Floral pigmentation pattern in Oriental hybrid lily ( Lilium spp.) cultivar ‘Dizzy’ is caused by transcriptional regulation of anthocyanin biosynthesis genes

Yamagishi, Masumi; Uchiyama, Hirohide; Handa, Takashi

doi:10.1016/j.jplph.2018.05.008

Cited by 26 publications

(11 citation statements)

References 61 publications

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“…In addition to species with uniformly colored flowers, many plants have flowers with contrasting color elements that create particular patterns. To date, genetic evaluation of floral pigmentation patterning has focused primarily on venation (pigment stripes associated with veins) (Schwinn et al, 2006;Albert et al, 2011;Shang et al, 2011;Hsu et al, 2015) and spot formation (Chiou & Yeh, 2008;Yamagishi et al, 2010Yamagishi et al, , 2014Yamagishi et al, , 2018Martins et al, 2013Martins et al, , 2017Yuan et al, 2014;Hsu et al, 2015;Yamagishi, 2018). These studies show a striking similarity: R2R3-MYB transcription factors are important in regulating these patterns.…”

Section: Introductionmentioning

confidence: 99%

R2R3‐MYB genes control petal pigmentation patterning in Clarkia gracilis ssp. sonomensis (Onagraceae)

Lin

Rausher

2020

New Phytologist

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Petal pigmentation patterning is widespread in flowering plants. The genetics of these pattern elements has been of great interest for understanding the evolution of phenotypic diversification. Here, we investigate the genetic changes responsible for the evolution of an unpigmented petal element on a colored background. We used transcriptome analysis, gene expression assays, cosegregation in F 2 plants and functional tests to identify the gene(s) involved in petal coloration in Clarkia gracilis ssp. sonomensis. We identified an R2R3-MYB transcription factor (CgsMYB12) responsible for anthocyanin pigmentation of the basal region ('cup') in the petal of C. gracilis ssp. sonomensis. A functional mutation in CgsMYB12 creates a white cup on a pink petal background. Additionally, we found that two R2R3-MYB genes (CgsMYB6 and CgsMYB11) are also involved in petal background pigmentation. Each of these three R2R3-MYB genes exhibits a different spatiotemporal expression pattern. The functionality of these R2R3-MYB genes was confirmed through stable transformation of Arabidopsis. Distinct spatial patterns of R2R3-MYB expression have created the possibility that pigmentation in different sections of the petal can evolve independently. This finding suggests that recent gene duplication has been central to the evolution of petal pigmentation patterning in C. gracilis ssp. sonomensis.

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Section: Introductionmentioning

confidence: 99%

R2R3‐MYB genes control petal pigmentation patterning in Clarkia gracilis ssp. sonomensis (Onagraceae)

Lin

Rausher

2020

New Phytologist

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“…In lilies, some of the TFs that regulate anthocyanin biosynthesis have been previously described; among which, R2R3-MYBs are the most extensively studied. Lilium has several R2R3-MYB TFs with different expression profiles, which contribute to a diverse range of color patterns in the flower tepals (Yamagishi et al, 2018). Lilium MYB12 (LhMYB12) generally regulates the synthesis of anthocyanins in the whole tepals in Asiatic hybrids (Yamagishi et al, 2010(Yamagishi et al, , 2012 and Oriental hybrids (Yamagishi, 2011).…”

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confidence: 99%

“…Lilium MYB12 (LhMYB12) generally regulates the synthesis of anthocyanins in the whole tepals in Asiatic hybrids (Yamagishi et al, 2010(Yamagishi et al, , 2012 and Oriental hybrids (Yamagishi, 2011). LhMYB12-Lat regulates the development of splatter-type spots in the Tango Series cultivars of Asiatic hybrid lilies (Yamagishi et al, 2014b), whereas Lilium regale MYB15 (Lr MYB15) is related to the bud-blush pigmentation (Yamagishi, 2016), and LhMYB18 is associated with the big-spot pigmentation in Asiatic hybrid lilies (Yamagishi et al, 2018). Two bHLH-type TFs have been found in lily, and LhbHLH2 is directly related to anthocyanin synthesis (Nakatsuka et al, 2009).…”

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confidence: 99%

Cloning and Characterization of a Tryptophan–Aspartic Acid Repeat Gene Associated with the Regulation of Anthocyanin Biosynthesis in Oriental Hybrid Lily

