Flexible biofilm monitoring device

Reiferth, Viktoria Maria; Holtmann, Dirk; Müller, Daniela

doi:10.1002/elsc.202100076

Cited by 2 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the establishment of the Calgary assay modified by Reiferth et al ( 2022 ) for the SRB D. vulgaris , all growth‐critical steps were performed in an anaerobic chamber, and only the crystal violet staining was performed under aerobic conditions. All required fluids were flushed with N 2 .…”

Section: Discussionmentioning

confidence: 99%

“…The optimization of this assay, with the use of microcentrifuge tubes as growth surfaces in the respective wells, ensures flexibility to the respective conditions, as well as high throughput at the same time. Additionally, this method is more economical than the Calgary Biofilm Device and can be implemented with common laboratory equipment (Reiferth et al, 2022 ). It can be used to determine the minimum biofilm inhibitory concentration (MBIC), as well as the minimum biofilm eradication concentration (MBEC).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antibiofilm assay for antimicrobial peptides combating the sulfate‐reducing bacteria Desulfovibrio vulgaris

Stillger,

Viau,

Holtmann

et al. 2023

MicrobiologyOpen

Self Cite

View full text Add to dashboard Cite

In medical, environmental, and industrial processes, the accumulation of bacteria in biofilms can disrupt many processes. Antimicrobial peptides (AMPs) are receiving increasing attention in the development of new substances to avoid or reduce biofilm formation. There is a lack of parallel testing of the effect against biofilms in this area, as well as in the testing of other antibiofilm agents. In this paper, a high‐throughput screening was developed for the analysis of the antibiofilm activity of AMPs, differentiated into inhibition and removal of a biofilm. The sulfate‐reducing bacterium Desulfovibrio vulgaris was used as a model organism. D. vulgaris represents an undesirable bacterium, which is considered one of the major triggers of microbiologically influenced corrosion. The application of a 96‐well plate and steel rivets as a growth surface realizes real‐life conditions and at the same time establishes a flexible, simple, fast, and cost‐effective assay. All peptides tested in this study demonstrated antibiofilm activity, although these peptides should be individually selected depending on the addressed aim. For biofilm inhibition, the peptide DASamP1 is the most suitable, with a sustained effect for up to 21 days. The preferred peptides for biofilm removal are S6L3‐33, in regard to bacteria reduction, and Bactenecin, regarding total biomass reduction.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%