Flora bakteryjna występująca na powierzchni i w miąższu migdałków w przewlekłym zapaleniu migdałków podniebiennych

Syryło, Agnieszka; Wojdas, Andrzej; Jurkiewicz, Dariusz

doi:10.1016/s0030-6657(07)70493-6

Cited by 4 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Streptococcus pyogenes strains were co-isolated in four patients with RT, and none were isolated in patients with a PTA. Other studies have also reported a low isolation rate (1.7–5%) of streptococci in patients with RT; streptococci have been found to be less prevalent in the tonsillar core (1.7%) [ 29 , 31 ] than on the tonsillar surface. S. pyogenes in the RT pathogenesis has most likely been overrated or, alternatively, decreased in recent years [ 2 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Microorganism Colonization, Biofilm Production, and Antibacterial Susceptibility in Recurrent Tonsillitis and Peritonsillar Abscess Patients

Klagisa

Racenis²,

Broks³

et al. 2022

IJMS

View full text Add to dashboard Cite

Background: Despite the widespread use of antibiotics to treat infected tonsils, episodes of tonsillitis tend to recur and turn into recurrent tonsillitis (RT) or are complicated by peritonsillar abscesses (PTAs). The treatment of RT and PTAs remains surgical, and tonsillectomies are still relevant. Materials and methods: In a prospective, controlled study, we analyzed the bacteria of the tonsillar crypts of 99 patients with RT and 29 patients with a PTA. We performed the biofilm formation and antibacterial susceptibility testing of strains isolated from study patients. We compared the results obtained between patient groups with the aim to identify any differences that may contribute to ongoing symptoms of RT or that may play a role in developing PTAs. Results: The greatest diversity of microorganisms was found in patients with RT. Gram-positive bacteria were predominant in both groups. Candida species were predominant in patients with a PTA (48.3% of cases). Irrespective of patient group, the most commonly isolated pathogenic bacterium was S. aureus (in 33.3% of RT cases and in 24.14% of PTA cases). The most prevalent Gram-negative bacterium was K. pneumoniae (in 10.1% of RT cases and in 13.4% of PTA cases). At least one biofilm-producing strain was found in 37.4% of RT cases and in 27.6% of PTA cases. Moderate or strong biofilm producers were detected in 16 out of 37 cases of RT and in 2 out of 8 PTA cases. There was a statistically significant association found between the presence of Gram-positive bacteria and a biofilm-formation phenotype in the RT group and PTA group (Pearson χ2 test, p < 0.001). S. aureus and K. pneumoniae strains were sensitive to commonly used antibiotics. One S. aureus isolate was identified as MRSA. Conclusions: S. aureus is the most common pathogen isolated from patients with RT, and Candida spp. are the most common pathogens isolated from patients with a PTA. S. aureus isolates are susceptible to most antibiotics. Patients with RT more commonly have biofilm-producing strains, but patients with a PTA more commonly have biofilm non-producer strains. K. pneumoniae does not play a major role in biofilm production.

show abstract

Section: Discussionmentioning

confidence: 99%

Analysis of Microorganism Colonization, Biofilm Production, and Antibacterial Susceptibility in Recurrent Tonsillitis and Peritonsillar Abscess Patients

Klagisa

Racenis²,

Broks³

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Bacteremia in the patients who have experienced tonsillectomy due to chronic tonsillitis may lead to intense outcomes, especially in those who are faced with the risk of cardiovascular infection [6, 7]. Some studies have shown that the bacterial pathogens related to tonsillitis inhabited the surface and depth of the tonsil tissue [8]. Moreover, a large number of studies have presented similarities between the types of isolated microorganisms from the surface and depth of tonsil, but the degree of likeness has been very different in these studies [6, 9–11].…”

Section: Introductionmentioning

confidence: 99%

Posttonsillectomy Bacteremia and Comparison of Tonsillar Surface and Deep Culture

Shishegar

Ashraf

2014

Advances in Preventive Medicine

View full text Add to dashboard Cite

Objective. This study aimed to identify the microorganisms of surface and depth of tonsils and whether these microorganisms bring the menace of bacteremia during tonsillectomy in the children under surgery. Materials and Methods. The culture specimens were taken from surface and depth of tonsil from the patients suffering from chronic tonsillitis at the time of operation. Also, 10 mL venous blood samples were taken 5 minutes before and after the operation for microbiological study. Results. According to the results, 112 (76.1%) and 117 (79.6%) cultures from surface and depth of tonsils represented multiple microorganisms, respectively. Besides, staphylococci coagulase positive was the most common organism in both surface and depth of tonsils. None of the preoperation blood cultures were positive, while 3 postoperation blood cultures (2.1%) were positive. Staphylococci coagulase negative and alpha hemolytic streptococcus were detected in 2 cases (1.4%) and 1 case (0.7%), respectively. Conclusion. In the present study, the two cultured sites were almost similar regarding the types of isolated microorganisms. Our results suggested that bacteremia might occur after tonsillectomy. Therefore, to avoid the possible dramatic outcomes after tonsillectomy, pre- and postoperation attendances are essential.