Dou¹,

Bai²,

Wang³

et al. 2020

J. Amer. Soc. Hort. Sci.

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Anthocyanins are major pigments responsible for the color of lily (Lilium sp.) flowers. Anthocyanin synthesis is part of the flavonoid metabolic pathway. Numerous transcription factors, including R2R3-MYBs, basic helix-loop-helix (bHLH), and tryptophan–aspartic acid repeat (also known as WD40 or WD repeat) proteins, known to regulate flavonoid biosynthesis have been identified in various plant species. However, there is limited information available on WD repeat proteins in lilies. In this study, we identified a WD repeat gene in the Oriental hybrid lily ‘Sorbonne’ (Lilium hybrid WD repeat, LhWDR). LhWDR contains no introns, and has a 1100–base pair open reading frame, encoding a putative protein of 370 amino acids. LhWDR was found to be localized in the cytoplasm of transgenic Arabidopsis thaliana root cells. Expression patterns of LhWDR in different organs and at different periods of lily tepal growth revealed that the expression levels of this gene are closely associated with anthocyanin accumulation. A yeast two-hybrid assay demonstrated that full-length LhWDR interacts with the 420 N-terminal amino acids of Lilium hybrid bHLH2. Interestingly, overexpression of LhWDR in A. thaliana led to an upregulation of the dihydroflavonol 4-reductase gene, which is an important structural gene downstream of the anthocyanin pathway. These results indicate that the WD repeat protein LhWDR might interact with a bHLH transcription factor to regulate anthocyanin biosynthesis.

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“…In contrast, overexpression of MYB12 in seedlings led to an increased expression of CHS , CHI , F3H and FLS [51]. MYB factors have also been demonstrated to positively regulate the expression of EBGs in other species [1, 21, 79, 89].…”

Section: Discussionmentioning

confidence: 99%

Hybrid de novo transcriptome assembly of poinsettia (Euphorbia pulcherrima Willd. Ex Klotsch) bracts

et al. 2019

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BackgroundPoinsettia is a popular and important ornamental crop, mostly during the Christmas season. Its bract coloration ranges from pink/red to creamy/white shades. Despite its ornamental value, there is a lack of knowledge about the genetics and molecular biology of poinsettia, especially on the mechanisms of color formation. We performed an RNA-Seq analysis in order to shed light on the transcriptome of poinsettia bracts. Moreover, we analyzed the transcriptome differences of red- and white-bracted poinsettia varieties during bract development and coloration. For the assembly of a bract transcriptome, two paired-end cDNA libraries from a red and white poinsettia pair were sequenced with the Illumina technology, and one library from a red-bracted variety was used for PacBio sequencing. Both short and long reads were assembled using a hybrid de novo strategy. Samples of red- and white-bracted poinsettias were sequenced and comparatively analyzed in three color developmental stages in order to understand the mechanisms of color formation and accumulation in the species.ResultsThe final transcriptome contains 288,524 contigs, with 33% showing confident protein annotation against the TAIR10 database. The BUSCO pipeline, which is based on near-universal orthologous gene groups, was applied to assess the transcriptome completeness. From a total of 1440 BUSCO groups searched, 77% were categorized as complete (41% as single-copy and 36% as duplicated), 10% as fragmented and 13% as missing BUSCOs. The gene expression comparison between red and white varieties of poinsettia showed a differential regulation of the flavonoid biosynthesis pathway only at particular stages of bract development. An initial impairment of the flavonoid pathway early in the color accumulation process for the white poinsettia variety was observed, but these differences were no longer present in the subsequent stages of bract development. Nonetheless, GSTF11 and UGT79B10 showed a lower expression in the last stage of bract development for the white variety and, therefore, are potential candidates for further studies on poinsettia coloration.ConclusionsIn summary, this transcriptome analysis provides a valuable foundation for further studies on poinsettia, such as plant breeding and genetics, and highlights crucial information on the molecular mechanism of color formation.

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Floral pigmentation pattern in Oriental hybrid lily ( Lilium spp.) cultivar ‘Dizzy’ is caused by transcriptional regulation of anthocyanin biosynthesis genes

Cited by 26 publications

References 61 publications

R2R3‐MYB genes control petal pigmentation patterning in Clarkia gracilis ssp. sonomensis (Onagraceae)

R2R3‐MYB genes control petal pigmentation patterning in Clarkia gracilis ssp. sonomensis (Onagraceae)

Cloning and Characterization of a Tryptophan–Aspartic Acid Repeat Gene Associated with the Regulation of Anthocyanin Biosynthesis in Oriental Hybrid Lily

Hybrid de novo transcriptome assembly of poinsettia (Euphorbia pulcherrima Willd. Ex Klotsch) bracts

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