Flexible Simultaneous Mesoscale Two-Photon Imaging Of Neural Activity at High Speeds

Abstract

Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.

Reliable determination of pulse-shape instability in trains of ultrashort laser pulses using frequency-resolved optical gating

“…Also, pump-laser stability is a critical factor for achieving stable supercontinuum in optical fibers, particularly hollow core fibers 14,15 . Ultrashort laser pulses are now also widely used in medical [16][17][18][19][20] and industrial applications 21 for surgical, imaging, and micro-fabrication purposes. Some examples are two photon scanning microscopy, ophthalmology, ultrafast…”