Floral humidity as a signal – not a cue – in a nocturnal pollination system

Dahake, Ajinkya; Jain, Piyush; Vogt, Caleb C.; Kandalaft, William; Stroock, Abraham D.; Raguso, Robert A.

doi:10.1101/2022.04.27.489805

Cited by 3 publications

(2 citation statements)

References 84 publications

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“…Based on homology to Drosophila IRs, MsexIR21a, MsexIR93a and MsexIR25a could have cool-temperature-sensing functions (Knecht et al, 2016;Ni et al, 2016). Alternatively, MsexIR93a and MsexIR25a might be involved in hygrosensation (Knecht et al, 2016) by neurons present in the styliform sensilla of the antenna (Dahake et al, 2022).…”

Section: Tissue Ors Irs Grsmentioning

confidence: 99%

Sex- and tissue-specific expression of chemosensory receptor genes in a hawkmoth

Tom

Llorca²,

Bucks³

et al. 2022

Front. Ecol. Evol.

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For the nocturnal hawkmoth Manduca sexta, olfactory and gustatory cues are essential for finding partners, food, and oviposition sites. Three chemosensory receptor families, odorant receptors (ORs), ionotropic receptors (IRs), and gustatory receptors (GRs) are involved in the detection of these stimuli. While many chemosensory receptor genes have been identified, knowledge of their expression profile in potentially chemoreceptive organs is incomplete. Here, we studied the expression patterns of chemosensory receptors in different tissues including the antennae, labial palps, proboscis, legs, wings and ovipositor. We compared the receptors’ expression in female and male moths both before and after mating by using the NanoString platform. This tool allowed us to measure expression levels of chemosensory receptor genes in a single reaction using probes designed against 71 OR, 29 IR and 49 GR transcripts. In all tissues investigated, we detected expression of genes from all three receptor families. The highest number of receptors was detected in the antennae (92), followed by the ovipositor (59), while the least number was detected in the hindlegs (21). The highest number of OR genes were expressed in the antennae (63), of which 24 were specific to this main olfactory organ. The highest number of IRs were also expressed in the antennae (16), followed by the ovipositor (15). Likewise, antennae and ovipositor expressed the highest number of GRs (13 and 14). Expression of the OR co-receptor MsexORCo, presumably a prerequisite for OR function, was found in the antennae, labial palps, forelegs and ovipositor. IR co-receptors MsexIR25a and MsexIR76b were expressed across all tested tissues, while expression of the IR co-receptor MsexIR8a was restricted to antennae and ovipositor. Comparing the levels of all 149 transcripts across the nine tested tissues allowed us to identify sex-biased gene expression in the antennae and the legs, two appendages that are also morphologically different between the sexes. However, none of the chemosensory receptors was differentially expressed based on the moths’ mating state. The observed gene expression patterns form a strong base for the functional characterization of chemosensory receptors and the understanding of olfaction and gustation at the molecular level in M. sexta.

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Section: Tissue Ors Irs Grsmentioning

confidence: 99%

Sex- and tissue-specific expression of chemosensory receptor genes in a hawkmoth

Tom

Llorca²,

Bucks³

et al. 2022

Front. Ecol. Evol.

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“…Automated tracking studies of insects are often carried out in controlled environments, which is feasible when the behavior of interest is robust to laboratory conditions. For example, digital tracking has been used to characterize the foraging behavior of hawkmoths Manduca sexta [36][37][38], and to characterize the wing kinematics of flies and honey bees as well as honey bee wing fanning behavior [39][40][41]. Complex social behaviors are less robust to experimental laboratory conditions, requiring field observations to draw reliable conclusions.…”

Section: Discussionmentioning

confidence: 99%

Postural analysis reveals persistent vigilance in paper wasps after conspecific challenge

Legan

Vogt

Sheehan

2022

Preprint

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Vigilant animals detect and respond to threats in the environment, often changing posture and movement patterns. In social animals vigilance is modulated not only by predators but also by threatening conspecifics. Precisely how social interactions alter vigilance behavior over time is not well understood. We report persistent effects of a simulated social challenge on the vigilance behavior of wild northern paper wasp foundresses, Polistes fuscatus. During the founding phase of the colony cycle conspecific wasps may usurp nests from the resident foundress, representing a severe threat. Using postural tracking, we found that after simulated intrusions wasps displayed increased vigilance during the minutes after the threat was removed. Sustained vigilance elicited after social threat manifested as increased movement, greater bilateral wing extension, and reduced antennal separation. However, no postural changes were observed after a control stimulus presentation. By rapidly adjusting individual vigilance behavior after fending off a conspecific intruder, paper wasp foundresses invest in surveillance of potential social threats, even when such threats are no longer immediately present. The prolonged state of vigilance observed here is relevant to plasticity of recognition processes as a result of conspecific threats.

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Simple Behavioral Analysis (SimBA) as a platform for explainable machine learning in behavioral neuroscience

Goodwin,

Choong,

Hwang

et al. 2024

Nat Neurosci

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Floral humidity as a signal – not a cue – in a nocturnal pollination system

Cited by 3 publications

References 84 publications

Sex- and tissue-specific expression of chemosensory receptor genes in a hawkmoth

Sex- and tissue-specific expression of chemosensory receptor genes in a hawkmoth

Postural analysis reveals persistent vigilance in paper wasps after conspecific challenge

Simple Behavioral Analysis (SimBA) as a platform for explainable machine learning in behavioral neuroscience

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