Flow cells as<i>quasi</i>-ideal systems for biofouling simulation of industrial piping systems

Teodósio, Joana; Silva, Filipe; Moreira, Joana; Simões, Manuel; Melo, L. F.; Alves, M.A.; Mergulhão, Filipe

doi:10.1080/08927014.2013.821467

Cited by 29 publications

(23 citation statements)

References 37 publications

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“…Additionally, the user must have prior knowledge of the flow dynamics inside the device in order to make sure that the flow is completely developed in the area where the coupons are located. Entry effects are common on these devices and therefore a stabilization length is required to allow direct comparison of the biofilm obtained in different coupons (Teodosio et al, 2013b). However, the versions of the Robbins device have the advantage that they can sustain continued biofilm growth for several weeks or more without interruption (Manz et al, 1993;Teodosio et al, 2011Teodosio et al, , 2012.…”

Section: Robbins Devicementioning

confidence: 99%

Critical review on biofilm methods

Azeredo

Azevedo

Briandet

et al. 2016

Critical Reviews in Microbiology

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736

679

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Biofilms are widespread in nature and constitute an important strategy implemented by microorganisms to survive in sometimes harsh environmental conditions. They can be beneficial or have a negative impact particularly when formed in industrial settings or on medical devices. As such, research into the formation and elimination of biofilms is important for many disciplines. Several new methodologies have been recently developed for, or adapted to, biofilm studies that have contributed to deeper knowledge on biofilm physiology, structure and composition. In this review, traditional and cutting-edge methods to study biofilm biomass, viability, structure, composition and physiology are addressed. Moreover, as there is a lack of consensus among the diversity of techniques used to grow and study biofilms. This review intends to remedy this, by giving a critical perspective, highlighting the advantages and limitations of several methods. Accordingly, this review aims at helping scientists in finding the most appropriate and up-to-date methods to study their biofilms. ARTICLE HISTORY

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Section: Robbins Devicementioning

confidence: 99%

Critical review on biofilm methods

Azeredo

Azevedo

Briandet

et al. 2016

Critical Reviews in Microbiology

Self Cite

736

679

View full text Add to dashboard Cite

show abstract

“…Escherichia coli strain JM109 (DE3) was used as a model bacterium at laboratory scale, as it has been routinely used for biofilm research in previous works (Moreira et al, 2015;Teodósio et al, 2013) (1 × 10 6 cells mL −1 ) in the appropriate growth medium were prepared.…”

Section: Strain and Culture Conditionsmentioning

confidence: 99%

Disinfection with neutral electrolyzed oxidizing water to reduce microbial load and to prevent biofilm regrowth in the processing of fresh-cut vegetables

Machado

Meireles

Fulgêncio

et al. 2016

Food and Bioproducts Processing

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Product decontamination is one of the most important processes of the hygienic practice in food industries such as Minimally Processed Vegetables (MPV) plants and sodium hypochlorite (NaOCl) solutions are commonly used as a biocide for disinfection. Although it may be corrosive and irritating when compared to alternative biocides, this biocide is frequently applied at high concentrations. This work aims at studying the use of lower concentrations of chlorine by testing neutral electrolyzed oxidizing water (NEOW) as a chlorine-source disinfectant in fresh-cut salad processing. Assays were performed at industrial and laboratory scale. Results showed that lower doses of chlorine from NEOW (30 ppm) are as effective as higher concentrations of the traditional chlorine from NaOCl (80 ppm) in the reduction of total microbial population at industrial scale. Moreover, in laboratory studies, the NEOW chlorine was also more effective in biofilm eradication, as well as a biofilm preventive agent. NEOW can thus be a successful alternative water disinfection technique, reducing the free chlorine concentration needed to sanitize salads, also decreasing water consumption whilst taking into account environmental and food quality impacts.

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“…In order to translate the experimental results reported here to real systems, it is important to ensure that the same shear stresses (0 or 0.27 Pa) are being generated in the industrial lines since it has been shown that shear stress is a good scale-up parameter between different systems (Moreira et al, 2015;Teodósio et al, 2013). Fig.…”

Section: Resultsmentioning

confidence: 99%

The impact of material properties, nutrient load and shear stress on biofouling in food industries

Moreira

Gomes

Simões

et al. 2015

Food and Bioproducts Processing

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Abstract:In the food industry, biofilm formation in pipes, equipment and cooling systems increases maintenance costs, decreases operational efficiencies and is a source of contamination.Shear stress, nutrient load and surface material are important variables affecting the biofilm onset in industry. In this work, the combined impacts of these variables were assessed using three different materials (glass, copper and stainless steel), two nutrient loads (high and low nutrient medium) and two hydrodynamic conditions (static and dynamic). Initial adhesion and biofilm formation were studied in microplates usingEscherichia coli as a model organism.Surface material was the factor with the strongest impact and adhesion/biofilm formation were correlated with surface hydrophobicity. However, the impact of this variable was dependent on the nutrient load and imposed shear stress. It was also found that, for the majority of the situations tested, initial attachment performance is a good predictor of biofilm formation behaviour and that the effects observed during attachment are amplified during biofilm maturation. Since shear stress is a major determinant in cell adhesion, the results of this study may find application in industrial systems operating at flow rates between 0.001 and 600 m 3 h -1 depending on tube material and diameter.

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Flow cells asquasi-ideal systems for biofouling simulation of industrial piping systems

Cited by 29 publications

References 37 publications

Critical review on biofilm methods

Critical review on biofilm methods

Disinfection with neutral electrolyzed oxidizing water to reduce microbial load and to prevent biofilm regrowth in the processing of fresh-cut vegetables

The impact of material properties, nutrient load and shear stress on biofouling in food industries

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