Flocculant production from kerosene

Abstract

SummaryCultivation of Corynebaeterium hydrocarboclastus, which is capable of synthesizing an extracellular polymer and utilized hydrocarbons, has been reported. Growth studies in shake flasks and fermenters were made to obtain maximum polymer production. Polymer formation was found to be growth associated. The highest level of polymer accumulation was attained after 50-60 hr cultivation in the fermenter and it amounted to approximately 5.5-6 g/liter of fermentation broth. The medium contained initially 2% (v/v) kerosene as a carbon source. The maximum yield obtained corresponds to 37-40% (w/w) of kerosene supplied. At the same time the cell concentration was 10-13 g/liter which represents the yield of 6 7 4 7 % (w/w). .The rate of polymer production in the exponential phase was 0.25 g/liter hr and cell production rate was 0.27 g/liter hr. Sodium nitrate, 0.5%, and yeast extract, 0.3Yc, (w/w) were the best nigrogen sources for polymer formation. The highest level of polymer produced in broth was 6 g/liter.

Technical aspects of the rheological properties of microbial cultures

“…In all these cases, monotonic increase of viscosity with reaction time is reported, but basic rheological behavior is not discussed. Similar reports are available for other microbial polysaccharide-containing culture broths [67,70]. Published rheological properties of culture fluids containing microbial polysaccharides are summarized in Table 2.…”

Selection and Application of Microorganisms to Improve Oil Recovery

“…100 lL of each activated culture was inoculated in 10 mL of mineral media [28] modified by adding yeast extract 0.1 g/L and glucose 0.5 g/L (MMM). Consortium 1 consisted of a mixture of Bacillus polymyxa, Bacillus brevis, Micrococcus varians, Micrococcus sp., and Pseudomonas aeruginosa strains 38H and 44C, Vibrio sp.…”

“…The control did not contain bacteria. 100 lL of each activated culture was inoculated in 10 mL of mineral media [28] modified by adding yeast extract 0.1 g/L and glucose 0.5 g/L (MMM). Each strain grew overnight at 35 C shaking routinely in the same MMM.…”

Rapid method for monitoring maximum biosurfactant production obtained by acetone precipitation

“…The organisms were grown in 50 ml mineral medium containing 1% F dodecane in 500 ml shaken conical flasks at 120 rpm at 30°C. Modified miner-l medium of Knetting and Zajic (Knetting and Zajic, 1972) was used for cultivation of the strains. The medium contained KH2P04, 2g; (NH4)2 SOq, 3g; NaN03, 2g; NaCI, O.lg; MgSy4.…”