Floral organ development goes live

Rambaud-Lavigne, Léa; Hay, Angela

doi:10.1093/jxb/eraa038

Cited by 7 publications

(5 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To understand how genes activity alters cellular growth, division, and differentiation in a controlled manner to produce a robust structural pattern, the methods described above must be coupled with approaches capable of capturing cell behavior with high spatiotemporal resolution. New powerful bioimaging techniques ( Prunet and Duncan, 2020 ; Rambaud-Lavigne and Hay, 2020 ) provide valuable tools to boost advances in the field.…”

Section: Understanding Pattern Formation On Plant Surfaces Using the Tools Of Modern Experimental Biologymentioning

confidence: 99%

Sculpting the surface: Structural patterning of plant epidermis

2021

View full text Add to dashboard Cite

Summary Plant epidermis are multifunctional surfaces that directly affect how plants interact with animals or microorganisms and influence their ability to harvest or protect from abiotic factors. To do this, plants rely on minuscule structures that confer remarkable properties to their outer layer. These microscopic features emerge from the hierarchical organization of epidermal cells with various shapes and dimensions combined with different elaborations of the cuticle, a protective film that covers plant surfaces. Understanding the properties and functions of those tridimensional elements as well as disentangling the mechanisms that control their formation and spatial distribution warrant a multidisciplinary approach. Here we show how interdisciplinary efforts of coupling modern tools of experimental biology, physics, and chemistry with advanced computational modeling and state-of-the art microscopy are yielding broad new insights into the seemingly arcane patterning processes that sculpt the outer layer of plants.

show abstract

Section: Understanding Pattern Formation On Plant Surfaces Using the Tools Of Modern Experimental Biologymentioning

confidence: 99%

Sculpting the surface: Structural patterning of plant epidermis

2021

View full text Add to dashboard Cite

show abstract

“…Time-lapse microscopy is the tool of choice for the spatiotemporal monitoring of organ growth at early developmental phase. State-of-the-art acquisition techniques allow following sample development only up to few days for animals (Berger et al, 2021 ; Park et al, 2015 ; Zattara et al, 2016 ), organoids (Hof et al, 2021 ), plant leaves (Kierzkowski et al, 2019 ; Serra and Perrot-Rechenmann, 2020 ) or floral organs (Fox et al, 2018 ; Rambaud-Lavigne and Hay, 2020 ; Ripoll et al, 2019 ). However, long-term observations are hampered by phototoxicity, photobleaching, tissue heating and manipulations that may significantly impact morphogenesis and increase lethality (Bell, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Model-based reconstruction of whole organ growth dynamics reveals invariant patterns in leaf morphogenesis

et al. 2023

View full text Add to dashboard Cite

Plant organ morphogenesis spans several orders of magnitude in time and space. Because of limitations in live-imaging, analysing whole organ growth from initiation to mature stages typically rely on static data sampled from different timepoints and individuals. We introduce a new model-based strategy for dating organs and for reconstructing morphogenetic trajectories over unlimited time windows based on static data. Using this approach, we show that Arabidopsis thaliana leaves are initiated at regular 1-day intervals. Despite contrasted adult morphologies, leaves of different ranks exhibited shared growth dynamics, with linear gradations of growth parameters according to leaf rank. At the sub-organ scale, successive serrations from same or different leaves also followed shared growth dynamics, suggesting that global and local leaf growth patterns are decoupled. Analysing mutants leaves with altered morphology highlighted the decorrelation between adult shapes and morphogenetic trajectories, thus stressing the benefits of our approach in identifying determinants and critical timepoints during organ morphogenesis.

show abstract

“…Understanding the complex interactions that link gene action to growth, and to different organ forms, requires the acquisition of technically challenging, quantitative data on cellular growth at high spatial and temporal resolution (3,48,59,93,122). One way to acquire such data is to use nondestructive live-imaging, which allows direct visualization and measurement of how form develops in different genetic backgrounds, while simultaneously monitoring the accompanying dynamic patterns of gene expression (12,126). However, although indispensable, such data are still inadequate to connect genetic changes with morphological divergence.…”

Section: From Genes To Models: Bridging the Gapmentioning

confidence: 99%

Leaf Shape Diversity: From Genetic Modules to Computational Models

Bhatia

Runions

Tsiantis

2021

Annu. Rev. Plant Biol.

View full text Add to dashboard Cite

Plant leaves display considerable variation in shape. Here, we introduce key aspects of leaf development, focusing on the morphogenetic basis of leaf shape diversity. We discuss the importance of the genetic control of the amount, duration, and direction of cellular growth for the emergence of leaf form. We highlight how the combined use of live imaging and computational frameworks can help conceptualize how regulated cellular growth is translated into different leaf shapes. In particular, we focus on the morphogenetic differences between simple and complex leaves and how carnivorous plants form three-dimensional insect traps. We discuss how evolution has shaped leaf diversity in the case of complex leaves, by tinkering with organ-wide growth and local growth repression, and in carnivorous plants, by modifying the relative growth of the lower and upper sides of the leaf primordium to create insect-digesting traps.

show abstract

Floral organ development goes live

Cited by 7 publications

References 38 publications

Sculpting the surface: Structural patterning of plant epidermis

Sculpting the surface: Structural patterning of plant epidermis

Model-based reconstruction of whole organ growth dynamics reveals invariant patterns in leaf morphogenesis

Leaf Shape Diversity: From Genetic Modules to Computational Models

Contact Info

Product

Resources

About