Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River

Aborgiba, Mustafa; Kostić, Jovana; Kolarević, Stoimir; Kračun-Kolarević, Margareta; El-Bahi, S.M.; Knežević-Vukčević, Jelena; Lenhardt, Mirjana; Paunović, Momír; Gačić, Zoran; Vuković-Gačić, Branka

doi:10.1016/j.scitotenv.2015.03.120

Cited by 27 publications

(9 citation statements)

References 61 publications

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“…In this study we have performed comprehensive genotoxicological survey by applying the battery of assays in prokaryotes and aquatic eukaryotes. When constructing the battery of bioassays we were focused on types of assays which have already been employed earlier in ecogenotoxicological studies of the Sava River [ 8 – 14 , 27 ]. Also, we have used experience gained during the Joint Danube Survey 3 –JDS3 [ 22 , 28 , 29 , 30 ] for updating the assay list as usage of the same methodology would enable comparison of water quality of the Sava and Danube in further research.…”

Section: Discussionmentioning

confidence: 99%

“…Previously we have demonstrated that the area is under pressure of genotoxic pollution which is reflected in animals belonging to different trophic levels. [ 12 , 14 ]. At this site, we have detected a significant increase of DNA damage measured by TI%, and increased levels of oxidative stress in comparison with the reference site, which is in compliance with the data on genotoxicity previously obtained in bream species [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

“…Ames test on Salmonella typhimurium ). In our previous research we were focused on the lower stretch of the Sava River which comprises the urban area of Belgrade city [ 12 – 14 ]. Hereby the research was performed on freshwater mussels and freshwater fish (comet assay).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Genotoxic Pressure along the Sava River

et al. 2016

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In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg—modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg—modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Evaluation of Genotoxic Pressure along the Sava River

et al. 2016

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“…In freshwater environments, the zebra mussel (Dreissena polymorpha) is the most common bivalve for genotoxicity assessments using the comet assay [134][135][136][137][138][139][140][141]. Several other freshwater species are also used, such as the quagga mussel (Dreissena bugensis) [137], painter's mussel (Unio pictorum) [142][143][144][145][146], swollen river mussel (Unio tumidus) [142,143,145,147,148], freshwater mussel (Unio tigridis) [149], golden mussel (Limnoperna fortunei) [150,151], Chinese pond mussel (Sinanodonta woodiana) [143,152], Asian clam A c c e p t e d M a n u s c r i p t 10 (Corbicula fluminea) [153][154][155], Lamellidens marginalis [156] and paper pondshell (Utterbackia imbecillis) [157].…”

Section: Bivalvesmentioning

confidence: 99%

“…The DNAdamaging effects of both ionising [212,228] and non-ionising radiation [229] were also studied, indicating that both types of radiation are able to induce DNA damage and that the comet assay is a sensitive and rapid method for the detection of radiation-induced genotoxicity. There are many other studies on terrestrial as well as aquatic oligochaete species such as Eisenia hortensis [230,231], several species of lumbricids (Lumbricus terrestris, Lumbricus rubellus, Lumbricus castaneous) [232][233][234], Amynthas diffringens [235], Amynthas gracilis [236], Aporrectodea caliginosa [235,237], Branchiura sowerbyi [148], Dendrodrilus rubidus [232,235], Dichogaster curgensis [238][239][240], Limnodrilus udekemianus Claparede [241], Metaphire posthuma [242], Microchaetus benhami [235], Enchytraeus crypticus [243] and Pheretima peguana [244]. Since some studies also showed differences in sensitivity between the tested species in response towards genotoxicants [197,232,235] special attention should be given when choosing appropriate species for biomonitoring studies in order to reduce both false positive and false negative results.…”

Section: Oligochaetesmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay, also called single cell gel electrophoresis, is a sensitive, rapid and low-cost technique for quantifying and analysing DNA damage and repair at the level of individual cells. The assay itself can be applied on virtually any cell type derived from different organs and tissues of eukaryotic organisms. Although it is mainly used on human cells, the assay has applications also in the evaluation of DNA damage in yeast, plant and animal cells. Therefore, the purpose of this review is to give an extensive overview on the usage of the comet assay in animal models from invertebrates to vertebrates, covering both terrestrial and water biota. The comet assay is used in a variety of invertebrate species since they are regarded as interesting subjects in ecotoxicological research due to their significance in ecosystems. Hence, the first part of the review (Part 1) will discuss the application of the comet assay in invertebrates covering protozoans, platyhelminthes, planarians, cnidarians, molluscs, annelids, arthropods and echinoderms. Besides a large number of animal species, the assay is also performed on a variety of cells, which includes haemolymph, gills, digestive gland, sperm and embryo cells. The mentioned cells have been used for the evaluation of a broad spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of invertebrate models and their role from an ecotoxicological point of view will also be discussed as well as the comparison of the use of the comet assay in invertebrate and human models. Since the comet assay is still developing, its increasing potential in assessing DNA damage in animal models is crucial especially in the field of ecotoxicology and biomonitoring at the level of different species, not only humans.

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Contamination and genotoxicity biomarker responses in bivalve mussels from the major Lithuanian rivers

Rybakovas

Arbačiauskas

Markovskienė

et al. 2019

Environ and Mol Mutagen

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European inland waters are under continuous threat of anthropogenic pollution. Determination of background level of biomarker response and subsequent classification of the impact increases the applicability of results. In the current study, we evaluate the range of chemical contamination by measuring the concentrations of metals, polycyclic aromatic hydrocarbons and polychlorinated biphenyls, and the levels of environmental genotoxicity by using the micronuclei and nuclear bud tests in bivalve mussels of the major Lithuanian rivers. Second, we aimed to evaluate the association between chemical contamination and genotoxicity biomarker responses. Finally, we set to determine the background level of genotoxic effects. Such value (summed frequency of MN and NB) was assessed—6‰. On that basis, we develop a scale of potential genotoxic impact and perform ranging sites into five categories. The results clearly indicate the existence of significant differences in the levels of chemical pollution and genotoxicity in different sites. Increased levels of studied parameters were assessed at the areas affected by municipal and industrial effluents, road runoff, combustion products, and in the area contaminated by accidental spillage. On the other hand, downstream decrease of contamination level, presumably associated with biological degradation and photochemical oxidation, were also observed. Genotoxicity parameters were associated with PAH and metal concentrations measured in mussel tissues as well as in sediments. Results also indicate that in situ genotoxicity assessment performed in the areas affected by long‐term contamination of municipal origin might be not sufficiently precise. Study highlights the necessity to combine genotoxicity assessment with chemical analysis. Environ. Mol. Mutagen. 61:338–354, 2020. © 2019 Wiley Periodicals, Inc.

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Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River

Cited by 27 publications

References 61 publications

Evaluation of Genotoxic Pressure along the Sava River

Evaluation of Genotoxic Pressure along the Sava River

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

Contamination and genotoxicity biomarker responses in bivalve mussels from the major Lithuanian rivers

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