Floral classification of honey using liquid chromatography–diode array detection–tandem mass spectrometry and chemometric analysis

Zhou, Jinhui; Yao, Lun; Li, Yi; Chen, Lanzhen; Wu, Liming; Zhao, Jing

doi:10.1016/j.foodchem.2013.08.117

Cited by 81 publications

(45 citation statements)

References 19 publications

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“…In numerous studies, it has been proposed that phenolic compounds can be very useful biomarkers for discrimination of honeys according to their floral source . This part of the study aimed to identify the phenolic acid and flavonoid constituents which could be considered as the main honey floral origin markers of Romanian honeys.…”

Section: Resultscontrasting

confidence: 99%

“…Association of honey phenolic profile with pollen analysis and other physico‐chemical analysis is practiced . Ellagic acid and pinocembrin were identified as floral markers for polyfloral Belgian honeys, while kaempferol, morin and ferulic acid were used as floral markers to distinguish Chinese rape honey . Bertoncelj el al .…”

Section: Introductionmentioning

confidence: 99%

“…The analytical procedures used to determine polyphenols in honey involve their extraction from the honey matrix, and their chromatographic separation followed by quantification . Numerous studies of honey phenolic acids and flavonoids have been focused on the extraction of the phenolic compounds from honey using the Amberlite XAD‐2 resin, solid‐phase extraction (SPE) procedures with commercial cartridges (Bond Elut octadecyl C18, Sep‐Pak RP C18, Oasis HLB and Strata‐X) or liquid–liquid extraction methods prior to their identification and quantification. Liquid chromatography (LC) is considered to be the most useful separation technique for the analysis of polyphenols in honey, including high‐performance liquid chromatography diode array detector (HPLC‐DAD) and HPLC‐mass spectrometry (MS) for quantitative measurements …”

Section: Introductionmentioning

confidence: 99%

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Phenolic compounds profile and biochemical properties of honeys in relationship to the honey floral sources

Ciucure

Geană

2019

Phytochemical Analysis

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Introduction Honey has been considered to have therapeutic properties since ancient times and among the factors responsible for such activity are phenolic compounds including phenolic acids and flavonoids from different natural sources. Objective This study investigated the phenolic compounds profile and bioactive properties of different honey types from Romanian flora in order to develop reliable tools for honey floral origin, thus contributing to the honey traceability in the European Union context. Material and methods Thirty‐three honey samples were examined, including unifloral (acacia and rape), polyfloral, honeydew honeys and mixture honeys. Phenolic acids and flavonoids were isolated from the water soluble honey matrix using a solid‐phase extraction (SPE) method and analysed by ultra‐high‐performance liquid chromatography diode array detector electrospray ionisation mass spectrometry (UHPLC‐DAD‐ESI/MS). Honey bioactive properties were measured in honey dissolved in 80% ethanol using UV‐visible spectrophotometric methods. Multivariate statistical tools (principal component analysis and hierarchical clustering analysis) were used for honey classification. Results The results of this study confirm that honey samples had similar, but quantitatively different, phenolic acids and flavonoids profiles and bioactive properties, related with honey floral source. Coloured honeys, such as honeydew honey, show high phenolic composition and bioactive properties and implicitly a high therapeutic potential compared with the other floral honeys. Conclusion Distinctive clusters obtained by principal component analysis enabled us to consider that honeydew and polyfloral honeys could be distinguished from acacia and rape honey with the analytical methods developed. Based on this study, the methods might be promising tools for honey traceability, which needs to be explored on a larger set of samples with different regional floral origins in future studies.

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Section: Resultscontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phenolic compounds profile and biochemical properties of honeys in relationship to the honey floral sources

Ciucure

Geană

2019

Phytochemical Analysis

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“…Grouping of honey with similar floral sources could be predicted via pattern of recognition through multivariance analysis technique. Moreover, classification of honey in association with floral source using chromatographic profiling combined with chemometrics analysis has been recently introduced (Zhou et al ., ; Esteki et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Impact of botanical source and processing conditions on physicochemical properties and antioxidant activity of honey in the northern part of Thailand

Hempattarasuwan

Settachaimongkon

Duangmal

2019

Int J of Food Sci Tech

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Summary Phenolics, melissopalynological analysis, and selected physicochemical characteristics could differentiate floral honey origin. Botanical sources (longan, multifloral and Manuka) had more pronounced effect on honey properties than processing conditions. The conductivity was related to ash content and colour of honey. Total sugar, fructose and glucose content in all samples were not significantly different. Melissopalynological analysis was used to verify the botanical source of honey samples. Predominant pollen was identified. Results characterising antioxidant properties (DPPH radicals, hydroxyl radicals, and carbon‐centred radicals scavenging activity) were confirmed via both Electron Spinning Resonance and oxygen radical absorbance capacity (ORAC). They showed that only hydroxyl radicals scavenging activity and ORAC were significantly different. Type and amount of phenolics in honey samples identified using HPLC could differentiate botanical source. Gallic acid and kaempferol were potential marker phenolics for longan honey. Multivariate statistical analysis allowed discrimination of honey from different botanical sources based on their phenolic profiles.

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“…Therefore, the determination of pesticide residues in honey is a challenge, especially because of the low concentration of analytes and large amounts of interfering substances which can be coextracted with analytes, and in most cases, adversely affect analysis results (Chudzinska et al 2012;Kujawski and Namiesnik 2008). Honey contains a large amount of sugars, proteins, amino acids, vitamins, antioxidants, and pigments (Zhou et al 2014;Ferreira et al 2009). The amount of these compounds largely depends upon the variety of the honey.…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatographic MS/MS Analysis of a Large Group of Insecticides in Honey by Modified QuEChERS

2018

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The analytical tool for analysis of pesticide residues in honey must still be improved. Therefore, a sensitive, selective, rapid, and reliable analytical method for the analysis of pesticide residue belonging to 29 different classes at very low concentration levels was developed. Over 130 insecticides were extracted from the honey matrix using different amounts of sample, concentrations of the extraction solvent, and cleanup sorbents. Acceptable extraction recoveries accounting for matrix effect were obtained using the cleanup sorbent combination chitosan/aluminum oxide/C8 (200/200/200 mg) and 1% FA in ACN. Determinations were made by liquid chromatography-tandem mass spectrometry (LC-MS/MS). For most compounds, the recoveries range from 70 to 120%, with relative standard deviations < 20%. Linear correlation coefficients (r2) were higher than 0.99 at concentration levels of 0.001-0.100 mg kg−1. A positive matrix effect was observed for up to 95% of organophosphates and 43% of pyrethroids and a negative effect for 37% of neonicotinoids. This work showed that the presented method is convenient and reliable for quick monitoring of insecticides in honey samples.

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Floral classification of honey using liquid chromatography–diode array detection–tandem mass spectrometry and chemometric analysis

Cited by 81 publications

References 19 publications

Phenolic compounds profile and biochemical properties of honeys in relationship to the honey floral sources

Phenolic compounds profile and biochemical properties of honeys in relationship to the honey floral sources

Impact of botanical source and processing conditions on physicochemical properties and antioxidant activity of honey in the northern part of Thailand

Liquid Chromatographic MS/MS Analysis of a Large Group of Insecticides in Honey by Modified QuEChERS

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