former techniques require repeatable tumor tissue biopsies for research Article Abstract | The upsurge need for a rapid and accurate prognostic system for feline mammary neoplasia is crucial, as almost 90% of mammary tumors in cats tend to metastasize. Here, appears the importance of circulatory cancer biomarkers (liquid biopsies) such as circulating tumor cells (CTCs) and their subpopulation circulating cancer stem cells (cCSCs) as an effective, feasible, repeatable, and non-invasive tool for tumor prognosis and treatment monitoring.In the current prototype study, we detected and enumerated the circulatory breast cancer stem cells (BCSCs) for the first time in the feline peripheral blood by using the flow cytometer analysis (FCA) against their specific cellular markers (CD44 + /CD24and CD133 + ). In addition, we calculated Area Under the Curve (AUC), like hood ratios and cutoff points by statistical analysis. The results revealed statistically significant differences (p <0.0001) between healthy and diseased animal groups with an excellent AUC value (0.902 for CD44 + /CD24cells and 0.990 for CD133 + population) that supported the use of FCA as a sensitive, specific, and rapid diagnostic and monitoring tool for mammary carcinoma. We also set up a cutoff value of diagnostic significance (>276 for CD44 + /CD24populations and >12 for CD133 + cells).

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“…Ab clones and fluorochromes (Table 1) have already been documented to stain feline samples by FC. 3,9,10 All antibodies, as well as PI, had been titered before use to determine the best working dilutions. All samples were evaluated immediately after staining by means of a flow cytometer (BriCyte E6; Mindray) equipped with 2 lasers and detectors for up to 4 fluorescence channels.…”

mentioning

confidence: 99%

Flow cytometry expression pattern of CD44 and CD18 markers on feline leukocytes

Martini

Bernardi²,

Giordano³

et al. 2020

J VET Diagn Invest

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The paucity of specific feline antibodies for flow cytometry (FC) is an ongoing challenge. Flow cytometrists must extrapolate information from relatively few markers. We evaluated the expression pattern of the panleukocyte markers CD18 and CD44 on leukocyte (white blood cell, WBC) subclasses in the peripheral blood (PB) of 14 healthy cats. The degree of expression of CD18 and CD44 was calculated as the ratio between the median fluorescence intensity (MFI) value of antibody-stained cells and autofluorescence. All samples were acquired with the same cytometer with constant photomultiplier setting and compensation matrices. Both molecules were expressed at higher levels on monocytes, intermediate levels on polymorphonuclear cells (PMNs), and lower levels on lymphocytes. CD18-MFI discriminated well among the 3 populations, whereas CD44-MFI mostly overlapped between monocytes and PMNs. However, CD44-MFI had a lower intra-population variability. Evaluation of CD18 and CD44, together with morphologic parameters, was useful for discriminating among WBC subclasses in healthy cats. This information may be helpful for future studies given that an increase in CD18-MFI may indicate reactive changes, whereas fluctuations in CD44-MFI may suggest neoplasia.

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Flow cytometric analysis for detection of tumor-initiating cells in feline mammary carcinoma cell lines

Cited by 7 publications

References 33 publications

Conserved and variable: Understanding mammary stem cells across species

Conserved and variable: Understanding mammary stem cells across species

Quantitative Flow Cytometry Assessment of Feline Circulatory Breast Cancer Stem Cells

Flow cytometry expression pattern of CD44 and CD18 markers on feline leukocytes

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