Flooding Stress-Induced Glycine-Rich RNA-Binding Protein from Nicotiana tabacum

Lee, Mi‐Ok; Kim, Keun Pill; Kim, Byung-gee; Hahn, Jinyong; Hong, Choo Bong

doi:10.1007/s10059-009-0004-4

Cited by 33 publications

(17 citation statements)

References 52 publications

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“…Here, we show that MpGR-RBP1 gene expression is strongly up-regulated upon drought stress. This observation is consistent with the results shown by Lee et al (2009), where tobacco NtGRP1 is also upregulated by drought, albeit to a much lower degree compared to flooding stress. On the contrary, the transcript level of GR-RBP4 in Arabidopsis decreases slightly with dehydration stress (Kwak et al 2005).…”

Section: Discussionsupporting

confidence: 94%

“…Although a few such GR-RBPs involved in abiotic stress responses have been identified in Arabidopsis, rice, and tobacco (Sahi et al 2007;JS Kim et al 2008;Lee et al 2009), little information has been reported for GR-RBPs in Malus plants against water stress. Therefore, it is necessary to investigate this relationship because the development of drought-resistant rootstocks through the modification of stress-related genes can lead to enhanced fruit crop productivity.…”

Section: Discussionmentioning

confidence: 98%

“…RBPs contain one or more RRMs at the N-terminus and a variety of auxiliary motifs at the C-terminus, such as glycine-rich, arginine-rich, SR-repeat, RD-repeat, and acidic domains (JS Kim et al 2008;Lee et al 2009). Plant GR-RBPs belong to the family of RNA-binding proteins that has in common a protein domain that is necessary and sufficient for RNA binding.…”

Section: Discussionmentioning

confidence: 99%

“…Since the first gene encoding GR-RBP was identified in immature maize embryos (Gόmez et al 1988), other such cDNAs have been isolated from monocots, dicots, and gymnosperms, including WhGRP-1 in wheat (Guiltinan and Niu 1996), ngRBP in tobacco (Naqvi et al 1998), SCRGP-1 in wild potato (Baudo et al 1999), PgRNP in white spruce (Richard et al 1999), sbGR-RNP in broomcorn (Aneeta et al 2002), LpGRP1 in perennial ryegrass (Shinozuka et al 2006), and NtGRP1 in tobacco (Lee et al 2009). All of the proteins encoded by these genes share similar structural features, including an RRM at the N-terminal half and glycine-rich domains at the C-terminal half.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of a Novel Drought Responsive Gene Encoding a Glycine-rich RNA-binding Protein in Malus prunifolia (Willd.) Borkh.

et al. 2010

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Drought is one of the most severe environmental factors that impair plant growth and agricultural production. To investigate how Malus prunifolia, an excellent apple rootstock with strong drought tolerance, adapts to stress and to identify genes for improving this important trait, we constructed a suppression subtractive hybridization cDNA library from seedling leaves under water stress. The cDNA for a drought-inducible glycine-rich RNA-binding protein (GR-RBP) was isolated, and the gene was characterized for its role in the response of Malus seedlings to drought stress. cDNA clone MpGR-RBP1 has 781 nucleotides, with an open reading frame of 516 nucleotides. The deduced 171 amino acids contain an amino-terminal RNA recognition motif and a carboxyl-terminal glycine-rich domain, with structural similarity to a class of stress-induced GR-RBP proteins found in other plants. Phylogenetic analysis confirmed that the MpGR-RBP1 protein belongs to the plant GR-RBP family, members of which play important roles in posttranscriptional regulation of gene expression under various stress conditions. The expression profile of MpGR-RBP1 transcripts was detected by quantitative realtime polymerase chain reaction (PCR) and semiquantitative reverse transcriptase PCR. MpGR-RBP1 was expressed in both roots and leaves, with expression being higher in the latter. This is the first report of this class of protein in Malus plants, and the putative role of MpGR-RBP1 is discussed.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of a Novel Drought Responsive Gene Encoding a Glycine-rich RNA-binding Protein in Malus prunifolia (Willd.) Borkh.

et al. 2010

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“…GRA influences plant growth and development, usually in response to environmental or oxidative stress [49,50]. DEAD box RNA helicases function as molecular motors to rearrange RNA secondary structure or act as mediators of RNA-protein association/disassociation.…”

Section: 3mentioning

confidence: 99%

Comparative proteomic analysis reveals the mechanisms governing cotton fiber differentiation and initiation

Liu

Han

Zhang

et al. 2012

Journal of Proteomics

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Protein expression changes during cotton fiber elongation in response to drought stress and recovery

Meng

Yang

et al. 2014

Proteomics

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An investigation to better understand the molecular mechanism of cotton (Gossypium hirsutum L.) fiber elongation in response to drought stress and recovery was conducted using a comparative proteomics analysis. Cotton plants (cv. NuCOTN 33B) were subjected to water deprivation for 10 days followed by a recovery period (with watering) of 5 days. The temporal changes in total proteins in cotton fibers were examined using 2DE. The results revealed that 163 proteins are significantly drought responsive. MS analysis led to the identification of 132 differentially expressed proteins that include some known as well as some novel drought-responsive proteins. These drought responsive fiber proteins in NuCOTN 33B are associated with a variety of cellular functions, i.e. signal transduction, protein processing, redox homeostasis, cell wall modification, metabolisms of carbon, energy, lipid, lignin, and flavonoid. The results suggest that the enhancement of the perception of drought stress, a new balance of the metabolism of the biosynthesis of cell wall components and cytoskeleton homeostasis plays an important role in the response of cotton fibers to drought stress. Overall, the current study provides an overview of the molecular mechanism of drought response in cotton fiber cells.

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Flooding Stress-Induced Glycine-Rich RNA-Binding Protein from Nicotiana tabacum

Cited by 33 publications

References 52 publications

Isolation and Characterization of a Novel Drought Responsive Gene Encoding a Glycine-rich RNA-binding Protein in Malus prunifolia (Willd.) Borkh.

Isolation and Characterization of a Novel Drought Responsive Gene Encoding a Glycine-rich RNA-binding Protein in Malus prunifolia (Willd.) Borkh.

Comparative proteomic analysis reveals the mechanisms governing cotton fiber differentiation and initiation

Protein expression changes during cotton fiber elongation in response to drought stress and recovery

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