Flocculation tests with electrophoretically separated serum proteins

Maclagan, N. F.; Bunn, David N.

doi:10.1042/bj0410580

Cited by 68 publications

(20 citation statements)

References 7 publications

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“…These findings, while of interest, are not unexpected in view of the complexity of these reactions. Recent investigations (3,5,(22)(23)(24)(25)(26) have shed some light on the mechanisms of these tests, such as the evidence for the role of lipo-protein of the beta-globulin fraction in the thymol turbidity test. Nevertheless, many problems of the precise interactions of the proteins remain to be clarified.…”

Section: Discussionmentioning

confidence: 99%

Electrophoretic Studies of the Serum Proteins in Virus Hepatitis

Ricketts¹,

Sterling²

1949

J. Clin. Invest.

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Section: Discussionmentioning

confidence: 99%

Electrophoretic Studies of the Serum Proteins in Virus Hepatitis

Ricketts¹,

Sterling²

1949

J. Clin. Invest.

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“…tests, notwithstanding the various interpretations advanced to explain the mechanisms of these tests (16)(17)(18)(19)(20)(21)(22).…”

mentioning

confidence: 99%

The Serum Proteins in Infectious Mononucleosis. Electrophoretic Studies

Sterling¹

1949

J. Clin. Invest.

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Previous investigations have shown the very frequent occurrence in infectious mononucleosis of abnormal findings on liver function tests (1-7), and of hepatic involvement on histologic examination of both autopsy (8-11) and biopsy material (1, 12-14) in cases with and without jaundice.Alterations observed in the composition of the serum proteins (2, 4, 6, 15, 16a) appear to be compatible with the occurrence of positive cephalin-cholesterol flocculation and thymol turbidity

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“…Many consist of modifications of the classical Tiselius apparatus so that zones containing specific migrating components may be removed without seriously disturbing the equilibrium of the remaining migrating boundaries (Maclagan and Bunn, 1947;Svensson, 1946;Kekwick, Lyttleton, Brewer, andDreblow, 1951 Crook et al, 1952 Svensson's method a stream of buffer electrolyte is fed by gravity across a filter paper and at right angles to an electrical current passed through the filter paper. If a solution of a mixture of proteins is allowed to flow gently from a point on the top edge of the paper it will be washed slowly down with the stream of buffer, moving under the influence of gravity.…”

Section: Filter-paper Electrophoresismentioning

confidence: 99%

The Use and Limitations of Filter-paper Electrophoresis

Martin¹,

Franglen²

1954

Journal of Clinical Pathology

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In 1935 Tiselius, using the apparatus he had devised in 1930, demonstrated that human serum globulin fell into three reasonably defined groups based on their electrophoretic mobilities. For the next 15 years a steady flood of papers reported the fluctuations of albumin and the globulins in young and old, in health and disease, as disclosed by analyses carried out using Tiselius apparatus with and without the numerous minor modifications which were introduced.In spite of brilliant efforts at simplification it was evident that the method, because of its relative complexity, could never have widespread use in routine clinical chemistry. In 1950 three groups of workers, among them Tiselius himself, described methods which indicated that, using filter paper as a matrix, a relatively cheap and rapid method could be evolved of obtaining a rough analysis of the serum proteins akin to the analysis obtained by the classical technique.The method required no more than 0.5 ml. of serum, and though estimates of the total protein concentration would still have to be made by other means, it was conceivable that this simple technique would dispense with the tedious salt fractionation and subsequent analyses required heretofore to obtain the albumin/globulin ratio.The last four years have produced a spate of papers devoted to the description of modifications in methods of separation and analysis with numerous technical and mathematical tricks for improving the correlation of the results with the substantial body of data already obtained with the classical technique. With few notable exceptions scant attention was paid to the fundamental principles underlying the technique or to the points in which their application to it differed from the classical procedure.As paper succeeded paper it became increasingly evident that except within fairly wide limits the two methods were not quantitatively comparable.Even worse, because of lack of time spent on a consideration of the basic principles involved, G it seemed probable that differing techniques for the production of " electrophoretograms " were not comparable among themselves.One was faced with the prospect of scores of laboratories turning out hundreds of results year by year; each group, because of deviations in technique unappreciated and therefore unrecorded, differing to an unknown extent one from the other. The clinical chemical chaos that could arise demanded a reassessment of the situation in the hope that workers in the field would stop, think, and start again.Electrokinetic phenomena, as the derivation of the word implies, are concerned with the

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Flocculation tests with electrophoretically separated serum proteins

Cited by 68 publications

References 7 publications

Electrophoretic Studies of the Serum Proteins in Virus Hepatitis

Electrophoretic Studies of the Serum Proteins in Virus Hepatitis

The Serum Proteins in Infectious Mononucleosis. Electrophoretic Studies

The Use and Limitations of Filter-paper Electrophoresis

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