Floral Extracts of Allamanda Blanchetii and Allamanda Cathartica Are Comparatively Higher Resource of Anti-Oxidants and Polysaccharides Than Leaf and Stem Extracts

Abstract

Objective: The present study undertakes a comparative analysis of the level of secondary metabolites present in the leaf, flower and stem of the two ornamental plants, Allamanda blanchetii and Allamanda cathartica. Methods:The two plant species, Allamanda blanchetii and Allamanda cathartica were collected, washed, shade dried in room temperature and powered in mechanical grinder. Phytochemicals were extracted from the power with methanol and double distilled water. The estimation of flavonoids, polyphenols, polysaccharide were done by standard methods and the anti-oxidant activity was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) discoloration assay.Results: Our study reveals that the flower of both species contain highest amount of secondary metabolites in crude methanolic and aqueous extracts. In case of leaf, the methanolic extracts contain higher amount of polyphenol, flavonoid and anti-oxidant property in comparison to aqueous extracts, where as the aqueous extract contain higher amount of polysaccharide content than its counterpart. In stem, crude organic extract has higher amount of polyphenol and flavonoid and the aqueous extract has higher amount of polysaccharide and anti-oxidant property. Conclusion:The flower of Allamanda cathartica and Allamanda blanchetii has higher amount of flavonoids, polyphenols, polysaccharide and the floral extracts display comparatively higher anti-oxidant property.

Phytochemical and Biological Study of Trophic Interaction between Pseudosphinx Tetrio L. Larvae and Allamanda Cathartica L.

“…The plant is native to Brazil [ 24 ] and its various parts such as leaves, flowers, roots and stems are used in traditional medicine [ 25 ] for the treatment of jaundice [ 26 ], and have various pharmacological activities such as wound healing [ 26 ], antioxidant [ 27 , 28 , 29 , 30 ], antimicrobial [ 30 , 31 , 32 ], antifungal [ 33 ], antimalarial [ 34 ], anti-inflammatory [ 35 ]; anticancer [ 20 , 36 , 37 ] and gastrointestinal effects [ 38 ]. A dichloromethane (CH 2 Cl 2 ) extract of the whole plant tested on two pathogenic dermatophytes, Trichophyton rubrum and Microsporum gypseum , showed moderate activity at a concentration of 50 μg/disk, but very strong activity at a concentration of 200 μg/disk, suggesting that A. cathartica may possess antidermatophyte constituents that could be useful in the treatment of ringworm [ 39 , 40 ].…”

“…A total of 151 compounds were distributed as follows: 3 hydrocarbons in flowers; 7 alcohol compounds, 9 esters, 1 ether, 6 aldehydes, and 1 ketone in flowers, leaves, and stems extracts; 37 fatty acids and phospholipids; 43 volatile compounds mostly in flowers and leaves; 5 phenolic compounds and 6 flavonoids in the flowers and stems; 2 alkaloids in stems; 11 steroids and terpenes in leaves, stems and flowers; and 14 lactones in roots, stems, leaves, flowers and bark; 6 carbohydrates in leaves, stems, and nectar. Of the compounds identified, leaves contain: ursolic acid, β-amyrin; β-sitosterol, sesquiterpenes, plumericin, plumieride, long chain esters, flavonoids, polyphenols, allamandin, alkaloids, saponins and carbohydrates; stem and bark contain: ursolic acid, β-amyrin, β-sitosterol, triterpenoids, glucosides, alkaloids, flavonoids and polyphenols; flowers contain: quercetin, quercitrin, kaempferol, hesperetin, flavonoids, polyphenols and polysaccharides; and roots contain: lactones, allamandin, allamandicin, allamdin, plumieride iridoids, triterpenoids, alkaloids and several glucosides [ 36 , 38 , 43 ] (cited by [ 44 ]); [ 19 , 21 , 27 , 42 ]. Like A. cathartica , various parts of P. rubra are widely used in traditional medicine [ 45 ].…”