Floatation, a Bulk-Selection Method for Breeders which Permits the Selection of Faster Modifying Barley Grains

Briggs, D. E.; Sole, S. M.; Bury, Paul

doi:10.1002/j.2050-0416.2001.tb00086.x

Cited by 4 publications

(2 citation statements)

References 5 publications

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“…With many breeding systems this means that much grain is discarded in each generation after a cross has been made although, statistically, good malting quality strains are likely to be present in the discarded material. We (Dennis Briggs, Stan Sole of the Crisp Malting Group and Paul Bury of New Farm Crops) have some evidence that it is possible 116 . H.T.…”

Section: Sqixlmrk 2i[mentioning

confidence: 99%

Malt Modification - A Century Of Evolving Views

Briggs

2002

Journal of the Institute of Brewing

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This lecture is the 22nd to commemorate Horace Taberrer Brown, a truly remarkable polymath who made important contributions to brewing practice and science and to 'pure' science. The ideas that Brown and his collaborators developed in the 1890s regarding the origins of the enzymes which catalyse the modification of the endosperm of germinating barley and how modification progresses through the grain are summarized, then the stages by which our understanding has been enhanced are noted. The discovery of gibberellins, their use in malting, the role they play in germinating grain, and how this knowledge has suggested ways to make malts with reduced malting losses are outlined. The discrepant reported patterns of modification and true 'average' patterns are described. It is pointed out that abrasion does not work by inducing two-way modification, as originally proposed. This successful process probably depends on improved access of oxygen to the living grain tissues. Microbes on the grain compete with the embryo and aleurone layer for oxygen, to the detriment of germination and modification. Commercially acceptable ways in which the activities of microbes might be reduced (and malting enhanced) are discussed, including the use of plug-rinses at the end of steeps to reduce the microbial population and the quantity of the microbial nutrients which support growth and respiration and are dissolved in the water around the grains. A newly-proposed method for the bulk-selection of malting quality lines from mixed populations of barley is mentioned. Finally, the dangers to the malting and brewing industries in the U.K. of the continuing reduction in scientific research is indicated.

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Section: Sqixlmrk 2i[mentioning

confidence: 99%

Malt Modification - A Century Of Evolving Views

Briggs

2002

Journal of the Institute of Brewing

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“…Grain density is a measure of the compactness of a seed. Grain with a lower density has a mealy endosperm that contains loosely packed starch granules in a discontinuous protein matrix interspersed with vacuous spaces (Nair et al 2010;Psota et al 2007;Nair et al 2011;Briggs et al 2001;Chang 1988;Fang and Campbell 2000). Physical traits, such as grain weight and size, are used routinely to classify the harvest quality of barley.…”

Section: Introductionmentioning

confidence: 99%

The detection of QTLs in barley associated with endosperm hardness, grain density, grain size and malting quality using rapid phenotyping tools

et al. 2013

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Using a barley mapping population, 'Vlamingh' × 'Buloke' (V × B), whole grain analyses were undertaken for physical seed traits and malting quality. Grain density and size were predicted by digital image analysis (DIA), while malt extract and protein content were predicted using near infrared (NIR) analysis. Validation of DIA and NIR algorithms confirmed that data for QTL analysis was highly correlated (R (2) > 0.82), with high RPD values (the ratio of the standard error of prediction to the standard deviation, 2.31-9.06). Endosperm hardness was measured on this mapping population using the single kernel characterisation system. Grain density and endosperm hardness were significantly inter-correlated in all three environments (r > 0.22, P < 0.001); however, other grain components were found to interact with the traits. QTL for these traits were also found on different genomic regions, for example, grain density QTLs were found on chromosomes 2H and 6H, whereas endosperm hardness QTLs were found on 1H, 5H, and 7H. In this study, the majority of the genomic regions associated with grain texture were also coincident with QTLs for grain size, yield, flowering date and/or plant development genes. This study highlights the complexity of genomic regions associated with the variation of endosperm hardness and grain density, and their relationships with grain size traits, agronomic-related traits, and plant development loci.

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Tetrazolium Staining, Mitochondria, and Barley Quality

Briggs

Sole²,

Latham

2009

Journal of the Institute of Brewing

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Triphenyl tetrazolium chloride (TTC) staining of individual isolated barley aleurone layers was carried out under aseptic conditions and the amount of formazan generated under a standardized incubation period was quantified. The nature of the tests minimized or excluded the influences of grain damage, ageing or contaminating microorganisms. Embryos from selected Triumph barley grains, having intensely or poorly staining aleurone layers, were grown on to maturity. Collected grain was analysed and embryos from the highest-staining progeny from the highstaining aleurone line and the lowest-staining progeny of the low-staining aleurone line were grown on to yield grain of the second selected generations. Aleurone layers of the high-staining line continued to stain intensely, respired strongly, and generated large amounts of α-amylase in response to gibberellic acid and embryos from these grains respired and grew vigorously, as did whole grains. In contrast, the aleurone layers of grains from the low-staining line continued to stain poorly and respired weakly, generated little α-amylase in response to gibberellic acid, the embryos respired slowly and lacked vigour and, like the whole grains, grew poorly. It is proposed that these results are the consequence of the selection procedure separating lines with superior and inferior mitochondria. If this apparently novel approach using TTC staining is confirmed as being valid, it opens a new way for plant breeders to select for more vigorous cereal lines.

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Floatation, a Bulk-Selection Method for Breeders which Permits the Selection of Faster Modifying Barley Grains

Cited by 4 publications

References 5 publications

Malt Modification - A Century Of Evolving Views

Malt Modification - A Century Of Evolving Views

The detection of QTLs in barley associated with endosperm hardness, grain density, grain size and malting quality using rapid phenotyping tools

Tetrazolium Staining, Mitochondria, and Barley Quality

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