show abstract

“…S. pyogenes strains were isolated with other microorganisms in 4 patients with RT, but not in any patient with PTA. Also, in other studies, a low (1.7-5 %) frequency of streptococci isolation in patients with RT has been found, it has been proven that they are more often found on the surface of the palatine tonsils than in the tonsils(Syryło et al, 2007;Alasil et al, 2013). The role of S. pyogenes in the pathogenesis of RT has been overestimated or has been decreased over the years(Lindroos, 2000; Zautner et al, 2010; Kostić et al, 2022).…”

mentioning

confidence: 97%

Role of Biofilms in Pathogenesis and Clinical Course of Recurrent Tonsillitis and Peritonsillar Abscess. Summary of the Doctoral Thesis

Vīksne (Klagiša)

View full text Add to dashboard Cite

After nasopharyngitis and otitis media tonsillitis is the 3rd most common infection that requires patients to see an otorhinolaryngologist. When episodes of tonsillitis recur, acute tonsillitis diagnosis turns into recurrent tonsillitis (RT). Exacerbations of RT are treated with antibiotics. Currently, the frequency of exacerbations in the last 3 years is used as the main determining factor in the selection of patients for elective tonsillectomy. If there are 7 episodes of tonsillitis in a year, or 5 episodes of tonsillitis in a year for 2 consecutive years, or 3 episodes of tonsillitis in a year for 3 consecutive years, tonsillectomy is recommended. The etiopathogenesis of RT is not clear and is being actively studied. The reason for ineffective antibacterial therapy is explained by specific resistance mechanisms of bacteria, formation of biofilms, insufficient concentration of antibiotics in tonsillar tissue. RT reduces patients` quality of life and is a medical and a financial burden. During exacerbation of RT a peritonsillar abscess (PTA) as a complication may develop. Therefore, the aim of the study was to identify the etiological agents of RT and PTA in tonsil crypts and parenchyma samples, to determine antibacterial resistance and biofilm formation capacity of pathogenic bacteria, and to evaluate their importance in the pathogenesis and clinical course of RT and PTA. The study included several parts: 1st part was dedicated for evaluating RT and PTA patients undergoing tonsillectomy. The RT group consisted of 99 patients who underwent planned tonsillectomy due to RT despite appropriate antibacterial therapy. The PTA patient group consisted of 29 patients who underwent acute tonsillectomy for drainage of peritonsillar abscess. The objective of 2nd part was to evaluate the most accurate method of obtaining samples from tonsillar crypts. The study group of 25 patients with RT was created. In this part, tonsillar crypt samples were obtained in 2 ways and the results were compared. The objective of 3rd part was to evaluate the microbiological results of tonsillar crypt sample of healthy individuals. A group of healthy individuals without tonsillar pathology consisted of 91 participants – students of Medical Faculty of Rīga Stradiņš University. In 4th part a bacteriological evaluation of post-tonsillectomy patients was performed. The group consisted of 16 patients who previously had RT and had a tonsillectomy performed a year ago. These patients were subjected to clinical and bacteriological evaluation. During each part tonsillar crypt samples were taken for bacteriological analysis, antibacterial sensitivity and biofilm production capacity of isolated strains was assessed. The examinations were carried out in the laboratory of the Biology and Microbiology Department of Rīga Stradiņš University and the Laboratory of Bacteriology of the Pauls Stradiņš Clinical University Hospital. During the study a biopsy needle was developed and patented for obtaining an optimal sample of tonsillar crypts. The study found that RT patients, compared to PTA patients and healthy individuals, had a higher diversity of microorganisms in the tonsillar crypts, the predominant pathogen being S. aureus. Biofilm forming strains were detected in RT, PTA patients, but no significant antibacterial resistance was detected and no relationship between the biofilm producing phenotype and antibacterial sensitivity was observed. Clinically relevant pathogens (S. aureus, K. pneumoniae, P. aeruginosa) and susceptible biofilm producing strains were isolated from tonsillar crypts of healthy individuals without tonsillar pathology. The tonsillar crypts of healthy individuals are an important reservoir of pathogenic bacteria and biofilm producing strains. The results of our study confirm that tonsillectomy is effective in preventing bacterial colonization. The thesis is presented as a unifying material of peer-review publications, it summarizes the results of the research. The obtained results on RT and oral microbiota are of international importance and contain recommendations for use in clinical practice. 3.2 Clinical Medicine; Sub-Sector – Otorhinolaryngology. Keywords: recurrent tonsillitis, peritonsillar abscess, biofilms, antibacterial susceptibility.

show abstract

Flora bakteryjna występująca na powierzchni i w miąższu migdałków w przewlekłym zapaleniu migdałków podniebiennych

Cited by 4 publications

References 7 publications

Analysis of Microorganism Colonization, Biofilm Production, and Antibacterial Susceptibility in Recurrent Tonsillitis and Peritonsillar Abscess Patients

Analysis of Microorganism Colonization, Biofilm Production, and Antibacterial Susceptibility in Recurrent Tonsillitis and Peritonsillar Abscess Patients

Posttonsillectomy Bacteremia and Comparison of Tonsillar Surface and Deep Culture

Role of Biofilms in Pathogenesis and Clinical Course of Recurrent Tonsillitis and Peritonsillar Abscess. Summary of the Doctoral Thesis

Contact Info

Product

Resources

About